2008
Use of organotypic coculture to study keloid biology
Butler PD, Ly DP, Longaker MT, Yang GP. Use of organotypic coculture to study keloid biology. The American Journal Of Surgery 2008, 195: 144-148. PMID: 18070722, PMCID: PMC2245861, DOI: 10.1016/j.amjsurg.2007.10.003.Peer-Reviewed Original ResearchConceptsNormal human keratinocytesAnimal modelsKeloid formationOrganotypic skinKeloid biologyDermal contracturesDefinitive therapyFetal calf serumOrganotypic skin modelsCollagen depositionDermal thicknessMuscle actinOrganotypic skin culturesSkin culturesCollagen stainingPathologic scarsOrganotypic coculturesCollagen productionHuman keratinocytesSkinContractureCalf serumDermal layerDermal structuresEagle's medium
2004
17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells. International Journal Of Angiology 2004, 13: 67-70. DOI: 10.1007/s00547-004-1068-9.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells. International Journal Of Angiology 2004, 67-70. DOI: 10.1055/s-0031-1276450.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation
1995
Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity
Strazzabosco M, Poci C, Spirlì C, Zsembery A, Granato A, Massimino M, Crepaldi G. Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity. Hepatology 1995, 22: 588-597. PMID: 7635429, DOI: 10.1002/hep.1840220232.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAmmonium ChlorideAnimalsCarcinoma, HepatocellularCarrier ProteinsCattleCell DivisionDNAEpidermal Growth FactorFetal BloodFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationInsulin-Like Growth Factor IILiver NeoplasmsSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersTransforming Growth Factor alphaTumor Cells, CulturedConceptsInsulinlike growth factor IIEpidermal growth factorTGF-alphaGrowth factor IIHep G2 cellsGrowth factorFactor IIG2 cellsAdministration of EGFLiver growth factorCl/HCO3 exchangeExtrahepatic tumorsAcid/base homeostasisHepatic tumorsAcid loaderFetal calf serumAcid extrudersHepatoma cell linePresence of FCSAcid loadExchange activityMetabolic activationHCO3- exchangeProliferative stimulusAmilorideIntracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor-α, and insulinlike growth factor-II on Na+H+ exchange activity
Strazzabosco M, Poci C, Spirlì C, Zsembery A, Granato A, Massimino M, Crepaldi G. Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor-α, and insulinlike growth factor-II on Na+H+ exchange activity. Hepatology 1995, 22: 588-597. DOI: 10.1016/0270-9139(95)90584-7.Peer-Reviewed Original ResearchInsulinlike growth factor IIEpidermal growth factorGrowth factor IIHep G2 cellsGrowth factorFactor IIG2 cellsAdministration of EGFLiver growth factorCl/HCO3 exchangeExtrahepatic tumorsAcid/base homeostasisHepatic tumorsAcid loaderFetal calf serumAcid extrudersHepatoma cell linePresence of FCSAcid loadExchange activityMetabolic activationHCO3- exchangeProliferative stimulusTGFαAmiloride
1994
Insulin-like Growth Factor I Regulation of Swarm Rat Chondrosarcoma Chondrocytes in Culture
Seong S, Matsumura T, Lee F, Whelan M, Li X, Trippel S. Insulin-like Growth Factor I Regulation of Swarm Rat Chondrosarcoma Chondrocytes in Culture. Experimental Cell Research 1994, 211: 238-244. PMID: 8143769, DOI: 10.1006/excr.1994.1083.Peer-Reviewed Original ResearchConceptsContribution of IGFDose-dependent fashionSwarm rat chondrosarcoma chondrocytesI antibodiesRat chondrosarcoma chondrocytesNonspecific antibodyFetal calf serumInsulin-like growth factor actionCalf serumInsulin-like growth factor IInsulin-like growth factor I regulationInhibitory effectChondrosarcoma chondrocytesRole of IGFIGF-I actionPresence of IGFEfficacy of IGFGrowth factor IMinimal inhibitory effectSerum-free controlsPost-natal lifeHalf-maximal stimulationEpiphyseal growth plateGrowth factor actionSerum concentrations
1992
Extracellular matrix protein: gene expression and synthesis in cultured rat mesangial cells
ISHIMURA E, STERZEL R, MORII H, KASHGARIAN M. Extracellular matrix protein: gene expression and synthesis in cultured rat mesangial cells. 日本腎臓学会誌 1992, 34: 9-17. PMID: 1593802, DOI: 10.14842/jpnjnephrol1959.34.9.Peer-Reviewed Original ResearchConceptsGene expressionProtein synthesisExtracellular matrixNorthern blottingBasement membrane matrix componentsFetal calf serumCollagen IMesangial cellsMRNA expressionDNA amountSynthesis of ECMCellular confluenceECM productionCultured rat mesangial cellsMatrix componentsRat mesangial cellsExpressionLamininBlottingCellsCalf serumImmunocytochemical studyPrevious immunocytochemical studiesCIIIMRNA
1986
Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture.
Sterzel R, Lovett D, Foellmer H, Perfetto M, Biemesderfer D, Kashgarian M. Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture. American Journal Of Pathology 1986, 125: 130-40. PMID: 3535527, PMCID: PMC1888434.Peer-Reviewed Original ResearchConceptsMesangial cellsNecrotic cell debrisCollagen type IMesangial matrix productionCultured mesangial cellsGlomerular mesangial cellsIsolated rat glomeruliType ISpecific rabbit polyclonal antibodiesNodular fociFetal calf serumDiseased glomeruliMC proliferationRabbit polyclonal antibodyStellate cellsNormal glomeruliMatrix proteinsInterstitial collagen types ICell debrisImmunocytochemical studyNodular accumulationMetabolic stimuliIntracellular fibrilsRat glomeruliGlomeruli
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture
1975
Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis
Milstone L, Piatigorsky J. Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis. Developmental Biology 1975, 43: 91-100. PMID: 1149925, DOI: 10.1016/0012-1606(75)90133-5.Peer-Reviewed Original ResearchConceptsChick lens epitheliumΔ-crystallin synthesisProtein synthesisΔ-crystallinDifferential protein synthesisLens fiber cellsLens fiber differentiationLens epitheliumProtein degradationFiber cellsFiber differentiationBulk proteinTotal protein synthesisRate of synthesisIntracellular valineLabeling periodNumber of cellsProteinFetal calf serumCellsDifferential stimulationBiochemical changesCalf serumTotal proteinEpithelium
1971
21 COULTER COUNTER EVALUATION OF THE DEATH OF FIBROBLASTS MEDIATED BY SENSITIZED LYMPHOCYTES IN THE PRESENCE OF SPECIFIC ANTIGEN
Ruddle N. 21 COULTER COUNTER EVALUATION OF THE DEATH OF FIBROBLASTS MEDIATED BY SENSITIZED LYMPHOCYTES IN THE PRESENCE OF SPECIFIC ANTIGEN. 1971, 401-407. DOI: 10.1016/b978-0-12-107750-1.50034-0.Peer-Reviewed Original Research
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