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TuKiet Lam, PhD, BS

Research Scientist
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Additional Titles

Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry

Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and Biochemistry

Contact Info

Yale School of Medicine

Keck MS & Proteomics Resource, 300 George Street

New Haven, CT 06510

United States

About

Titles

Research Scientist

Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry; Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and Biochemistry

Biography

TuKiet T. Lam (Tu) earned his undergraduate degree from Rollins College (Winter Park, FL) in 1998. He completed his Ph.D. training at Florida State University in 2002 with mentorship from Alan G. Marshall, Ph.D. After a brief postdoctoral training at the National High Magnetic Field Laboratory (Tallahassee, FL), he joined and headed the FT-ICR Users Facility and worked with Mark R. Emmett Ph.D. there until summer of 2004. He then joined Yale in fall of 2004 as Director to the FT-ICR MS Resource where he utilized his training and expertise in high resolution mass spectrometry to establish his collaborative career in providing state-of-the-art mass spectrometry instrumentation and analyses to investigators from a multitude of disciplines (e.g., Biological, chemical, clinical, pharmacological, environmental, etc.). He currently serves as the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine.

Appointments

Other Departments & Organizations

Education & Training

Postdoctoral Fellow
National High Magnetic Field Laboratory (2003)
PhD
Florida State University, Chemistry/Analytical (2002)
BS
Rollins College, Chemistry (1998)

Research

Overview

I am the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine and have been with Yale since 2004. My specific aims are to train, assist, collaborate, and make available my expertise in mass spectrometry to as many investigators of other scientific and biomedical discipline as possible so that they can advance their research. By establishing a well-organized and well-trained core group of staffs who are dedicated to the same philosophy and mission, my team provides the high-quality mass spectrometry data and analyses which have resulted in co-authorships and acknowledgements in many high impact scientific publications. My mass spectrometry (MS) footing grew from my graduate career at Florida State University, where I developed a hydrogen/deuterium exchange workflow to robustly observe protein-protein interaction(s) and structural changes in proteins. The technique led to the discovery of unique conformational changes in HIV-1 viral capsid protein (Lanman, et. al., JMB, 325, 759-772, 2003, Cover). I became involved in many collaborative projects during my postdoctoral work at the National High Magnetic Field Lab and began to shape my career in using my analytical chemistry skillsets in high resolution tandem mass spectrometry to work with a multitude of investigators across many basic scientific and applied biomedical disciplines to understand biological functions at the molecular level. My successful multidisciplinary collaborative interaction with these investigators falls in the areas of protein identification, profiling, and quantitation; protein posttranslational modification (PTM); protein-protein interactions; natural products; metabolites; green chemistry; and organic product syntheses among many others. During my residence within the Keck MS and Proteomics Resource, I collaborated many biomedical and clinical investigators in their aims to better understand protein(s) functions in various relevant disease and cancers studies such as illustrated in Fogel et al, 2010, (which was featured on the cover of J. Biol. Chem. 285, 34864); Musante et al, 2017 (Elife 6); Miller et al, 2017 (ACS Chem Neurosci, 8(7), 1554); Sun et al, 2016 (Arthritis Rheumatology, 68(5), 1252); Tellez et al, 2013 (Sciene 341(6147), 800); Klein et al, 2017 (Neruon 95, p281); Goffredo et al, 2017 (Nutrients, 9, p 642); and Henderson et al, 2016 (Acta Neuropathol 131, p621). Currently I am involved in many projects which utilizes high resolution mass spectrometry (Orbitrap family of mass spectrometers) Label Free Quantitative LC MS and LC MS/MS methodology for protein ID/profiling and quantitation in various biological systems (i.e. brain, serum, plasma, liver, endothelial cells, FACS sorted cells, neurons, worms, mosquitos, flies, etc.), for protein ubiquitinylation, palmitoylation, acetylation, and phosphorylation modification, for small molecule quantitation and structural elucidation along with targeted drug compounds assays. The use of these exceedingly more reproducible high resolution mass spectrometry to facilitate protein and protein posttranslational modification ID and quantitative global profiling provides a very robust and biostatistical confident way to monitor protein changes, functions, and interactions within a complex biological system.

Medical Subject Headings (MeSH)

Chromatography, High Pressure Liquid; Lipids; Mass Spectrometry; Metabolism; Peptide Mapping; Peptides; Proteins; Proteomics; Research Design; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Tissue Extracts

Research at a Glance

Yale Co-Authors

Frequent collaborators of TuKiet Lam's published research.

Publications

2024

2023

Academic Achievements & Community Involvement

  • activity

    NHMFL - ICR Users Program

  • activity

    American Society for Mass Spectrometry

  • activity

    American Chemical Society

Get In Touch

Contacts

Academic Office Number
Lab Number
Mailing Address

Yale School of Medicine

Keck MS & Proteomics Resource, 300 George Street

New Haven, CT 06510

United States

Locations

  • Keck MS & Proteomics Resource

    Academic Office

    300 George Street, Fl Ground, Ste G005

    New Haven, CT 06511

    Appointments

    203.785.5086