Nikolay G Kolev, PhD

• Christian Tschudi
• Joan Steitz

Antigenic Variation; Gene Expression Regulation; Parasites; Trypanosoma; Trypanosomiasis, African; Transcriptional Activation

Disease Transmission; Infectious Diseases; Parasitology; Neglected Tropical Diseases; Trypanosomiasis; Vector-borne Diseases

• The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNAKolev NG, Rajan KS, Tycowski KT, Toh JY, Shi H, Lei Y, Michaeli S, Tschudi C. The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA. Journal Of Biological Chemistry 2019, 294: 15559-15574. PMID: 31439669, PMCID: PMC6816085, DOI: 10.1074/jbc.ra119.008580.
• Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma bruceiKolev NG, Ramsdell TK, Tschudi C. Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei. Molecular And Biochemical Parasitology 2018, 226: 20-23. PMID: 30399391, PMCID: PMC6258174, DOI: 10.1016/j.molbiopara.2018.10.003.
• The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasionChristiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Molecular Microbiology 2017, 106: 74-92. PMID: 28742275, PMCID: PMC5607103, DOI: 10.1111/mmi.13754.
• Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sitesKolev NG, Günzl A, Tschudi C. Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites. Molecular And Biochemical Parasitology 2017, 216: 52-55. PMID: 28716719, PMCID: PMC5582990, DOI: 10.1016/j.molbiopara.2017.07.002.
• The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma bruceiChikne V, Gupta SK, Doniger T, K. SR, Cohen-Chalamish S, Ben-Asher HW, Kolet L, Yahia NH, Unger R, Ullu E, Kolev NG, Tschudi C, Michaeli S. The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei. Journal Of Molecular Biology 2017, 429: 3301-3318. PMID: 28456523, DOI: 10.1016/j.jmb.2017.04.015.
• Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitansSavage AF, Kolev NG, Franklin JB, Vigneron A, Aksoy S, Tschudi C. Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitans. PLOS ONE 2016, 11: e0168877. PMID: 28002435, PMCID: PMC5176191, DOI: 10.1371/journal.pone.0168877.
• Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNAEliaz D, Doniger T, Tkacz ID, Biswas VK, Gupta SK, Kolev NG, Unger R, Ullu E, Tschudi C, Michaeli S. Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA. RNA Biology 2015, 12: 1222-1255. PMID: 25970223, PMCID: PMC4829279, DOI: 10.1080/15476286.2015.1038019.
• Synchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma bruceiRamey-Butler K, Ullu E, Kolev NG, Tschudi C. Synchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma brucei. Molecular And Biochemical Parasitology 2015, 200: 1-4. PMID: 25896436, PMCID: PMC4470760, DOI: 10.1016/j.molbiopara.2015.04.001.
• Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript EndsKolev NG, Ullu E, Tschudi C. Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends. 2014, 1201: 165-175. PMID: 25388113, DOI: 10.1007/978-1-4939-1438-8_9.
• Trypanosoma brucei RNA‐binding proteinsKolev NG, Ullu E, Tschudi C. Trypanosoma brucei RNA‐binding proteins. Cellular Microbiology 2014, 16: 482-489. PMID: 24438230, PMCID: PMC3974610, DOI: 10.1111/cmi.12268.
• Developmental Progression to Infectivity in Trypanosoma brucei Triggered by an RNA-Binding ProteinKolev NG, Ramey-Butler K, Cross GA, Ullu E, Tschudi C. Developmental Progression to Infectivity in Trypanosoma brucei Triggered by an RNA-Binding Protein. Science 2012, 338: 1352-1353. PMID: 23224556, PMCID: PMC3664091, DOI: 10.1126/science.1229641.
• Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core MachineryBarnes RL, Shi H, Kolev NG, Tschudi C, Ullu E. Comparative Genomics Reveals Two Novel RNAi Factors in Trypanosoma brucei and Provides Insight into the Core Machinery. PLOS Pathogens 2012, 8: e1002678. PMID: 22654659, PMCID: PMC3359990, DOI: 10.1371/journal.ppat.1002678.
• A single-cloning-step procedure for the generation of RNAi plasmids producing long stem–loop RNAAtayde VD, Ullu E, Kolev NG. A single-cloning-step procedure for the generation of RNAi plasmids producing long stem–loop RNA. Molecular And Biochemical Parasitology 2012, 184: 55-58. PMID: 22542486, PMCID: PMC3358476, DOI: 10.1016/j.molbiopara.2012.04.003.
• RNA Interference in Protozoan Parasites: Achievements and ChallengesKolev NG, Tschudi C, Ullu E. RNA Interference in Protozoan Parasites: Achievements and Challenges. MSphere 2011, 10: 1156-1163. PMID: 21764910, PMCID: PMC3187059, DOI: 10.1128/ec.05114-11.
• The Transcriptome of the Human Pathogen Trypanosoma brucei at Single-Nucleotide ResolutionKolev NG, Franklin JB, Carmi S, Shi H, Michaeli S, Tschudi C. The Transcriptome of the Human Pathogen Trypanosoma brucei at Single-Nucleotide Resolution. PLOS Pathogens 2010, 6: e1001090. PMID: 20838601, PMCID: PMC2936537, DOI: 10.1371/journal.ppat.1001090.
• Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma bruceiPatrick KL, Shi H, Kolev NG, Ersfeld K, Tschudi C, Ullu E. Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 17933-17938. PMID: 19815526, PMCID: PMC2764927, DOI: 10.1073/pnas.0907766106.
• snoRNAs in Giardia lamblia: a novel role in RNA silencing?Kolev NG, Ullu E. snoRNAs in Giardia lamblia: a novel role in RNA silencing? Trends In Parasitology 2009, 25: 348-350. PMID: 19616476, DOI: 10.1016/j.pt.2009.05.001.
• A manganese-dependent ribozyme in the 3′-untranslated region of Xenopus Vg1 mRNAKolev NG, Hartland EI, Huber PW. A manganese-dependent ribozyme in the 3′-untranslated region of Xenopus Vg1 mRNA. Nucleic Acids Research 2008, 36: 5530-5539. PMID: 18753150, PMCID: PMC2553595, DOI: 10.1093/nar/gkn530.
• Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3′‐end maturationKolev NG, Yario TA, Benson E, Steitz JA. Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3′‐end maturation. EMBO Reports 2008, 9: 1013-1018. PMID: 18688255, PMCID: PMC2572124, DOI: 10.1038/embor.2008.146.
• Minor-class splicing occurs in the nucleus of the Xenopus oocyteFriend K, Kolev NG, Shu MD, Steitz JA. Minor-class splicing occurs in the nucleus of the Xenopus oocyte. RNA 2008, 14: 1459-1462. PMID: 18567814, PMCID: PMC2491479, DOI: 10.1261/rna.1119708.
• In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding siteKolev NG, Steitz JA. In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site. Nature Structural & Molecular Biology 2006, 13: 347-353. PMID: 16547514, DOI: 10.1038/nsmb1075.
• Symplekin and multiple other polyadenylation factors participate in 3′-end maturation of histone mRNAsKolev NG, Steitz JA. Symplekin and multiple other polyadenylation factors participate in 3′-end maturation of histone mRNAs. Genes & Development 2005, 19: 2583-2592. PMID: 16230528, PMCID: PMC1276732, DOI: 10.1101/gad.1371105.
• VgRBP71 Stimulates Cleavage at a Polyadenylation Signal in Vg1 mRNA, Resulting in the Removal of a cis-Acting Element that Represses TranslationKolev NG, Huber PW. VgRBP71 Stimulates Cleavage at a Polyadenylation Signal in Vg1 mRNA, Resulting in the Removal of a cis-Acting Element that Represses Translation. Molecular Cell 2003, 11: 745-755. PMID: 12667456, DOI: 10.1016/s1097-2765(03)00071-6.
• Ullu, E., Kolev, N.G., Barnes, R.L. and Tschudi, C. The RNA interference pathway in Trypanosoma brucei. In RNA Metabolism in Trypanosomes, ed. Bindereif A., Series Nucleic Acids and Molecular Biology, ed. Bujnicki, J., Springer Publishing, 2012, 28:167-185.Ullu, E., Kolev, N.G., Barnes, R.L. and Tschudi, C. The RNA interference pathway in Trypanosoma brucei. In RNA Metabolism in Trypanosomes, ed. Bindereif A., Series Nucleic Acids and Molecular Biology, ed. Bujnicki, J., Springer Publishing, 2012, 28:167-185.
• Tycowski, K.T., Kolev, N.G., Conrad, N.K., Fok, V. and Steitz, J.A. The ever–growing world of small nuclear ribonucleoproteins. In The RNA World, 3rd edition, eds. Gesteland, R., Cech, T. and Atkins, J., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2006, 327-368. Tycowski, K.T., Kolev, N.G., Conrad, N.K., Fok, V. and Steitz, J.A. The ever–growing world of small nuclear ribonucleoproteins. In The RNA World, 3rd edition, eds. Gesteland, R., Cech, T. and Atkins, J., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2006, 327-368.