The YCGA is a state-of-the-art DNA sequencing facility located on Yale’s West Campus that provides the services, equipment, and expertise required for carrying out large-scale genomics studies and next-generation sequence analysis studies. The YCGA contains over 7,000 sq. feet of custom-designed laboratory and office space equipped with all modern amenities. Yale's West Campus also houses the essential infrastructure - such as uninterrupted and emergency power supplies, high-performance computing clusters (HPC), data storage systems, and a high-speed Ethernet connection for efficient transfer of data - required for DNA sequencing research. The YCGA is well supported by an in-house IT support team consisting of a Systems Administrator and a Systems Technician. Approximately 4,000 sq. feet of the facility is occupied by the laboratory. This space houses both the sequencing equipment and other associated instrumentation and has a dedicated area for library preparation with additional unoccupied space that can be used for future expansion. The office and administrative areas occupy approximately 2,500 sq. feet of space. For safety and experimental reasons, the laboratory area is completely separated from the office space by a glass wall. The laboratory can be accessed from the office area through two glass doors situated at each end of the laboratory. YCGA has 12 offices that are mostly used by PhD level and senior staff. The administrative area is used by the laboratory technicians to carry out computer work and to hold LIMS and other high-end computers used for high-throughput analysis. One conference room with a projector and telecommunication technology provides a venue for interaction with users.
The overall mission of the VBT Program is to foster new research in vascular biology with special emphasis on translational opportunities. We anticipate that discoveries coming from this program will be directed at and applied to clinical problems to improve tissue perfusion. The Program will also train basic and clinician-scientists in research in these disciplines, organize programmatic grants and seminar series and provide an overall intellectual framework for Cardiovascular Research at Yale.
The O’Brien Kidney Center at Yale which includes the Renal Physiology Core providing blood pressure measurements and urine and serum assays, the Mouse Genetics and Cell Line Core providing the generation of kidney-specific cell lines and the generation of in vivo mouse models, and the Human Genetics and Clinical Research Core which focuses on translational studies. In addition, the Yale Translational Research Imaging Center provides a wide range of imaging services including SPECT, microCT, and ultrasound. Transmission electron microscopy (TEM) is available through the Center for Cellular and Molecular Imaging. Metabolic studies in mice are available through the Diabetes Research Center at Yale which can assist with glucose tolerance testing. These facilities are invaluable for the proposed studies.
YARC provides a centralized animal care program with disease surveillance and vendor monitoring. It is fully accredited by the American Association of Accreditation of Laboratory Animal Care (AAALAC) as well as federal and state agencies. Animal facilities are strategically located throughout Yale’s campus including one located in the same building as the PI. YARC provides centralized animal receiving, quarantine, isolation, maintenance, breeding and surgical and necropsy facilities.
The Keck Facility was founded in 1980 as the Yale University School of Medicine Protein and Nucleic Acid Chemistry Facility. In 1987, this unit became associated with HHMI; in 1991, it was moved into 3,200 square feet of space on the third and fourth floors of the newly constructed Boyer Center for Molecular Medicine, whereupon it became the W.M. Keck Foundation Biotechnology Resource Laboratory; and in 1997, it joined with the Mass Spectrometry Resource in the Comprehensive Cancer Center. The PI also has access to the DNA Synthesis Lab, the Flow Cytometry/Cell Sorting Core Facility, the Mass Spectrometry Facility and the Molecular Diagnostics Facility and Yale Center for Genomic Analysis and the Yale Animal Genomics Service Core.