2017
Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers
Zhang Y. Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers. Protein Science 2017, 26: 1252-1265. PMID: 28097727, PMCID: PMC5477538, DOI: 10.1002/pro.3116.Peer-Reviewed Original ResearchConceptsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsSynaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptorSNARE assemblyMembrane fusionSNARE complexN-ethylmaleimide-sensitive factor attachment protein receptorsFactor attachment protein receptorsFast calcium-triggered fusionAttachment protein receptorsHigh-resolution optical tweezersCalcium-triggered fusionC-terminal domainFour-helix bundleNeurotransmitter-containing vesiclesLinker domainPlasma membraneDomain associationProtein receptorsMolecular engineDifferent functionsPathwayAssemblyMembraneOptical tweezersSynaptic transmission
2016
α-SNAP Enhances SNARE Zippering by Stabilizing the SNARE Four-Helix Bundle
Ma L, Kang Y, Jiao J, Rebane AA, Keun HK, Xi Z, Qu H, Zhang Y. α-SNAP Enhances SNARE Zippering by Stabilizing the SNARE Four-Helix Bundle. Cell Reports 2016, 15: 531-539. PMID: 27068468, PMCID: PMC4838522, DOI: 10.1016/j.celrep.2016.03.050.Peer-Reviewed Original ResearchConceptsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsC-terminal domainN-terminal domainMembrane fusionLinker domainΑ-SNAPSynaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptorN-ethylmaleimide-sensitive factor attachment protein receptorsFactor attachment protein receptorsIntracellular membrane fusionAttachment protein receptorsConformational selection mechanismFour-helix bundleSNARE assemblySNARE complexProtein receptorsDynamic assemblyStepwise assemblyAssemblyDomainFusionZipperingOptical tweezersRecent experimentsReceptors
2015
Munc18-1-regulated stage-wise SNARE assembly underlying synaptic exocytosis
Ma L, Rebane AA, Yang G, Xi Z, Kang Y, Gao Y, Zhang Y. Munc18-1-regulated stage-wise SNARE assembly underlying synaptic exocytosis. ELife 2015, 4: e09580. PMID: 26701912, PMCID: PMC4744192, DOI: 10.7554/elife.09580.Peer-Reviewed Original ResearchConceptsN-terminal regulatory domainSNARE assemblyMunc18-1Folding/assemblySNARE domainVesicle dockingDifferent assembly stagesRegulatory domainLinker domainSynaptic exocytosisDistinct functionsRapid exocytosisC-terminalN-terminalExocytosisAttachment receptorAssemblyDistinct stagesSyntaxinDomainMutationsFusionOptical tweezersAssembly stageDocking
2013
Oligomerization and higher‐order assembly contribute to sub‐cellular localization of a bacterial scaffold
Bowman GR, Perez AM, Ptacin JL, Ighodaro E, Folta‐Stogniew E, Comolli LR, Shapiro L. Oligomerization and higher‐order assembly contribute to sub‐cellular localization of a bacterial scaffold. Molecular Microbiology 2013, 90: 776-795. PMID: 24102805, PMCID: PMC3859194, DOI: 10.1111/mmi.12398.Peer-Reviewed Original ResearchConceptsSub-cellular localizationAmino acidsN-terminal 23 amino acidsDefective mutant proteinsAsymmetric cell divisionC-terminal 76 amino acidsC-terminal domainDimer of trimersCaulobacter crescentusCell polesLocalization determinantsHigher-order structureMutant proteinsScaffold proteinSubcellular fociCell divisionLinker domainPopZMutational analysisBiophysical analysisWild typePolar organizationOrganizing centerDistinct setsProtein
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