Improving a tool that controls interactions between proteins and organelles expands its use in manipulating signaling in the cell.
Light-inducible dimerization protein modules enable precise temporal and spatial control of biological processes in non-invasive fashion. Among them, Magnets are photoreceptors requiring simultaneous photoactivation to interact, enabling high spatiotemporal confinement of dimerization with a single-excitation wavelength. However, Magnets require concatemerization for efficient responses and cell preincubation at 28oC to be functional. We overcame the limitations by structure-guided protein engineering and validation by cellular assays. The resulting reagents, “enhanced Magnets” (eMags), have greater thermal stability and dimerization efficiency, as well as faster association and dissociation kinetics. We confirmed their effectiveness in several applications including protein recruitment to different organelles, the generation/expansion of organelle contact sites, and the rapid and reversible reconstitution of inter-organelle tethers that have key regulatory function in lipid transport.
- September 16, 2020Source: YaleNews
Two Yale research teams will each receive approximately $9 million in grants from the Aligning Sciences Across Parkinson’s (ASAP) initiative to study the underlying biology of Parkinson’s disease.
- August 20, 2020Source: YaleNews
The two Yale research projects involve the role of mitochondria, the cell’s energy producing factories, in the onset of disease.
- December 16, 2019
Save the Dates: Kavli Institute for Neuroscience Two-day Symposium: "Cell Biology of Parkinson's Disease Genes" on April 28-29
The Kavli Institute for Neuroscience is organizing a two-day symposium, "Cell Biology of Parkinson’s Disease Genes," April 28-29, 2020. Registration required by the February 25, 2020.
- May 23, 2019
Yale’s Pietro De Camilli will be awarded the 2019 Ernst Jung Gold Medal for Medicine by the Jung Foundation for Science and Research on May 23 in Hamburg, Germany.
- August 14, 2018Source: Howard Hughes Medical Institute
Discoveries by two HHMI investigators show how proteins that organize into liquid droplets inside cells make certain biological functions possible.
- August 09, 2018
Several gene mutations have been linked to Parkinson’s disease, but exactly how and where some of them cause their damage has been unclear. A new Yale study, published in The Journal of Cell Biology, shows that one of the genes whose mutations are responsible for a familial form of Parkinson’s encodes a protein that controls the transfer of lipids between membranes of cell organelles.
- July 11, 2018Source: Medicine@Yale
Yale has acquired a major advance in microscopy: the focused ion beam-scanning electron microscope.
- July 05, 2018
Yale researchers found that synaptic vesicles organize themselves into a liquid compartment within the interior of the nerve terminal, much as oil does when mixed with water.
- February 23, 2017Source: Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons
New research adds to growing evidence that Parkinson's disease may arise in part from neurons’ failure to recycle the materials used to package and transport neurotransmitters.