2016
Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility
Samanta L, Swain N, Ayaz A, Venugopal V, Agarwal A. Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility. Biochimica Et Biophysica Acta 2016, 1860: 1450-1465. PMID: 27062907, DOI: 10.1016/j.bbagen.2016.04.001.Peer-Reviewed Original ResearchConceptsPost-translational modificationsSperm proteinsSperm functionOccurrence of post-translational modificationsExpressed protein complementHuman sperm proteinsProtein synthesis machineryAnalysis of post-translational modificationsComplex interaction networksProteome diversificationSperm proteomeFemale reproductive tractHuman spermatozoaProtein complementStructure-function relationshipsSynthesis machineryInteraction networkProtein stabilityMale infertilitySpermMale factor infertilitySpermatozoaProtein profilesDifferential occurrenceFertility potential
2015
Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species
Agarwal A, Ayaz A, Samanta L, Sharma R, Assidi M, Abuzenadah A, Sabanegh E. Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species. Clinical Proteomics 2015, 12: 23. PMID: 26321892, PMCID: PMC4552280, DOI: 10.1186/s12014-015-9094-5.Peer-Reviewed Original ResearchMembrane metallo-endopeptidaseInfertile menSeminal plasmaSperm functionFertile menLTQ-Orbitrap Elite hybrid mass spectrometer systemProtein foldingNatural reservoir of antioxidantsMale infertilityROS levelsComparison to fertile controlsSeminal plasma of infertile menPost-translational modification of proteinsHigh ROS groupImpairment of sperm functionPathology of male infertilitySeminal plasma proteinsDiagnosis of male infertilityFunction of reactive oxygen speciesMotility of spermPost-translational modificationsFertile groupModification of proteinsInfertile patient groupsOxygen speciesSpermatozoa protein alterations in infertile men with bilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Spermatozoa protein alterations in infertile men with bilateral varicocele. Asian Journal Of Andrology 2015, 18: 43-53. PMID: 25999357, PMCID: PMC4736356, DOI: 10.4103/1008-682x.153848.Peer-Reviewed Original ResearchConceptsInfertile menBilateral varicoceleFertile menAssociated with metabolic processesSperm-zona bindingVaricocele casesFunctional proteomic profilingVaricocele-associated male infertilityImmune system processPresence of DEPSperm-zonaSperm functionFertile control groupCells of patientsFertile donorsEnzyme regulationIdentified proteinsOxidoreductase activityMale infertilitySpermatozoa cellsProteomic analysisWestern blot analysisStress responseMetabolic processesProteomic profiling