2020
Visualizing group II intron dynamics between the first and second steps of splicing
Manigrasso J, Chillón I, Genna V, Vidossich P, Somarowthu S, Pyle AM, De Vivo M, Marcia M. Visualizing group II intron dynamics between the first and second steps of splicing. Nature Communications 2020, 11: 2837. PMID: 32503992, PMCID: PMC7275048, DOI: 10.1038/s41467-020-16741-4.Peer-Reviewed Original ResearchConceptsGroup II intron splicingGroup II intronsSelf-splicing ribozymesGene-editing toolsIntron dynamicsEukaryotic spliceosomeActive site dynamicsIntron splicingRetrotransposable elementsCatalytic triadSplicingMolecular machinesConformational changesFirst residueMultiple conformationsSite dynamicsSpliceosomeIntronsStructural rearrangementsX-ray crystallographyEnzymatic assayStructural dataEnzymatic strategyFunctional dataActive site
2004
Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif
Sigel RK, Sashital DG, Abramovitz DL, Palmer AG, Butcher SE, Pyle AM. Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif. Nature Structural & Molecular Biology 2004, 11: 187-192. PMID: 14745440, DOI: 10.1038/nsmb717.Peer-Reviewed Original ResearchConceptsGroup II intron ribozymeMetal ion titrationsSolution structureMetal ion bindingDivalent metal ionsMetal ion-binding sitesRibozyme active siteBackbone substituentsMetal ionsMajor groove faceIon-binding sitesIon titrationActive siteIon bindingSyn conformationIntron ribozymesMinor groove sideAGC triadNew RNA motifMajor grooveGroove faceStrong Mg2Catalytic triadGroove sideCentral core