2023
Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal Cancer
Cecchini M, Zhang J, Wei W, Sklar J, Lacy J, Zhong M, Kong Y, Zhao H, DiPalermo J, Devine L, Stein S, Kortmansky J, Johung K, Bindra R, LoRusso P, Schalper K. Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal Cancer. Cancer Research Communications 2023, 3: 1132-1139. PMID: 37387791, PMCID: PMC10305782, DOI: 10.1158/2767-9764.crc-23-0045.Peer-Reviewed Original ResearchConceptsWhole-exome sequencingMGMT protein expressionColorectal cancerStable diseaseQuantitative immunofluorescenceT cellsProtein expressionPromoter hypermethylationLow MGMT protein expressionPARP inhibitorsRadiographic tumor regressionMetastatic colorectal cancerAdvanced colorectal cancerPretreatment tumor biopsiesEffector T cellsTumor-infiltrating lymphocytesMGMT proteinDNA repair biomarkersBaseline CD8Eligible patientsIncreased CD8Methylguanine-DNA methyltransferaseObjective responseProgressive diseaseImmune markers
2022
Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults
Chou C, Mohanty S, Kang HA, Kong L, Avila‐Pacheco J, Joshi SR, Ueda I, Devine L, Raddassi K, Pierce K, Jeanfavre S, Bullock K, Meng H, Clish C, Santori FR, Shaw AC, Xavier RJ. Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults. Aging Cell 2022, 21: e13682. PMID: 35996998, PMCID: PMC9470889, DOI: 10.1111/acel.13682.Peer-Reviewed Original ResearchConceptsInfluenza vaccine responsesInfluenza vaccinationVaccine responsesHigh respondersAntibody responseImmune responseMore effective influenza vaccinesOlder adultsEffective influenza vaccinesSevere respiratory infectionsRobust immune responseLow antibody responseInfluenza vaccineRespiratory infectionsSignificant morbiditySeasonal influenzaInflammatory responseAge-related differencesDay 28Flu seasonOlder subjectsVaccinationHR subjectsMetabolomic signaturePlasma metabolites
2018
Elevated Levels of CD64 MFI on Monocyte Subsets Are Associated with a History of Stroke in Sickle Cell Disease
Curtis S, Balbuena-Merle R, Devine L, Zelterman D, Roberts J, Dearborn-Tomazos J, Sansing L, Hendrickson J. Elevated Levels of CD64 MFI on Monocyte Subsets Are Associated with a History of Stroke in Sickle Cell Disease. Blood 2018, 132: 1093. DOI: 10.1182/blood-2018-99-117504.Peer-Reviewed Original ResearchHistory of strokeSickle cell diseaseRole of monocytesIschemic strokeTotal neutrophil countComplete blood countMonocyte subsetsStroke historyNeutrophil countIntermediate monocytesMonocyte subtypesBlood countClassical monocytesCell diseaseFuture stroke riskIschemic stroke historyVaso-occlusive crisisPro-inflammatory monocytesPathophysiology of strokeCause of morbidityMultivariate logistic regressionNon-classical monocytesT-testHigh-affinity receptorStudent's t-test
2008
Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by Ubiquitination
Thakral D, Dobbins J, Devine L, Kavathas PB. Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by Ubiquitination. The Journal Of Immunology 2008, 180: 7431-7442. PMID: 18490743, DOI: 10.4049/jimmunol.180.11.7431.Peer-Reviewed Original ResearchConceptsCytoplasmic tailSplice variantsExpression patternsCell surfaceDifferent cytoplasmic tailsGene splice variantsMRNA expression patternsMRNA levelsFluorescent chimerasHuman genesHuman lineageDifferential mRNA expression patternsHeterodimer functionsExtracellular domainLysine residuesQuantitative RT-PCRDifferential expressionLysosomal compartmentTCR complexFunctional significanceIsoformsCD8B geneUbiquitinationCell linesPrimary humanDifferential Expression of Human CD8 Beta‐chain Isoforms and Regulation of M‐2 Isoform (splice variant 1) by Ubiquitination
Thakral D, Dobbins J, Devine L, Kavathas P. Differential Expression of Human CD8 Beta‐chain Isoforms and Regulation of M‐2 Isoform (splice variant 1) by Ubiquitination. The FASEB Journal 2008, 22: 367-367. DOI: 10.1096/fasebj.22.2_supplement.367.Peer-Reviewed Original ResearchCytoplasmic tailCell surfaceDifferent cytoplasmic tailsMRNA expression patternsMurine geneMRNA levelsFluorescent chimerasDifferential mRNA expression patternsExpression patternsExtracellular domainQuantitative RT-PCRDifferential expressionLysine residuesDifferential regulationUbiquitinationLysosomal compartmentSplice variantsTCR complexIsoformsT cell receptorPrimary human CD8CD8B geneMRNA patternsCell linesGenes
2006
Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding
Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB. Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding. The Journal Of Immunology 2006, 177: 3930-3938. PMID: 16951356, DOI: 10.4049/jimmunol.177.6.3930.Peer-Reviewed Original ResearchConceptsMHC class IClass IT cellsT cell responsesEffective immune responseT cell recognitionT cell hybridomasT cell membraneImmune responseCoreceptor functionCell responsesCell hybridomasInfected cellsCell recognitionIdentified mutationsCD8alphaCoreceptorCD8betaCDR3 loopsCellsEnhanced bindingCD8ImmunotherapyCD8alphabetaCD8β
2004
Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation
Devine L, Hodsdon ME, Daniels MA, Jameson SC, Kavathas PB. Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation. Immunology Letters 2004, 93: 123-130. PMID: 15158607, DOI: 10.1016/j.imlet.2004.02.002.Peer-Reviewed Original ResearchAnimalsAntibodies, MonoclonalAntibody AffinityAntibody SpecificityBiotinylationCD8 AntigensChlorocebus aethiopsCOS CellsEgg ProteinsEpitope MappingEscherichia coliFlow CytometryH-2 AntigensHistocompatibility Antigens Class IMiceModels, MolecularMutagenesis, Site-DirectedOligopeptidesOvalbuminPeptide FragmentsProtein BindingProtein ConformationTransfection
2002
The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 Domains
Devine L, Rogozinski L, Naidenko OV, Cheroutre H, Kavathas PB. The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 Domains. The Journal Of Immunology 2002, 168: 3881-3886. PMID: 11937542, DOI: 10.4049/jimmunol.168.8.3881.Peer-Reviewed Original ResearchAmino Acid SequenceAmino Acid SubstitutionAnimalsAntigens, NeoplasmBeta 2-MicroglobulinCD8 AntigensComplementarity Determining RegionsCOS CellsDimerizationH-2 AntigensImmune SeraMembrane GlycoproteinsMiceMolecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsProtein BindingProtein Structure, SecondaryProtein Structure, TertiaryThymus GlandTransfectionA Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8
Campbell N, Park M, Toy L, Yio X, Devine L, Kavathas P, Mayer L. A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8. Clinical Immunology 2002, 102: 267-274. PMID: 11890713, DOI: 10.1006/clim.2001.5170.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAdaptor Proteins, Signal TransducingAnti-Bacterial AgentsAntibodies, MonoclonalBlotting, WesternCarcinoma, HepatocellularCarrier ProteinsCD40 AntigensCD8-Positive T-LymphocytesEnterocytesEnzyme-Linked Immunosorbent AssayEpitopesExtracellular Matrix ProteinsHumansImmediate-Early ProteinsLiver NeoplasmsMembrane GlycoproteinsPhosphorylationProteinsRecombinant Fusion ProteinsSequestosome-1 ProteinTransfectionTumor Cells, CulturedTunicamycinConceptsActivation of CD8T cellsIntestinal epithelial cellsCD8 moleculesEpithelial cellsMucosal immune responsesClass I MHCNormal intestinal epithelial cellsT cell activationMurine T cellsCD8 alphaImmune responseCytolytic activityI MHCCell activationMixed cell culture systemCD8Human CD8 alphaMonoclonal antibodiesCell culture systemActivationSurface glycoproteinTyrosine kinaseCellsGp180
2001
CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and Glycosylation
Daniels M, Devine L, Miller J, Moser J, Lukacher A, Altman J, Kavathas P, Hogquist K, Jameson S. CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and Glycosylation. Immunity 2001, 15: 1051-1061. PMID: 11754824, DOI: 10.1016/s1074-7613(01)00252-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersCD3 ComplexCD4-Positive T-LymphocytesCD8 AntigensCD8-Positive T-LymphocytesCell AdhesionCell DifferentiationCellular SenescenceGlycosylationH-2 AntigensHistocompatibility Antigen H-2DLigandsMacromolecular SubstancesMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutN-Acetylneuraminic AcidNeuraminidaseOvalbuminPeptide FragmentsProtein BindingProtein Processing, Post-TranslationalReceptors, Antigen, T-Cell, alpha-betaRheologySolubilityThymus GlandT-Lymphocyte SubsetsConceptsGlycosylation stateT cell maturationCell maturationT cell adhesionDevelopmental regulationDouble-positive thymocytesCell adhesionT cell activationClass I MHC tetramersAdhesion moleculesCell activationMHC class I moleculesMaturationClass I moleculesClass I MHC moleculesI MHC moleculesI moleculesMoleculesGlycosylationMHC ligandsRegulationMHC moleculesClass I MHCBindingSialylation
2000
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
Kim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsCells, CulturedChlamydia trachomatisCytotoxicity Tests, ImmunologicEpitopes, T-LymphocyteFemaleHematopoietic Stem CellsHLA-A2 AntigenHLA-B AntigensHumansImmunomagnetic SeparationLymphocyte CountLymphogranuloma VenereumMalePorinsProtein BindingT-Lymphocytes, CytotoxicConceptsHLA class ITetramer-binding T cellsCTL responsesT cellsPeripheral bloodClass IPeptide-specific T cellsHLA-A2 tetramersPeptide-pulsed targetsGenital tract infectionElicit CTL responsesMajor outer membrane proteinTetramer analysisTract infectionsPeptide stimulationImmunization trialsCytolytic activityChlamydia trachomatisUninfected individualsVivo inductionInfected individualsEpithelial cellsBloodBulk culturesStimulationHuman CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer
Devine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.Peer-Reviewed Original ResearchConceptsChimeric proteinIg domainsCell-cell adhesion assaysBeta-beta dimersCD8 alphaForms of CD8CD8 betaTyrosine kinase p56lckCOS-7 cellsMHC class IDisulfide-linked homodimerCD8 alpha alpha homodimerCysteine residuesKinase p56lckCD8 alpha alphaAlpha alpha homodimerLack of expressionAlpha polypeptide chainPolypeptide chainMature T cellsCell surfaceClass IBeta-IgBeta betaAbility of CD8
1999
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8
Devine L, Kavathas P. Molecular analysis of protein interactions mediating the function of the cell surface protein CD8. Immunologic Research 1999, 19: 201-210. PMID: 10493174, DOI: 10.1007/bf02786488.Peer-Reviewed Original ResearchConceptsProtein interactionsForms of CD8Such protein interactionsTyrosine kinase p56lckDisulfide-linked homodimerCell surface glycoproteinMolecular detailsKinase p56lckMutational analysisMolecular analysisFunctional differencesCoreceptor CD8ProteinHistocompatibility complex class IAffinity measurementsMajor histocompatibility complex class IComplex class ICellsP56lckHomodimerHeterodimersCrystal structureClass IGlycoproteinInteractionOrientation of the Ig domains of CD8 alpha beta relative to MHC class I.
Devine L, Sun J, Barr M, Kavathas P. Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. The Journal Of Immunology 1999, 162: 846-51. PMID: 9916707, DOI: 10.4049/jimmunol.162.2.846.Peer-Reviewed Original Research
1996
SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics
Greenwood J, Pryce G, Devine L, Male D, dos Santos W, Calder V, Adamson P. SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal Of Neuroimmunology 1996, 71: 51-63. PMID: 8982103, DOI: 10.1016/s0165-5728(96)00130-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Polyomavirus TransformingAntigens, SurfaceBiological TransportBlood-Brain BarrierCell Adhesion MoleculesCell MovementCell Transformation, ViralEndotheliumEndothelium, VascularFluorescent Antibody Technique, IndirectHistocompatibility AntigensLipoproteins, LDLPigment Epithelium of EyeRatsRetinaSimian virus 40T-LymphocytesConceptsRetinal pigment epitheliumBlood-retinal barrierRetinal endotheliumAdhesion moleculesCell adhesion moleculeCell linesAntigen-specific T cell linesImmortalised cell linesMajor histocompatibility complex class 1Vascular cell adhesion moleculeRat brain endotheliumMHC class IIEndothelial cell adhesion moleculesIntercellular adhesion moleculeCentral nervous systemLectin Griffonia simplicifoliaPlatelet endothelial cell adhesion moleculeHigh-affinity uptakeT cell linesPigmented epithelial cellsRPE cell lineVon Willebrand factorEndothelial cell lineNeural parenchymaBrain endotheliumRole of LFA‐1, ICAM‐1, VLA‐4 and VCAM‐1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro
DEVINE L, LIGHTMAN S, GREENWOOD J. Role of LFA‐1, ICAM‐1, VLA‐4 and VCAM‐1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology 1996, 88: 456-462. PMID: 8774365, PMCID: PMC1456340, DOI: 10.1046/j.1365-2567.1996.d01-666.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood-Retinal BarrierCD4-Positive T-LymphocytesCell Adhesion MoleculesCell Culture TechniquesCell MovementChemotaxis, LeukocyteEnzyme-Linked Immunosorbent AssayFlow CytometryIntercellular Adhesion Molecule-1Microscopy, ElectronPigment Epithelium of EyeRatsRats, Inbred StrainsVascular Cell Adhesion Molecule-1ConceptsBlood-retinal barrierRetinal pigment epitheliumLFA-1 betaICAM-1VCAM-1VLA-4Vascular endotheliumLymphocyte migrationAntigen-specific T cell linesAdhesion moleculesRetinal vascular endotheliumMigration of CD4Traffic of lymphocytesOcular inflammatory conditionsLFA-1 alphaVCAM-1 expressionRetinal pigment epithelial monolayerRPE cell monolayersT cell linesPosterior uveitisLymphocyte trafficInflammatory conditionsPosterior barrierCell adhesion moleculeIFN-gammaLymphocyte Migration across the Anterior and Posterior Blood–Retinal Barrierin Vitro
Devine L, Lightman S, Greenwood J. Lymphocyte Migration across the Anterior and Posterior Blood–Retinal Barrierin Vitro. Cellular Immunology 1996, 168: 267-275. PMID: 8640874, DOI: 10.1006/cimm.1996.0075.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigensArrestinBlood-Retinal BarrierCell AdhesionCell LineChemotaxis, LeukocyteEndothelium, VascularEye ProteinsInterferon-gammaInterleukin-1Lymph NodesLymphocyte ActivationLymphocyte SubsetsMuromonab-CD3OvalbuminPigment Epithelium of EyeRatsRats, Inbred LewRats, Inbred StrainsReceptor-CD3 Complex, Antigen, T-CellRecombinant ProteinsRetinal VesselsSpecific Pathogen-Free OrganismsT-Lymphocytes, Helper-InducerTuberculinConceptsAntigen-specific T cell linesRat retinal pigment epitheliumBlood-retinal barrierT cell linesPLN cellsEndothelial cellsLymphocyte migrationPeripheral lymph node cellsCell linesRetinal vascular endotheliumLymph node cellsCross-linking CD3Migration of lymphocytesRetinal endothelial cellsRetinal pigment epitheliumCombination of CD3PVG ratsRetinal endotheliumIFN-gamma activationLewis ratsNode cellsS-antigenICAM-1IFN-gammaPigment epitheliumA Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in Vitro
Devine L, Lightman S, Greenwood J. A Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in Vitro. Advances In Behavioral Biology 1996, 46: 245-251. DOI: 10.1007/978-1-4757-9489-2_41.Peer-Reviewed Original ResearchBlood-retinal barrierCentral nervous systemRetinal pigment epitheliumImmune mediated diseasesSystemic immune systemVLA-4/VCAMTraffic of lymphocytesLFA-1/ICAMBlood-tissue barriersMediated diseasesCytokine activationLymphocyte migrationVascular endotheliumPigment epitheliumNervous systemImmune systemRPE monolayerAdhesion moleculesLymphocytesVascular endothelialReceptor-ligand pairingsCellular barriersEyesSeparate sitesVCAM
1995
The Blood-Retinal Barrier in Immune-Mediated Diseases of the Retina
Greenwood J, Bamforth S, Wang Y, Devine L. The Blood-Retinal Barrier in Immune-Mediated Diseases of the Retina. 1995, 315-326. DOI: 10.1007/978-1-4899-1054-7_31.Peer-Reviewed Original ResearchBlood-brain barrierBlood-retinal barrierBlood-CSF barrierCentral nervous systemTight junctionsBlood-tissue barriersFenestrated vasculatureCerebral parenchymaCerebral vasculatureChoroid plexusNervous systemEndothelial cellsChoroidal epitheliumExtracellular fluidDelicate homeostasisRetinaSystemic influencesBrainVasculatureCellular barriersPlexusNeuroretinaDiseaseBloodParenchyma