NIAAA R24 Alcohol Research Resources
Mouse models for alcohol metabolism and tissue injury
Major enzymatic pathways involved in ethanol (EtOH), glutathione (GSH) and homocysteine (Hcys) metabolism. CAT, catalase; ADH, alcohol dehydrogenase; CYP2E1, cytochrome P450 2E1; ALDH, aldehyde dehydrogenase; GCL, glutamate-cysteine ligase; GCLC, catalytic subunit of GCL; GCLM, modifier subunit of GCL; ?-GC, ?-glutamylcysteine; Glu, L-glutamate; Gly, glycine; GSSG, glutathione disulfide; NRF2, Nuclear factor erythroid 2-related factor 2; SAM, S-adenosyl-methionine.
Dr. Vasiliou’s laboratory maintains transgenic animal models that are unique and have high relevance to alcohol research. These mice are readily available to researchers by request (see left column). These include:
a) Aldh1a1, Aldh2, Aldh1b1, Cat, Cyp2e1, Adh3a1 and Gclm single knockouts (KO)
b) Aldh1a1 and Aldh3a1 knock-in lines (both have mutated the Cys-302 of the active site)
c) Aldh1a1/Aldh1b1, Aldh1b1/Aldh2, Cat/Cyp2e1 and Gclm/Nrf2 double knockouts (DKO)
d) Conditional Gclc floxed strain
e) Hepatocyte-specific Gclc knockout
Funded by NIAAA (R24AA022057)
Contact
Please send inquiries and requests to:
Dr. Vasilis Vasiliou.
Dr. Vasilis Vasiliou.