2001
Small Marker Chromosome Identification in Metaphase and Interphase Using Centromeric Multiplex FISH (CM-FISH)
Henegariu O, Bray-Ward P, Artan S, Vance G, Qumsyieh M, Ward D. Small Marker Chromosome Identification in Metaphase and Interphase Using Centromeric Multiplex FISH (CM-FISH). Laboratory Investigation 2001, 81: 475-481. PMID: 11304566, DOI: 10.1038/labinvest.3780255.Peer-Reviewed Original ResearchConceptsHuman chromosome painting probesMolecular cytogenetic proceduresDNA probesSubtelomeric chromosomal regionsIdentification of aneuploidyChromosome painting probesM-FISH analysisAlpha-satellite regionMarker chromosome identificationMarker chromosomesChromosome identificationHuman chromosomesRepetitive sequencesChromosomal rearrangementsInterphase nucleiMultiplex FISHChromosomal regionsPainting probesMetaphase assayMultiplex fluorescenceM-FISHCytogenetic proceduresSpectral karyotypingChromosomesPlasmid probes
1992
Cytostellin: a novel, highly conserved protein that undergoes continuous redistribution during the cell cycle
Warren S, Landolfi A, Curtis C, Morrow J. Cytostellin: a novel, highly conserved protein that undergoes continuous redistribution during the cell cycle. Journal Of Cell Science 1992, 103: 381-388. PMID: 1478941, DOI: 10.1242/jcs.103.2.381.Peer-Reviewed Original ResearchConceptsMitotic spindle apparatusSpindle apparatusLower eukaryotic cellsOnset of prophaseEukaryotic cellsDaughter cellsCytoplasmic proteinsMammalian cellsMonoclonal antibody H5Interphase nucleiTelophase cellsNuclease digestionCell cycleImmunofluorescence microscopyImmunoblot analysisCell processesProteinNascent nucleiSalt extractionImmunoaffinity chromatographyCellsDistinct subsetsNucleusAnaphaseProphase
1988
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries
Lichter P, Cremer T, Borden J, Manuelidis L, Ward D. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988, 80: 224-234. PMID: 3192212, DOI: 10.1007/bf01790090.Peer-Reviewed Original ResearchConceptsIndividual human chromosomesHuman chromosomesDNA insertsInterphase nucleiInterphase cellsTotal human genomic DNASitu suppression hybridizationPost-hybridization washesRecombinant DNA libraryHuman genomic DNAChromosomal domainsConfocal fluorescence microscopyChromosomal librarySuppression hybridizationDNA librarySingle-strand DNARepetitive sequencesCognate chromosomeChromosome 1Genomic DNAChromosomesRecombinant libraryKaryotypic studiesMetaphase spreadsChromosomal preparations
1985
Individual interphase chromosome domains revealed by in situ hybridization
Manuelidis L. Individual interphase chromosome domains revealed by in situ hybridization. Human Genetics 1985, 71: 288-293. PMID: 3908288, DOI: 10.1007/bf00388453.Peer-Reviewed Original ResearchConceptsInterphase chromosomesHuman chromosomesMouse-human cell hybridsCharacteristic folding patternSingle human chromosomeHuman interphase chromosomesSitu hybridizationHuman DNA probesInterphase chromosome domainsChromosome domainsIndividual chromosomesCell hybridsInterphase nucleiChromosomesDNA probesHybrid cellsFolding patternInterphase domainHybridizationOptical sectionsDomainNucleusWide fibersHybridization solutionReproducible position
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