2004
Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation
Devine L, Hodsdon ME, Daniels MA, Jameson SC, Kavathas PB. Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation. Immunology Letters 2004, 93: 123-130. PMID: 15158607, DOI: 10.1016/j.imlet.2004.02.002.Peer-Reviewed Original ResearchAnimalsAntibodies, MonoclonalAntibody AffinityAntibody SpecificityBiotinylationCD8 AntigensChlorocebus aethiopsCOS CellsEgg ProteinsEpitope MappingEscherichia coliFlow CytometryH-2 AntigensHistocompatibility Antigens Class IMiceModels, MolecularMutagenesis, Site-DirectedOligopeptidesOvalbuminPeptide FragmentsProtein BindingProtein ConformationTransfection
2002
The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 Domains
Devine L, Rogozinski L, Naidenko OV, Cheroutre H, Kavathas PB. The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 Domains. The Journal Of Immunology 2002, 168: 3881-3886. PMID: 11937542, DOI: 10.4049/jimmunol.168.8.3881.Peer-Reviewed Original ResearchAmino Acid SequenceAmino Acid SubstitutionAnimalsAntigens, NeoplasmBeta 2-MicroglobulinCD8 AntigensComplementarity Determining RegionsCOS CellsDimerizationH-2 AntigensImmune SeraMembrane GlycoproteinsMiceMolecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsProtein BindingProtein Structure, SecondaryProtein Structure, TertiaryThymus GlandTransfection
2001
CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and Glycosylation
Daniels M, Devine L, Miller J, Moser J, Lukacher A, Altman J, Kavathas P, Hogquist K, Jameson S. CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and Glycosylation. Immunity 2001, 15: 1051-1061. PMID: 11754824, DOI: 10.1016/s1074-7613(01)00252-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersCD3 ComplexCD4-Positive T-LymphocytesCD8 AntigensCD8-Positive T-LymphocytesCell AdhesionCell DifferentiationCellular SenescenceGlycosylationH-2 AntigensHistocompatibility Antigen H-2DLigandsMacromolecular SubstancesMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutN-Acetylneuraminic AcidNeuraminidaseOvalbuminPeptide FragmentsProtein BindingProtein Processing, Post-TranslationalReceptors, Antigen, T-Cell, alpha-betaRheologySolubilityThymus GlandT-Lymphocyte SubsetsConceptsGlycosylation stateT cell maturationCell maturationT cell adhesionDevelopmental regulationDouble-positive thymocytesCell adhesionT cell activationClass I MHC tetramersAdhesion moleculesCell activationMHC class I moleculesMaturationClass I moleculesClass I MHC moleculesI MHC moleculesI moleculesMoleculesGlycosylationMHC ligandsRegulationMHC moleculesClass I MHCBindingSialylation
2000
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
Kim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsCells, CulturedChlamydia trachomatisCytotoxicity Tests, ImmunologicEpitopes, T-LymphocyteFemaleHematopoietic Stem CellsHLA-A2 AntigenHLA-B AntigensHumansImmunomagnetic SeparationLymphocyte CountLymphogranuloma VenereumMalePorinsProtein BindingT-Lymphocytes, CytotoxicConceptsHLA class ITetramer-binding T cellsCTL responsesT cellsPeripheral bloodClass IPeptide-specific T cellsHLA-A2 tetramersPeptide-pulsed targetsGenital tract infectionElicit CTL responsesMajor outer membrane proteinTetramer analysisTract infectionsPeptide stimulationImmunization trialsCytolytic activityChlamydia trachomatisUninfected individualsVivo inductionInfected individualsEpithelial cellsBloodBulk culturesStimulationHuman CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer
Devine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.Peer-Reviewed Original ResearchConceptsChimeric proteinIg domainsCell-cell adhesion assaysBeta-beta dimersCD8 alphaForms of CD8CD8 betaTyrosine kinase p56lckCOS-7 cellsMHC class IDisulfide-linked homodimerCD8 alpha alpha homodimerCysteine residuesKinase p56lckCD8 alpha alphaAlpha alpha homodimerLack of expressionAlpha polypeptide chainPolypeptide chainMature T cellsCell surfaceClass IBeta-IgBeta betaAbility of CD8