Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii
Maher N, Vermund S, Lasbury M, Lee C, Bartlett M, Unnasch T. Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii. Journal Of Clinical Microbiology 2000, 38: 1947-1952. PMID: 10790126, PMCID: PMC86630, DOI: 10.1128/jcm.38.5.1947-1952.2000.Peer-Reviewed Original ResearchConceptsPneumocystis carinii pneumoniaRespiratory specimensP. cariniiViable trophozoitesPatient respiratory specimensP. carinii DNAOpportunistic illnessesViability assaysCarinii pneumoniaPCP patientsShock protein 70 familyReverse transcription-PCRHeat shock protein 70 familyPneumocystis cariniiNoninvasive techniqueCarinii DNACariniiTranscription-PCREthidium bromide stainingOptimal assay conditionsTrophozoitesBromide stainingAssaysMolecular viabilitySpecific primers