2013
Functional genomic screen of human stem cell differentiation reveals pathways involved in neurodevelopment and neurodegeneration
Zhang Y, Schulz VP, Reed BD, Wang Z, Pan X, Mariani J, Euskirchen G, Snyder MP, Vaccarino FM, Ivanova N, Weissman SM, Szekely AM. Functional genomic screen of human stem cell differentiation reveals pathways involved in neurodevelopment and neurodegeneration. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 12361-12366. PMID: 23836664, PMCID: PMC3725080, DOI: 10.1073/pnas.1309725110.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsFunctional genomic screensGenomic screenUndifferentiated human embryonic stem cellsNeural lineage developmentSet of genesHuman stem cell differentiationProgenitor cell formationEmbryonic stem cellsStem cell differentiationRNA library screenNeuronal progenitor cellsLate-onset neurodegenerative disorderUnpredicted genesRNA granulesNext-generation sequencingSelf-renewal capacityLineage developmentComplex inheritanceShRNA libraryEarly neurogenesisLibrary screenParallel sequencingCell differentiationGenes
2010
Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates
Silbereis J, Heintz T, Taylor MM, Ganat Y, Ment LR, Bordey A, Vaccarino F. Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates. Molecular And Cellular Neuroscience 2010, 44: 362-373. PMID: 20470892, PMCID: PMC2900521, DOI: 10.1016/j.mcn.2010.05.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAstrocytesBasic Helix-Loop-Helix Transcription FactorsBeta-GalactosidaseCell LineageCerebellumGenes, ReporterGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsIntegrasesMiceMice, Inbred C57BLMice, TransgenicNeurogenesisNeuronsPromoter Regions, GeneticStem CellsTime FactorsConceptsExternal granule cell layerGranule cell precursorsInternal granule cell layerGranule cell layerGranule cellsRhombic lipAstroglial cellsProtein expression profilesGlial fibrillary acidic protein promoterCerebellar granule cell precursorsHuman glial fibrillary acidic protein promoterEmbryonic rhombic lipInducible Cre recombinaseNeuronal progenitor cellsReporter proteinFirst postnatal weekNeural stem cell markersLate embryogenesisCellular plasticityImmature granule cellsEarly postnatal developmentCell layerReporter geneCerebellar granule neuronsStem cell markers
2005
Otx2 Regulates Subtype Specification and Neurogenesis in the Midbrain
Vernay B, Koch M, Vaccarino F, Briscoe J, Simeone A, Kageyama R, Ang SL. Otx2 Regulates Subtype Specification and Neurogenesis in the Midbrain. Journal Of Neuroscience 2005, 25: 4856-4867. PMID: 15888661, PMCID: PMC6724764, DOI: 10.1523/jneurosci.5158-04.2005.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornBasic Helix-Loop-Helix Transcription FactorsBody PatterningBromodeoxyuridineCell CountCell DifferentiationDopamineEmbryo, MammalianEmbryonic InductionFibroblast Growth Factor 8Gene Expression Regulation, DevelopmentalHomeobox Protein Nkx-2.2Homeodomain ProteinsImmunohistochemistryIn Situ HybridizationIn Situ Nick-End LabelingIntermediate Filament ProteinsIntracellular Signaling Peptides and ProteinsKruppel-Like Transcription FactorsMembrane ProteinsMesencephalonMiceMice, TransgenicNerve Tissue ProteinsNestinNeuronsOrganizers, EmbryonicOtx Transcription FactorsPatched ReceptorsReceptors, Cell SurfaceSerotoninTranscription FactorsWnt1 ProteinZebrafish ProteinsZinc Finger Protein GLI1ConceptsMid-hindbrain organizerFunction of Otx2Neuronal subtype identityTranscription factor Otx2Rostral brain developmentEmbryonic day 10.5Cre/loxP systemRole of Otx2Neuronal progenitor cellsMutant embryosProgenitor identityConditional mutantsE10.5 onwardOtx2 activityAnterior hindbrainEctopic expressionCerebellar-like structuresSubtype specificationSubtype identityTransgenic animalsNovel roleLater roleNovel mouse modelOtx2Otx2 expression
2000
Basic Fibroblast Growth Factor (Fgf2) Is Necessary for Cell Proliferation and Neurogenesis in the Developing Cerebral Cortex
Raballo R, Rhee J, Lyn-Cook R, Leckman J, Schwartz M, Vaccarino F. Basic Fibroblast Growth Factor (Fgf2) Is Necessary for Cell Proliferation and Neurogenesis in the Developing Cerebral Cortex. Journal Of Neuroscience 2000, 20: 5012-5023. PMID: 10864959, PMCID: PMC6772267, DOI: 10.1523/jneurosci.20-13-05012.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell DivisionCerebral CortexChoroid PlexusEmbryonic and Fetal DevelopmentFibroblast Growth Factor 2Gene Expression Regulation, DevelopmentalGerm-Line MutationGestational AgeMiceMice, KnockoutProsencephalonReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Fibroblast Growth FactorTelencephalonConceptsFgf2 knockout micePseudostratified ventricular epitheliumKnockout miceCerebral cortexCortical neuronsFrontal cerebral cortexDeep cortical layersBasic fibroblast growth factorEnd of neurogenesisCortical neuron numberNeuronal progenitor cellsNull mutant miceBasic fibroblast growth factor (bFGF) geneFibroblast growth factorDegree of apoptosisLarge neuronsBasal gangliaCortical layersFgf2 knockoutGrowth factor geneMutant miceVentricular epitheliumGermline mutationsNeuron numberNeurogenesis