Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding
Kayisli O, Kayisli UA, Basar M, Semerci N, Schatz F, Lockwood CJ. Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding. PLOS ONE 2015, 10: e0137855. PMID: 26436918, PMCID: PMC4593551, DOI: 10.1371/journal.pone.0137855.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsMedroxyprogesterone acetateGuinea pigsUterine bleedingCultured human endometrial stromal cellsStromal cellsGlandular cellsProgestin only contraceptivesEndometrial blood flowOvariectomized guinea pigsMajor side effectsEffective family planning methodsGlucocorticoid receptor activationGR transcriptional activityFragile blood vesselsFamily planning methodsIngenuity Pathway AnalysisFKBP51 expressionFunctional withdrawalMicroarray resultsOnly contraceptivesGlucocorticoid withdrawalEndometrial inflammationLong-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival
Kayisli UA, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson HC, Shapiro J, Summerfield T, Huang SJ, Prelle K, Schatz F, Lockwood CJ. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 5153-5158. PMID: 25847994, PMCID: PMC4413279, DOI: 10.1073/pnas.1424814112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CountCell DifferentiationCell MovementCell ProliferationCell SurvivalCells, CulturedChemokine CCL2Contraceptive Agents, FemaleDesogestrelEndometriumFemaleGene Expression ProfilingGene Expression RegulationGuinea PigsHumansMedroxyprogesterone AcetateModels, BiologicalMuscle, Smooth, VascularMyocytes, Smooth MuscleOvariectomyProgestinsProliferating Cell Nuclear AntigenSignal TransductionSTAT1 Transcription FactorConceptsVascular smooth muscle cellsChemokine ligand 2Medroxyprogesterone acetateEndometrial vasculatureVSMC proliferationCultured vascular smooth muscle cellsSmooth muscle cellsSmooth muscle cell survivalVascular smooth muscle cell survivalCell nuclear antigenFragile microvesselsΑSMA immunoreactivityEndometrial vesselsΑSMA stainingPericyte proliferationSTAT1 inhibitorGuinea pigsMuscle cellsMuscle cell survivalCCL2 effectsEtonogestrelMigration assaysNuclear antigenVSMC differentiationWomen