Multimedia

Tomographic EM reconstruction of plasma membrane invaginations enriched in clathrin coated pits at a nerve terminal lacking dynamin 1 and 3. (from: Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takashi J, Cappello V, O’Toole E, Ryan TA, and De Camilli P. 2010. Neuron. 70(6):1100-14.)

Endocytic intermediates generated by the incubation of isolated plasma membrane sheets with brain cytosol. (see: Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, and De Camilli P. 2010. Nat. Cell Biol. 9: 902-908.)

Electron micrograph of a synapse lacking dynamin 1. Note abundance of clathrin coated profiles. (see: Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Paradise S, O’Toole E, Flavell R, Cremona O, Miesenböck, Ryan TA, and De Camilli P. 2007. Science. 316: 570-574.)

Tomographic EM reconstruction of thick sections of synapses from wild type mouse neurons (left), dynamin 1 KO neurons (center) and synaptojanin 1 KO neurons (right). Blue = synaptic vesicles; green = clathrin coated profiles. (from: Hayashi M, Raimondi A, O’Toole E, Paradise S, Collesi C, Cremona O, Ferguson SM, and De Camilli P. 2008. Proc. Nat. Acad. Sci. 105: 2175-2180.)

Electron microscopic image of a synapse in the mouse cerebellum

Electron tomogram (left) and corresponding reconstruction of tubular plasma membrane invaginations capped by clathrin coated pits (red) in a fibroblast the lack dynamin. (from: Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Destaing O, Ko G, Cremona O, O'Toole E, and De Camilli P. 2009. Dev. Cell. 17: 811-822.)

Immunofluorescence of neurons in culture: presynaptic nerve terminals are visualized by anti-synapsin antibodies (green) (from the work of Shawn Ferguson)

Electron micrograph of a synapse lacking both dynamin 1 and 3. Note the abundance of clathrin coated profiles. (from: Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takashi J, Cappello V, O’Toole E, Ryan TA, and De Camilli P. 2010. Neuron. 70(6):1100-14.)

VPS13A and VPS13C are localized at contacts between the ER and either mitochondria or endo/lysosomes respectively. Top: schematic representation showing that both proteins can also localize at contacts between the ER and lipid droplets. Bottom fluorescence image (green: ER; blue: mitochondria or endo/lysosomes respectively; magenta: VPS13)(Kumar, Leonzino et al. 2018, J. Cell Biol 2018, PMID:30093493)2025 Yale School of Medicine. Updated 09/17/2024