2019
Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold
Braddock D, Stabach P, Zimmerman K, Kavanagh D, Sauei C, Yarema K. Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold. The FASEB Journal 2019, 33: 801.1-801.1. DOI: 10.1096/fasebj.2019.33.1_supplement.801.1.Peer-Reviewed Original ResearchFusion proteinProtein engineeringCHO cell linesCellular recyclingBioprocessing methodsRecombinant fusion proteinConsensus sequenceExtracellular domainSialic acidTertiary structureN-acetylmannosamineCHO cellsHuman alphaProteinCell linesFASEB JournalFc domainCell culture mediumCulture mediumGeneralized Arterial CalcificationENPP1
2016
Novel Biologic Therapy for Untreated Diseases of Vascular Calcification
Braddock D, Kavanagh D, Li X. Novel Biologic Therapy for Untreated Diseases of Vascular Calcification. Blood 2016, 128: 2326. DOI: 10.1182/blood.v128.22.2326.2326.Peer-Reviewed Original ResearchConnective tissue diseasePlasma PPiTissue calcificationPseudoxanthoma elasticumABCC6 mutationsTissue diseaseVascular calcificationMembrane transport proteinsKnockout miceStem cell progenitorsMineralization inhibitorPotent mineralization inhibitorNovel biologic therapiesElastic fiber alterationsTransport proteinsFusion proteinVivo experimental evidenceCell progenitorsFunction mutationsBiologic therapyUntreated diseaseArterial calcificationNeonatal diseaseSpecific tissuesCommon disorder