2004
The identification of novel RNA structural motifs using COMPADRES: an automated approach to structural discovery
Wadley LM, Pyle AM. The identification of novel RNA structural motifs using COMPADRES: an automated approach to structural discovery. Nucleic Acids Research 2004, 32: 6650-6659. PMID: 15608296, PMCID: PMC545444, DOI: 10.1093/nar/gkh1002.Peer-Reviewed Original Research
1994
Building a kinetic framework for group II intron ribozyme activity: quantitation of interdomain binding and reaction rate.
Pyle AM, Green JB. Building a kinetic framework for group II intron ribozyme activity: quantitation of interdomain binding and reaction rate. Biochemistry 1994, 33: 2716-25. PMID: 8117737, DOI: 10.1021/bi00175a047.Peer-Reviewed Original ResearchConceptsGeneral base catalysisChemical stepLinear rangeMichaelis-Menten mechanismSplice-site hydrolysisMultiple-turnover kinetic analysesPH/rate profileEnergetic stabilizationBase catalysisActive siteGel filtration chromatographyRibozyme kineticsReaction rateFiltration chromatographySpecific hydrolysisKinetic frameworkKinetic analysisHydrolysisReactionLower Km valuesBase pairingRibozyme activityKcat