2023
Improvement in cardiac function and regional LV strain following intramyocardial injection of a theranostic hydrogel early postmyocardial infarction in a porcine model
Avendaño R, Midgett D, Melvinsdottir I, Thorn S, Uman S, Pickell Z, Lee S, Liu Z, Mamarian M, Duncan J, Spinale F, Burdick J, Sinusas A. Improvement in cardiac function and regional LV strain following intramyocardial injection of a theranostic hydrogel early postmyocardial infarction in a porcine model. Journal Of Applied Physiology 2023, 135: 405-420. PMID: 37318987, PMCID: PMC10538987, DOI: 10.1152/japplphysiol.00342.2022.Peer-Reviewed Original ResearchConceptsMyocardial infarctionIntramyocardial deliveryLV hemodynamicsControl groupFunctional indexEarly post-myocardial infarctionSystolic functional indicesMyocardial performance indexAnterolateral myocardial infarctionLeft anterior descendingPressure-volume loop areaLeft ventricular remodelingGlobal LV functionLV functional changesPost-myocardial infarctionSupply/demand ratioRegional LV strainHydrogel groupTei indexEjection fractionHeart failureAnterior descendingCircumflex arteryLV functionAortic pressure
2022
Cellular recovery after prolonged warm ischaemia of the whole body
Andrijevic D, Vrselja Z, Lysyy T, Zhang S, Skarica M, Spajic A, Dellal D, Thorn SL, Duckrow RB, Ma S, Duy PQ, Isiktas AU, Liang D, Li M, Kim SK, Daniele SG, Banu K, Perincheri S, Menon MC, Huttner A, Sheth KN, Gobeske KT, Tietjen GT, Zaveri HP, Latham SR, Sinusas AJ, Sestan N. Cellular recovery after prolonged warm ischaemia of the whole body. Nature 2022, 608: 405-412. PMID: 35922506, PMCID: PMC9518831, DOI: 10.1038/s41586-022-05016-1.Peer-Reviewed Original ResearchConceptsSingle-nucleus transcriptomic analysesSpecific gene expression patternsCellular recoveryGene expression patternsCellular processesMammalian cellsTranscriptomic analysisLarge mammalsExpression patternsCellular repair processesCell deathComprehensive resourceUnderappreciated potentialPhysiological challengesTissue integrityRepair processSpecific changesPorcine brainMammalsOrgansMultiple organsMatrix Metalloproteinase-Targeted SPECT/CT Imaging for Evaluation of Therapeutic Hydrogels for the Early Modulation of Post-Infarct Myocardial Remodeling
Thorn SL, Shuman JA, Stacy MR, Purcell BP, Doviak H, Burdick JA, Spinale FG, Sinusas AJ. Matrix Metalloproteinase-Targeted SPECT/CT Imaging for Evaluation of Therapeutic Hydrogels for the Early Modulation of Post-Infarct Myocardial Remodeling. Journal Of Cardiovascular Translational Research 2022, 16: 155-165. PMID: 35697979, PMCID: PMC10836411, DOI: 10.1007/s12265-022-10280-7.Peer-Reviewed Original ResearchIncreasing angular sampling through deep learning for stationary cardiac SPECT image reconstruction
Xie H, Thorn S, Chen X, Zhou B, Liu H, Liu Z, Lee S, Wang G, Liu YH, Sinusas AJ, Liu C. Increasing angular sampling through deep learning for stationary cardiac SPECT image reconstruction. Journal Of Nuclear Cardiology 2022, 30: 86-100. PMID: 35508796, DOI: 10.1007/s12350-022-02972-z.Peer-Reviewed Original ResearchConceptsDeep learningReconstruction qualityImage reconstructionDeep learning methodsDeep neural networksDeep learning resultsImage qualityNetwork trainingSPECT image reconstructionNeural networkLearning methodsHigh image resolutionImage volumesClinical softwareImage metricsImage resolutionReconstruction resultsImproved image qualityTesting dataLearning resultsNetwork resultsPhysical phantomStationary imagingDifferent subjectsLearningCineCT platform for in vivo and ex vivo measurement of 3D high resolution Lagrangian strains in the left ventricle following myocardial infarction and intramyocardial delivery of theranostic hydrogel
Midgett D, Thorn S, Ahn S, Uman S, Avendano R, Melvinsdottir I, Lysyy T, Kim J, Duncan J, Humphrey J, Papademetris X, Burdick J, Sinusas A. CineCT platform for in vivo and ex vivo measurement of 3D high resolution Lagrangian strains in the left ventricle following myocardial infarction and intramyocardial delivery of theranostic hydrogel. Journal Of Molecular And Cellular Cardiology 2022, 166: 74-90. PMID: 35227737, PMCID: PMC9035115, DOI: 10.1016/j.yjmcc.2022.02.004.Peer-Reviewed Original ResearchRegional heterogeneity in determinants of atrial matrix remodeling and association with atrial fibrillation vulnerability postmyocardial infarction
Stacy MR, Lin BA, Thorn SL, Lobb DC, Maxfield MW, Novack C, Zellars KN, Freeburg L, Akar JG, Sinusas AJ, Spinale FG. Regional heterogeneity in determinants of atrial matrix remodeling and association with atrial fibrillation vulnerability postmyocardial infarction. Heart Rhythm 2022, 19: 847-855. PMID: 35066183, PMCID: PMC9064890, DOI: 10.1016/j.hrthm.2022.01.022.Peer-Reviewed Original ResearchConceptsAtrial fibrillationMyocardial infarctionRight atrial regionsAtrial regionsPost-MIMMP activityMatrix metalloproteinasesMMP/TIMP profilesNew-onset atrial fibrillationLeft atrial regionsMMP/TIMP expressionLeft atrial appendageMatrix remodelingElectrical stimulation protocolFibrillar collagen contentExtracellular matrix remodelingAF vulnerabilityTIMP profilesAtrial appendagePostmyocardial infarctionMyocardial substrateStimulation protocolNoninvasive molecular imagingTIMP expressionTissue inhibitor
2020
Imaging of Injectable Hydrogels Delivered into Myocardium with SPECT/CT
Uman S, Wang LL, Thorn SL, Liu Z, Duncan JS, Sinusas AJ, Burdick JA. Imaging of Injectable Hydrogels Delivered into Myocardium with SPECT/CT. Advanced Healthcare Materials 2020, 9: e2000294. PMID: 32543053, PMCID: PMC7482444, DOI: 10.1002/adhm.202000294.Peer-Reviewed Original ResearchConceptsInjectable hydrogelsMyocardial infarctionSelf-healing hydrogelsHydrogel mechanical propertiesHybrid single-photon emissionHybrid SPECT/CT imagingMechanical propertiesTomography perfusion imagingAcute porcine modelSPECT/CT imagingSPECT/CTSingle photon emissionInjection forceHyaluronic acid hydrogelsPattern of deliveryTomography/HydrogelsPerfusion defectsPerfusion imagingExcellent radiopacityPorcine modelAcid hydrogelsCT imagingHydrogel deliveryHydrogel injection
2019
Application of Hybrid Matrix Metalloproteinase-Targeted and Dynamic 201Tl Single-Photon Emission Computed Tomography/Computed Tomography Imaging for Evaluation of Early Post-Myocardial Infarction Remodeling
Thorn SL, Barlow SC, Feher A, Stacy MR, Doviak H, Jacobs J, Zellars K, Renaud JM, Klein R, deKemp RA, Khakoo AY, Lee T, Spinale FG, Sinusas AJ. Application of Hybrid Matrix Metalloproteinase-Targeted and Dynamic 201Tl Single-Photon Emission Computed Tomography/Computed Tomography Imaging for Evaluation of Early Post-Myocardial Infarction Remodeling. Circulation Cardiovascular Imaging 2019, 12: e009055. PMID: 31707811, PMCID: PMC7250243, DOI: 10.1161/circimaging.119.009055.Peer-Reviewed Original ResearchConceptsMyocardial blood flowSingle photon emissionBlood flowRecombinant TIMP-3Matrix metalloproteinasesR injuryTomography/Intracoronary deliveryMMP activationPost-myocardial infarction remodelingSubsequent left ventricular remodelingHybrid single-photon emissionDual-isotope single-photon emissionTomography/Computed Tomography ImagingLeft ventricular remodelingIschemia/reperfusionPost-MI remodelingPost-myocardial infarctionComputed tomography (CT) imagingIntracoronary infusionVentricular functionSaline groupVentricular remodelingNoninvasive evaluationTreatment groupsIncisional Negative Pressure Wound Therapy Augments Perfusion and Improves Wound Healing in a Swine Model Pilot Study.
Shah A, Sumpio BJ, Tsay C, Swallow M, Dash B, Thorn SL, Sinusas AJ, Koo A, Hsia HC, Au A. Incisional Negative Pressure Wound Therapy Augments Perfusion and Improves Wound Healing in a Swine Model Pilot Study. Annals Of Plastic Surgery 2019, 82: s222-s227. PMID: 30855392, DOI: 10.1097/sap.0000000000001842.Peer-Reviewed Original ResearchConceptsNegative pressure wound therapyVascular endothelial growth factorClosed woundsUse of NPWTVEGF expressionSurgical wound sitePressure wound therapyMale Yorkshire pigsEnzyme-linked immunosorbent assay resultsEnzyme-linked immunosorbent assayEndothelial growth factorExcisional wound modelImmunosorbent assay resultsControl dressingsPrimary sutureScar evaluationImmunohistochemistry stainingIncision siteNPWT dressingsWound therapyAngiogenesis markersTreatment groupsAppearance of woundsOpen woundsWound healing model
2013
Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium
Giordano C, Thorn SL, Renaud JM, Al-Atassi T, Boodhwani M, Klein R, Kuraitis D, Dwivedi G, Zhang P, DaSilva JN, Ascah KJ, deKemp RA, Suuronen EJ, Beanlands RS, Ruel M. Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium. Circulation Cardiovascular Imaging 2013, 6: 982-991. PMID: 24089461, DOI: 10.1161/circimaging.113.000185.Peer-Reviewed Original ResearchConceptsMyocardial blood flowHibernating myocardiumAngiogenic cellsBlood flowSwine modelAdvanced coronary artery diseaseLeft circumflex artery territoryRelevant swine modelCoronary artery diseaseCircumflex artery territoryLeft circumflex arteryCirculating Angiogenic CellsWall motion abnormalitiesMyocardial flow reserveArtery territoryUnderwent placementArtery diseaseEjection fractionCircumflex arteryCell-based therapiesRevascularization approachTomography perfusionMyocardial hibernationAmeroid constrictorPreclinical swine model