Nikolay G Kolev, PhD
Research Scientist in Epidemiology (Microbial Diseases)DownloadHi-Res Photo
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Epidemiology of Microbial Diseases
BCMM 137, 295 Congress Ave
New Haven, CT 06519
United States
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Research Scientist in Epidemiology (Microbial Diseases)
Biography
Nikolay Kolev earned his PhD in Biochemistry from the University of Notre Dame. He was then a postdoctoral associate in the Department of Molecular Biophysics and Biochemistry at Yale University. Together with Dr. Christian Tschudi, he studies various aspects of RNA metabolism in African trypanosomes. The primary focus of Dr. Kolev’s research is the molecular mechanism governing the development of the protozoan parasite Trypanosoma brucei to its infectious metacyclic form, responsible for the transmission of African sleeping sickness and related diseases from the tsetse fly vector to the mammalian host.
Last Updated on April 07, 2025.
Appointments
Epidemiology of Microbial Diseases
Research ScientistPrimary
Other Departments & Organizations
Education & Training
- Postdoctoral Associate
- HHMI, Yale University (2008)
- PhD
- University of Notre Dame (2003)
- MS
- University of Sofia (1997)
Research
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Overview
Medical Research Interests
Antigenic Variation; Gene Expression Regulation; Parasites; Transcriptional Activation; Trypanosoma; Trypanosomiasis, African
Public Health Interests
Disease Transmission; Infectious Diseases; Parasitology; Neglected Tropical Diseases; Trypanosomiasis; Vector-borne Diseases
Research at a Glance
Yale Co-Authors
Frequent collaborators of Nikolay G Kolev's published research.
Publications Timeline
A big-picture view of Nikolay G Kolev's research output by year.
Research Interests
Research topics Nikolay G Kolev is interested in exploring.
Christian Tschudi, PhD
Joan Steitz, PhD
Kazimierz Tycowski, PhD
Saúl Rojas Sánchez, PhD
25Publications
1,340Citations
Gene Expression Regulation
Trypanosomiasis, African
Trypanosoma
Parasites
Publications
2025
Deep mutational scanning of the Trypanosoma brucei developmental regulator RBP6 reveals an essential disordered region influenced by positive residues
Rojas-Sánchez S, Kolev N, Tschudi C. Deep mutational scanning of the Trypanosoma brucei developmental regulator RBP6 reveals an essential disordered region influenced by positive residues. Nature Communications 2025, 16: 1168. PMID: 39885181, PMCID: PMC11782513, DOI: 10.1038/s41467-025-56553-y.Peer-Reviewed Original ResearchCitationsAltmetric
2019
The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA
Kolev NG, Rajan KS, Tycowski KT, Toh JY, Shi H, Lei Y, Michaeli S, Tschudi C. The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA. Journal Of Biological Chemistry 2019, 294: 15559-15574. PMID: 31439669, PMCID: PMC6816085, DOI: 10.1074/jbc.ra119.008580.Peer-Reviewed Original ResearchCitationsAltmetric
2018
Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei
Kolev NG, Ramsdell TK, Tschudi C. Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei. Molecular And Biochemical Parasitology 2018, 226: 20-23. PMID: 30399391, PMCID: PMC6258174, DOI: 10.1016/j.molbiopara.2018.10.003.Peer-Reviewed Original ResearchCitationsAltmetric
2017
The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion
Christiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Molecular Microbiology 2017, 106: 74-92. PMID: 28742275, PMCID: PMC5607103, DOI: 10.1111/mmi.13754.Peer-Reviewed Original ResearchCitationsAltmetricMetacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites
Kolev NG, Günzl A, Tschudi C. Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites. Molecular And Biochemical Parasitology 2017, 216: 52-55. PMID: 28716719, PMCID: PMC5582990, DOI: 10.1016/j.molbiopara.2017.07.002.Peer-Reviewed Original ResearchCitationsThe Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei
Chikne V, Gupta SK, Doniger T, K. SR, Cohen-Chalamish S, Ben-Asher HW, Kolet L, Yahia NH, Unger R, Ullu E, Kolev NG, Tschudi C, Michaeli S. The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei. Journal Of Molecular Biology 2017, 429: 3301-3318. PMID: 28456523, DOI: 10.1016/j.jmb.2017.04.015.Peer-Reviewed Original ResearchCitationsAltmetric
2016
Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitans
Savage AF, Kolev NG, Franklin JB, Vigneron A, Aksoy S, Tschudi C. Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitans. PLOS ONE 2016, 11: e0168877. PMID: 28002435, PMCID: PMC5176191, DOI: 10.1371/journal.pone.0168877.Peer-Reviewed Original ResearchCitationsAltmetricMeSH KeywordsAnimalsElectron Transport Complex IVGene Expression ProfilingGene LibraryHigh-Throughput Nucleotide SequencingInsect VectorsIntestinal MucosaLife Cycle StagesMembrane GlycoproteinsProtozoan ProteinsProventriculusSalivary GlandsSequence Analysis, RNATranscriptomeTrypanosoma brucei bruceiTsetse FliesUp-Regulation
2015
Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA
Eliaz D, Doniger T, Tkacz ID, Biswas VK, Gupta SK, Kolev NG, Unger R, Ullu E, Tschudi C, Michaeli S. Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA. RNA Biology 2015, 12: 1222-1255. PMID: 25970223, PMCID: PMC4829279, DOI: 10.1080/15476286.2015.1038019.Peer-Reviewed Original ResearchCitationsAltmetricSynchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma brucei
Ramey-Butler K, Ullu E, Kolev NG, Tschudi C. Synchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma brucei. Molecular And Biochemical Parasitology 2015, 200: 1-4. PMID: 25896436, PMCID: PMC4470760, DOI: 10.1016/j.molbiopara.2015.04.001.Peer-Reviewed Original ResearchCitationsAltmetric
2014
Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends
Kolev NG, Ullu E, Tschudi C. Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends. Methods In Molecular Biology 2014, 1201: 165-175. PMID: 25388113, DOI: 10.1007/978-1-4939-1438-8_9.ChaptersCitationsAltmetric
Academic Achievements & Community Involvement
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Honors
honor Molecular, Cellular and Immunoparasitology Scientific Award in recognition of scientific excellence
09/15/2009National AwardThe American Society of Tropical Medicine and Hygiene (ASTMH)DetailsUnited States
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Media
- The vault RNA (vtRNA) of Trypanosoma brucei, the unicellular eukaryote causing African sleeping sickness, is shown set against an image of live procyclic form parasites in this cover photo for the October 25, 2019 issue of the Journal of Biological Chemistry.
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Epidemiology of Microbial Diseases
BCMM 137, 295 Congress Ave
New Haven, CT 06519
United States
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Academic Office
Boyer Center for Molecular Medicine
295 Congress Avenue
New Haven, CT 06510