Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding
Hall S, Clifton L, Sridhar P, Hardy D, Wotherspoon P, Wright J, Whitehouse J, Gamage N, Laxton C, Hatton C, Hughes G, Jeeves M, Knowles T. Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding. Biophysical Journal 2021, 120: 5295-5308. PMID: 34757080, PMCID: PMC8715194, DOI: 10.1016/j.bpj.2021.10.033.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsEscherichia coliEscherichia coli ProteinsProtein FoldingConceptsOuter membrane proteinsBAM complexOuter membraneInserts outer membrane proteinsOuter membrane protein functionsOuter membrane protein foldingOuter membrane of Gram-negative bacteriaMembrane of Gram-negative bacteriaMembrane protein foldingQuartz crystal microbalanceSelf-assembled monolayersGram-negative bacteriaPhospholipid bilayer environmentAssembly machineryProtein foldingCrystal microbalanceMembrane proteinsGold surfaceStructural characterizationBiological functionsPolarized neutron reflectometryDissipation monitoringBilayer environmentComplex foldingProtein