GFP
4D movie of endocytosis (transferrin cargo in red, clathrin coated pits and vesicles in green)
To create a state-of-the-art light microscopes imaging center. This will provide cutting edge technologies to address complex cell biological questions that can not be addressed with standard methodologies.
For instance, Total Internal Reflection Fluorescent Microscopy (TIRFM) allows imaging of a very thin plane (<100 nm) so events that occur near the cell surface (e.g. membrane trafficking, cytoskeleton) can be observed with exquisite clarity. With this approach detection of single molecules are possible. A 4D (3D + time) multicolor spinning disk confocal microscopy (SDCM) allows for fast live cell imaging of fast moving dim objects that traverse several focal planes.
The CINEMA Lab imaging center is located in SHM IE17/19/21 and is adjacent to the CCMI facility and EM facilites (IE-wing, basement of SHM). CINEMA Lab is equipped to accommodate 6 advanced microscope setups. It was can accommodate water-cooled lasers (20-70 Amps) and multiple air-cooled lasers and has ceiling equipment racks and blackout curtains. It is designed for use with research grade lasers in safety: the room is electronically card coded, has emergency cut-off switches and warning signs, curtained partitions, and water leak detection.
It also has an adjacent tissue cell culture room with CO2 incubators, two class II biosafety hood, a refrigerator/freezer, and centrifuges. Thus researchers can easily prepare samples in advance without worrying about damaging cells in transit and use sterile technique when preparing cells or working with viruses. There is also a ‘wet-lab’ bench so that researchers can easily manipulate samples before or during use. Last, the center has a bench with Ethernet links to accommodate post-data acquisition computers.
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TIRFM: Multicolor Total Internal Reflection Fluorescent Microscope (TIRFM) using an inverted Olympus microscope (IX-70 and IX-71) base and custom TIRFM illumination optics. Prism-type upright TIRFM based on an upright Olympus microscope (BX-51).
TIRFM: Multicolor Total Internal Reflection Fluorescent Microscope (TIRFM) using an inverted Olympus microscope (IX-70 and IX-71) base and custom TIRFM illumination optics. Prism-type upright TIRFM based on an upright Olympus microscope (BX-51).
Leica STED Super-Resolution Microscope (Contact: Joerg Bewersdorf)
Multicolor spinning-disk confocal based on an inverted Olympus microscope (IX-71) and Perkin-Elmer Ultraview system with 5-laser and FRAP/photoactivation.
Our mission is to provide electron microscopy services and techniques to investigators at Yale School of Medicine.
We offer a wide range of services from conventional EM to cryosectioning and immunogold labeling.
To learn more about CCMI click here.