1990
Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function
Eva C, Bovolin P, Balzac F, Botta C, Ricci Gamalero S, Vaccarino F. Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function. Journal Of Molecular Neuroscience 1990, 2: 143. PMID: 2177349, DOI: 10.1007/bf02896839.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylyl CyclasesAnimalsAnimals, NewbornCarbacholCells, CulturedCerebral CortexCholine O-AcetyltransferaseCorpus StriatumKineticsNeuronsPhosphatidylinositolsPolymerase Chain ReactionQuinuclidinyl BenzilateRadioligand AssayRatsReceptors, MuscarinicRNA, MessengerSecond Messenger SystemsConceptsAmount of acetylcholineDensity of mAChRsAdult rat cortexCholinergic receptorsRat cortexNeuronal culturesCholine acetyltransferase-immunoreactive neuronsPrimary culturesMuscarinic cholinergic receptorsTranscriptase-polymerase chain reaction techniqueReverse transcriptase-polymerase chain reaction techniqueNewborn rat brainAdenylate cyclase activityCorticostriatal cellsCorticostriatal neuronsImmunoreactive neuronsSubtype mRNAsChain reaction techniqueMuscarinic antagonistCholinergic transmissionMuscarinic agonistsSecond messenger systemsPostsynaptic componentsNewborn ratsRat brain
1987
Subcellular Location and Neuronal Release of Diazepam Binding Inhibitor
Ferrarese C, Vaccarino F, Alho H, Mellstrom B, Costa E, Guidotti A. Subcellular Location and Neuronal Release of Diazepam Binding Inhibitor. Journal Of Neurochemistry 1987, 48: 1093-1102. PMID: 3819722, DOI: 10.1111/j.1471-4159.1987.tb05632.x.Peer-Reviewed Original ResearchConceptsDBI-like immunoreactivitySlices of hypothalamusCerebral cortical neuronsGamma-aminobutyric acid receptorsRat brain neuronsDiazepam binding inhibitorAllosteric modulatory sitesVeratridine depolarizationNeuronal releaseCerebral cortexMicroM tetrodotoxinSlices of liverPeripheral organsCNS neuronsCortical neuronsBinding of benzodiazepinesCortical astrocytesBrain neuronsNeuromodulatory substancesRat brainSynaptosomal lysatesModulatory siteMet5-enkephalinAcid receptorsNeurons
1985
GABA‐Modulin: A Synaptosomal Basic Protein that Differs from Small Myelin Basic Protein of Rat Brain
Vaccarino F, Tronconi B, Panula P, Guidotti A, Costa E. GABA‐Modulin: A Synaptosomal Basic Protein that Differs from Small Myelin Basic Protein of Rat Brain. Journal Of Neurochemistry 1985, 44: 278-290. PMID: 3964832, DOI: 10.1111/j.1471-4159.1985.tb07142.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsBrain ChemistryCarrier ProteinsChromatography, High Pressure LiquidCyanogen BromideGABA Plasma Membrane Transport ProteinsMembrane ProteinsMembrane Transport ProteinsMuscimolMyelin ProteinsMyelin SheathNerve Tissue ProteinsOrganic Anion TransportersPeptide FragmentsRatsSubcellular FractionsSynaptosomesTissue DistributionConceptsGABA-modulinSmall myelin basic proteinMyelin basic proteinRat brainBasic proteinModulation of GABANeuronal membrane functionNeurotransmitter receptorsSynaptosomal fractionGABA bindingSynaptic membranesSynaptosomal membranesStaphylococcal V8 protease digestionSynaptosomal proteinsNeuronal specificityAcid compositionHigh potencyGABAHigh-affinity bindingBrainMyelinAmino acid compositionTotal synaptosomal proteinApparent molecular weightMembrane function