2013
Neuroprotective Role of a Brain-Enriched Tyrosine Phosphatase, STEP, in Focal Cerebral Ischemia
Deb I, Manhas N, Poddar R, Rajagopal S, Allan AM, Lombroso PJ, Rosenberg GA, Candelario-Jalil E, Paul S. Neuroprotective Role of a Brain-Enriched Tyrosine Phosphatase, STEP, in Focal Cerebral Ischemia. Journal Of Neuroscience 2013, 33: 17814-17826. PMID: 24198371, PMCID: PMC3818554, DOI: 10.1523/jneurosci.2346-12.2013.Peer-Reviewed Original ResearchConceptsIschemic brain damageStriatal-enriched phosphataseBrain damageNeuroprotective roleBrain injuryP38 MAPK activationSustained p38 MAPK activationIschemic brain injuryFocal cerebral ischemiaOnset of reperfusionHypoxia-reoxygenation injuryP38 MAPKMAPK activationIschemic strokeNeurological deficitsCerebral ischemiaStroke therapyKO miceRat modelP38 MAPK pathwayCultured neuronsNeuronal culturesGenetic deletionSecondary activationInjuryCocaine-Induced Changes of Synaptic Transmission in the Striatum are Modulated by Adenosine A2A Receptors and Involve the Tyrosine Phosphatase STEP
Chiodi V, Mallozzi C, Ferrante A, Chen JF, Lombroso PJ, Di Stasi AM, Popoli P, Domenici MR. Cocaine-Induced Changes of Synaptic Transmission in the Striatum are Modulated by Adenosine A2A Receptors and Involve the Tyrosine Phosphatase STEP. Neuropsychopharmacology 2013, 39: 569-578. PMID: 23989619, PMCID: PMC3895235, DOI: 10.1038/npp.2013.229.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCerebral CortexCocaineCorpus StriatumDopamine Uptake InhibitorsEnzyme InhibitorsGene Expression RegulationHumansIn Vitro TechniquesInhibitory Postsynaptic PotentialsMaleMiceMice, Inbred C57BLMice, KnockoutNeural PathwaysNeuronsProtein Tyrosine Phosphatases, Non-ReceptorReceptor, Adenosine A2ASynaptic TransmissionSynaptosomesVanadatesConceptsEffects of cocaineSynaptic transmissionAdenosine A2A receptorsStriatal-enriched protein tyrosine phosphatasePharmacological actionsA2A receptorsWhole-cell voltage-clamp recordingsA2AR antagonist ZM241385Excitatory post-synaptic currentsCocaine-induced reductionMedium spiny neuronsCocaine-induced changesVoltage-clamp recordingsPost-synaptic currentsA2AR knockout miceCorticostriatal slicesStriatal slicesPsychomotor effectsSpiny neuronsSynaptic mechanismsAntagonist ZM241385Synaptic depressionClamp recordingsBrain areasStriatum
2001
Antibodies against neural, nuclear, cytoskeletal, and streptococcal epitopes in children and adults with Tourette’s syndrome, Sydenham’s chorea, and autoimmune disorders
Morshed S, Parveen S, Leckman J, Mercadante M, Kiss M, Miguel E, Arman A, Yazgan Y, Fujii T, Paul S, Peterson B, Zhang H, King R, Scahill L, Lombroso P. Antibodies against neural, nuclear, cytoskeletal, and streptococcal epitopes in children and adults with Tourette’s syndrome, Sydenham’s chorea, and autoimmune disorders. Biological Psychiatry 2001, 50: 566-577. PMID: 11690591, DOI: 10.1016/s0006-3223(01)01096-4.Peer-Reviewed Original ResearchConceptsTotal antinuclear antibodiesAntinuclear antibodiesAutoimmune disordersTourette syndromeAnticytoskeletal antibodiesAntineural antibodiesStreptococcal infectionT patientsTS patientsBeta-hemolytic streptococcal infectionPrior streptococcal infectionHemolytic streptococcal infectionAntistreptolysin O titerIndirect immunofluorescent assayLevels of immunoreactivityWestern blot techniqueMean rankClinical characteristicsSydenham's choreaO titerPatient groupSC patientsIgG antibodiesIgG classNormal controls
2000
The Dopamine/D1 Receptor Mediates the Phosphorylation and Inactivation of the Protein Tyrosine Phosphatase STEP via a PKA-Dependent Pathway
Paul S, Snyder G, Yokakura H, Picciotto M, Nairn A, Lombroso P. The Dopamine/D1 Receptor Mediates the Phosphorylation and Inactivation of the Protein Tyrosine Phosphatase STEP via a PKA-Dependent Pathway. Journal Of Neuroscience 2000, 20: 5630-5638. PMID: 10908600, PMCID: PMC6772528, DOI: 10.1523/jneurosci.20-15-05630.2000.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCatalytic DomainCorpus StriatumCyclic AMP-Dependent Protein KinasesEnzyme ActivationIn Vitro TechniquesMaleMolecular Sequence DataNeuronsPhosphoproteinsPhosphorus RadioisotopesPhosphorylationProtein Tyrosine PhosphatasesProtein Tyrosine Phosphatases, Non-ReceptorRatsRats, Sprague-DawleyReceptors, Dopamine D1Signal TransductionConceptsProtein tyrosine phosphatase familyCAMP-dependent protein kinaseTryptic phosphopeptide mappingPotential phosphorylation sitesUnique N-terminalProtein-protein interactionsMembrane-associated proteinsRole of phosphorylationTyrosine phosphatase familyAmino acid sequenceSite-directed mutagenesisAmino acid sequencingPKA-dependent pathwayTyrosine phosphatase STEPPhosphatase familyPhosphopeptide mappingPhosphorylation sitesAlternative splicingSubcellular compartmentsProtein kinaseTerminal domainEquivalent residuesCytosolic proteinsSpecific residuesAcid sequence
1995
Molecular Cloning of the Human Homolog of a Striatum-Enriched Phosphatase (STEP) Gene and Chromosomal Mapping of the Human and Murine Loci
Li X, Luna J, Lombroso P, Francke U. Molecular Cloning of the Human Homolog of a Striatum-Enriched Phosphatase (STEP) Gene and Chromosomal Mapping of the Human and Murine Loci. Genomics 1995, 28: 442-449. PMID: 7490079, DOI: 10.1006/geno.1995.1173.Peer-Reviewed Original ResearchConceptsSrc homology domain 3STEP geneTyrosine phosphataseSomatic cell hybrid analysisHuman fetal brain cDNA libraryFetal brain cDNA libraryPhosphatase catalytic domainCell hybrid analysisProtein tyrosine phosphataseAmino acid domainProline-rich regionCandidate disease genesBrain cDNA libraryUsher syndrome type 1CAmino acid levelsChromosomal mappingPhosphatase geneMutant lociHuman homologTrue homologsMolecular cloningCatalytic domainAcid domainMurine locusCDNA libraryCellular and molecular characterization of a brain-enriched protein tyrosine phosphatase
Boulanger L, Lombroso P, Raghunathan A, During M, Wahle P, Naegele. Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase. Journal Of Neuroscience 1995, 15: 1532-1544. PMID: 7869116, PMCID: PMC6577844, DOI: 10.1523/jneurosci.15-02-01532.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAxonsBlotting, WesternBrainCerebral CortexCorpus StriatumImmunohistochemistryIn Situ HybridizationMiceMice, Inbred BALB CNeuronsPeptide FragmentsProtein Tyrosine PhosphatasesProtein Tyrosine Phosphatases, Non-ReceptorRatsRNA, MessengerSynaptic TransmissionTissue DistributionConceptsSubstantia nigraAdult rat brainCombination of immunocytochemistrySitu hybridization studiesProjection neuronsBasal gangliaCaudate putamenPresynaptic axonsStriatal enriched protein tyrosine phosphataseRat brainBrain regionsImmunocytochemical stainingLesion experimentsWestern blotLesion studiesWestern blottingMonoclonal antibodiesMRNA expression patternsImmunoreactive formsImmunoreactive bandsProtein tyrosine phosphataseNigraSitu hybridizationHybridization studiesSTEP isoforms
1993
A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures
Lombroso P, Naegele, Sharma E, Lerner M. A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures. Journal Of Neuroscience 1993, 13: 3064-3074. PMID: 8331384, PMCID: PMC6576687, DOI: 10.1523/jneurosci.13-07-03064.1993.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasal GangliaBase SequenceBlotting, WesternBrainChromatography, AffinityCorpus StriatumDopamine and cAMP-Regulated Phosphoprotein 32FemaleGene ExpressionImmune SeraImmunohistochemistryKineticsMaleMolecular Sequence DataMolecular WeightNerve Tissue ProteinsNeuronsOligodeoxyribonucleotidesOligonucleotides, AntisenseOrgan SpecificityPhosphoproteinsPolymerase Chain ReactionProtein Tyrosine PhosphatasesProtein Tyrosine Phosphatases, Non-ReceptorRatsReceptors, DopamineRecombinant Fusion Proteins
1991
Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum.
Lombroso P, Murdoch G, Lerner M. Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 7242-7246. PMID: 1714595, PMCID: PMC52270, DOI: 10.1073/pnas.88.16.7242.Peer-Reviewed Original ResearchConceptsDeduced amino acid sequenceAmino acid consensus sequenceAmino acid sequenceApparent molecular massRat striatal cDNA librarySingle geneVitro translationCDNA clonesStrong homologyCDNA libraryCytoplasmic membraneAcid sequenceRNA transcriptsN-terminusMolecular characterizationRNA analysisMolecular massAmino acidsProteinPhosphataseMRNASequenceHomologyGenesTerminus