1996
Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line.
Kearns W, Afione S, Fulmer S, Pang M, Erikson D, Egan M, Landrum M, Flotte T, Cutting G. Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line. Gene Therapy 1996, 3: 748-55. PMID: 8875221.Peer-Reviewed Original ResearchConceptsIB3-1 cellsWild-type AAVEpithelial cell lineMetaphase spreadsSite-specific fashionCFTR cDNACell linesLow molecular weight DNA fractionCF bronchial epithelial cell lineHuman chromosome 19Immortalized epithelial cell lineWeight DNA fractionMetaphase chromosome preparationsMetaphase chromosome spreadsBronchial epithelial cell lineDifferent chromosomesAAV-CFTR vectorCDNA integrationChromosome spreadsChromosome 19Genomic DNASite-specific mannerDNA fractionChromosome preparationsCFTR expression
1988
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries
Lichter P, Cremer T, Borden J, Manuelidis L, Ward D. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988, 80: 224-234. PMID: 3192212, DOI: 10.1007/bf01790090.Peer-Reviewed Original ResearchConceptsIndividual human chromosomesHuman chromosomesDNA insertsInterphase nucleiInterphase cellsTotal human genomic DNASitu suppression hybridizationPost-hybridization washesRecombinant DNA libraryHuman genomic DNAChromosomal domainsConfocal fluorescence microscopyChromosomal librarySuppression hybridizationDNA librarySingle-strand DNARepetitive sequencesCognate chromosomeChromosome 1Genomic DNAChromosomesRecombinant libraryKaryotypic studiesMetaphase spreadsChromosomal preparations
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