2024
Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex
Chavali S, Chou S, Cao W, Pollard T, De La Cruz E, Sindelar C. Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex. Nature Communications 2024, 15: 2059. PMID: 38448439, PMCID: PMC10918085, DOI: 10.1038/s41467-024-46179-x.Peer-Reviewed Original ResearchConceptsArp2/3 complexActin filamentsCryo-EM structureMother filamentDaughter filamentArp2/3 complex nucleates branched actin filamentsActin filament branchingBranched actin filamentsDissociation of PiADP-PiFilament branchingOrganelle movementADP stateBranch junctionsArp3A-resolutionActinArp2/3ADP-BeFxFilamentsADPPhosphate releaseFilament mechanismArp2Organelles
2020
Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches
Pandit NG, Cao W, Bibeau J, Johnson-Chavarria EM, Taylor EW, Pollard TD, De La Cruz EM. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 13519-13528. PMID: 32461373, PMCID: PMC7306818, DOI: 10.1073/pnas.1911183117.Peer-Reviewed Original ResearchConceptsActin filament branchesArp2/3 complexMother filamentFilament branchesTotal internal reflection fluorescence microscopyEssential cellular functionsMechanical forcesActin filament networkReflection fluorescence microscopyCellular functionsActin networkCell motilityComplex generatesActin filamentsArp2/3Filament networkFluorescence microscopyState 1Branch junctionsState 2FilamentsComplexesPhosphate releaseMuscle actinADP
2013
Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches
Chen Q, Pollard TD. Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches. Current Biology 2013, 23: 1154-1162. PMID: 23727096, PMCID: PMC4131202, DOI: 10.1016/j.cub.2013.05.005.Peer-Reviewed Original ResearchConceptsSites of endocytosisActin patchesWild-type cellsArp2/3 complexMother filamentActin filamentsAdaptor proteinActin polymerizationYeast cellsBranched actin filament networksEndocytic adaptor proteinActivators of Arp2/3Fission yeast cellsActin filament severingActin filament networkNew patchesEndocytic sitesQuantitative fluorescence microscopyMutant cofilinsMutant cellsNascent sitesFilament severingCofilinFilament networkEndocytosis
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply