2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycin
1994
Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells
Sumpio B, Widmann M, Ricotta J, Awolesi M, Watase M. Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. Journal Of Cellular Physiology 1994, 158: 133-139. PMID: 8263020, DOI: 10.1002/jcp.1041580117.Peer-Reviewed Original ResearchConceptsSystemic blood pressureCultured endothelial cellsBlood pressureSystemic pressureCell responsesEndothelial cell responsesBovine aortic ECsMorphologic changesEndothelial cellsAortic ECsRat type IEC phenotypeSignificant increaseCell numberEC proliferationVascular systemSignificant regulatory influenceCalf serumEndotheliumType IDigital monitorRegulatory influenceProliferationHg pressureCells
1992
Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro.
Rosales OR, Sumpio BE. Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro. Surgery 1992, 112: 459-66. PMID: 1379381.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCImmunocytochemical stainingPKC activityCyclic stretchEndothelial cell activationVascular endothelial cellsCycles/minSustained elevationCell activationFlexible-bottomed culture platesKinase CBovine aortic ECsECS resultsEndothelial cellsBlood vesselsAortic ECsEvidence of translocationSpecific PKC inhibitorEC proliferationCalphostin CEC growthHemodynamic forces