Specific Induction of Tumor Neovasculature Death by Modified Murine PPE-1 Promoter Armed with HSV-TK
Varda-Bloom N, Hodish I, Shaish A, Greenberger S, Tal R, Feder B, Roitelman J, Breitbart E, Bangio L, Barshack I, Pfeffer R, Harats D. Specific Induction of Tumor Neovasculature Death by Modified Murine PPE-1 Promoter Armed with HSV-TK. Pathobiology 2008, 75: 346-355. PMID: 19096230, DOI: 10.1159/000164219.Peer-Reviewed Original ResearchConceptsHSV-TKSimplex virus thymidine kinaseHSV-TK geneTherapeutic genesVirus thymidine kinaseGene therapyMurine preproendothelin-1 promoterAdenoviral vectorPreproendothelin-1 promoterReporter geneTissue specificityTumor neovasculaturePromoterSpecific inductionGenesHigh expressionTumor angiogenesisMetastasis developmentExpressionNeovasculatureMouse modelKinaseSelectivityEfficiencySpecificityEndothelial-targeted gene transfer of hypoxia-inducible factor-1alpha augments ischemic neovascularization following systemic administration.
Tal R, Shaish A, Rofe K, Feige E, Varda-Bloom N, Afek A, Barshack I, Bangio L, Hodish I, Greenberger S, Peled M, Breitbart E, Harats D. Endothelial-targeted gene transfer of hypoxia-inducible factor-1alpha augments ischemic neovascularization following systemic administration. Molecular Therapy 2008, 16: 1927-36. PMID: 18797451, DOI: 10.1038/mt.2008.191.Peer-Reviewed Original ResearchConceptsHypoxia-inducible factor-1alphaSystemic administrationTherapeutic efficacyProangiogenic gene therapyFactor-1alphaSystemic side effectsEnhanced blood perfusionRoute of administrationTail vein administrationHindlimb ischemia modelMouse hindlimb ischemia modelMurine preproendothelin-1 promoterClinical outcomesPreproendothelin-1 promoterC57BL/6 miceIschemic neovascularizationIntravenous routeIschemic muscleIntraarterial routeIschemia modelCapillary densityIschemic regionSide effectsSystemic toxicityTherapeutic angiogenesisAntiangiogenic systemic gene therapy combined with doxorubicin administration induced caspase 8 and 9-mediated apoptosis in endothelial cells and an anti-metastasis effect
Peled M, Shaish A, Greenberger S, Katav A, Hodish I, Ben-Shushan D, Barshack I, Mendel I, Frishman L, Tal R, Bangio L, Breitbart E, Harats D. Antiangiogenic systemic gene therapy combined with doxorubicin administration induced caspase 8 and 9-mediated apoptosis in endothelial cells and an anti-metastasis effect. Cancer Gene Therapy 2008, 15: 535-542. PMID: 18421310, DOI: 10.1038/cgt.2008.20.Peer-Reviewed Original Research