Clotilde Calderwood, PhD
Research Scientist in PharmacologyCards
About
Research
Publications
2026
Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression
Huet-Calderwood C, Fisher O, Das S, Su V, Boggon T, Calderwood D. Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression. Nature Communications 2026, 17: 2719. PMID: 41688454, PMCID: PMC13013565, DOI: 10.1038/s41467-026-69595-7.Peer-Reviewed Original ResearchThis study investigates how two CCM2 proteins must bind a single KRIT1 protein to regulate KLF4 expression, a process critical for vascular health.
2025
Structural basis for MEKK2 dimerization and substrate recognition
Vish K, Huet-Calderwood C, Ha B, Calderwood D, Boggon T. Structural basis for MEKK2 dimerization and substrate recognition. Nature Communications 2025, 17: 193. PMID: 41318559, PMCID: PMC12780230, DOI: 10.1038/s41467-025-66884-5.Peer-Reviewed Original ResearchMAP3Kmitogen-activated protein kinase kinase kinaseC-terminal regionsubstrate recognitionsubstrate targetingdomain interactionskinase domainMEKK2kinase kinasesmall molecule inhibitorsstructural basisMEK5MKK6diverse substratesmolecule inhibitorsMEKK3MAP3phosphorylationK substrateactivation segmentimmune systemMAP3Ksautophosphorylationsubstratedimer
2024
Setdb1 Loss Induces Type I Interferons and Immune Clearance of Melanoma.
McGeary M, Damsky W, Daniels A, Lang S, Xu Q, Song E, Huet-Calderwood C, Lou H, Paradkar S, Micevic G, Kaech S, Calderwood D, Turk B, Yan Q, Iwasaki A, Bosenberg M. Setdb1 Loss Induces Type I Interferons and Immune Clearance of Melanoma. Cancer Immunology Research 2024, 13: 245-257. PMID: 39589394, DOI: 10.1158/2326-6066.cir-23-0514.Peer-Reviewed Original ResearchT cell infiltrationMHC-I expressiontype I interferonimmune clearanceCD8+ T cell-dependent mannerincreased CD8+ T cell infiltrationCD8+ T cell infiltrationdecreased MHC-I expressionanti-cancer immune responseT cell-dependent mannerCD8+ T cellsdecreased T-cell infiltrationcomplete tumor clearanceimmunity to melanomaincreased melanoma growthinflamed tumor microenvironmentloss of SETDB1type I interferon receptortreatment of melanomaType I Interferon Signalingwhole-genome CRISPR screenendogenous retrovirusestype I interferon expressionmetastatic diseasetumor clearance
2023
Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton
Amiri S, Muresan C, Shang X, Huet-Calderwood C, Schwartz M, Calderwood D, Murrell M. Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton. Nature Communications 2023, 14: 8011. PMID: 38049429, PMCID: PMC10695988, DOI: 10.1038/s41467-023-43612-5.Peer-Reviewed Original ResearchConceptsF-actin architecturestress fiberscortical actinactin cytoskeletonmolecular tension sensorsF-actin stress fibersF-actin cytoskeletonfilamentous actin cytoskeletonmechanical forcestension sensorcell divisioncytoskeletoncell migrationextracellular matrixmyosin inhibitionactindirection of stretchcellscell axisuniaxial stretchstretchFRETUse of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis
Huet-Calderwood C, Rivera-Molina F, Toomre D, Calderwood D. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis. Methods In Molecular Biology 2023, 2608: 17-38. PMID: 36653699, PMCID: PMC9999384, DOI: 10.1007/978-1-0716-2887-4_2.ChaptersConceptsβ1 integrintotal internal reflection fluorescence microscopynormal cell adhesionintegrin adhesion receptorsreflection fluorescence microscopyadhesion receptorscell adhesionendocytosisfluorescence microscopyexocytosisintegrinscellsHaloTagpHluorinintracellular labelingectophotobleachingtagsreceptorschasefluorescentadhesionlabelingmigration
2022
Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II–dependent fashion
Huet-Calderwood C, Rivera-Molina F, Toomre D, Calderwood D. Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II–dependent fashion. Journal Of Cell Biology 2022, 222: e202204100. PMID: 36416725, PMCID: PMC9699186, DOI: 10.1083/jcb.202204100.Peer-Reviewed Original ResearchConceptsFN secretionfocal adhesion dynamicsextracellular matrixfocal adhesion formationsites of exocytosislive-cell microscopyintegrin-independent mannercytoskeletal dynamicsfocal adhesionsadhesion dynamicsregulatory componentsmyosin IIintact microtubulescell polarizationcell adhesionintegrin receptorsFN depositionlamellipodiamicrotubulesfibronectinadhesion formationnew adhesion formationfibroblastsII-dependent fashioncellsTousled-like kinase 2 targets ASF1 histone chaperones through client mimicry
Simon B, Lou HJ, Huet-Calderwood C, Shi G, Boggon TJ, Turk BE, Calderwood DA. Tousled-like kinase 2 targets ASF1 histone chaperones through client mimicry. Nature Communications 2022, 13: 749. PMID: 35136069, PMCID: PMC8826447, DOI: 10.1038/s41467-022-28427-0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceCatalytic DomainCell Cycle ProteinsConserved SequenceCrystallography, X-RayHistonesHumansMolecular ChaperonesMolecular Docking SimulationMolecular MimicryMutagenesisPeptide LibraryPhosphorylationProtein KinasesRecombinant ProteinsSubstrate SpecificityConceptsTousled-like kinaseDNA replication-coupled nucleosome assemblynuclear serine-threonine kinasereplication-coupled nucleosome assemblyhistone chaperone proteinsglobular N-terminal domainproper cell divisionphosphorylation site motifsserine-threonine kinaseshort sequence motifsAsf1 histone chaperonesC-terminal tailN-terminal domainhistone chaperonesgenome maintenancenucleosome assemblysequence motifschaperone proteinsnon-catalytic interactionscatalytic domaincell divisionsite motifN-terminusstringent selectivitycell growth
2020
Differences in self-association between kindlin-2 and kindlin-3 are associated with differential integrin binding
Kadry YA, Maisuria EM, Huet-Calderwood C, Calderwood DA. Differences in self-association between kindlin-2 and kindlin-3 are associated with differential integrin binding. Journal Of Biological Chemistry 2020, 295: 11161-11173. PMID: 32546480, PMCID: PMC7415974, DOI: 10.1074/jbc.ra120.013618.Peer-Reviewed Original ResearchConceptskindlin-3Kindlin-2focal adhesionsintegrin cytoplasmic domaintransmembrane adhesion receptorscomparative sequence analysislive-cell imagingability of cellscytoplasmic domainF3 subdomainsmammalian cellscytoplasmic componentsextracellular environmentadhesion receptorskindlinsequence analysisintegrin familyself-associationintegrin bindingphysiological importancemolecular levelpoint mutationsproteincellsadhesion
2018
Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading
Kadry YA, Huet-Calderwood C, Simon B, Calderwood DA. Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading. Journal Of Cell Science 2018, 131: jcs221184. PMID: 30254023, PMCID: PMC6215391, DOI: 10.1242/jcs.221184.Peer-Reviewed Original ResearchConceptsintegrin-linked kinasefocal adhesion localizationKindlin-2cell spreadingintegrin-mediated signalingILK bindingILK mutantpseudokinase domainintegrin signalingknockdown cellsaxis downstreamC-lobecell morphologymutantssignalingcentral rolePKDcomplete understandinglocalizationfirst personkinaseadaptorsitesspeciesintegrinsStructural basis of the filamin A actin-binding domain interaction with F-actin
Iwamoto DV, Huehn A, Simon B, Huet-Calderwood C, Baldassarre M, Sindelar CV, Calderwood DA. Structural basis of the filamin A actin-binding domain interaction with F-actin. Nature Structural & Molecular Biology 2018, 25: 918-927. PMID: 30224736, PMCID: PMC6173970, DOI: 10.1038/s41594-018-0128-3.Peer-Reviewed Original ResearchConceptsactin-binding domaincalponin homology domainhomology domainF-actinactin cross-linking proteinfunction mutationstandem calponin homology domainsdisease-associated mutantscryo-electron microscopyhigh-resolution structuresnumerous genetic diseasessequence conservationhigher-order structurelinking proteinstructural basisdomain interactionscell shapeactin filamentsmolecular understandingN-terminalfunctional studiesgenetic diseasesmissense mutationsmutationsatomic resolution