2023
Squalene Biosynthesis in Engineered Escherichia Coli
Zhang W, Li Y, Xie L, Hu Y. Squalene Biosynthesis in Engineered Escherichia Coli. Pharmaceutical Fronts 2023, 05: e321-e329. DOI: 10.1055/s-0043-1776291.Peer-Reviewed Original ResearchMevalonate pathwaySqualene productionProduction of squaleneEngineered Escherichia coliSqualene synthasesChassis cellsMVA pathwayDimethylallyl diphosphateEscherichia coli strainsExpression strengthMetabolic fluxExtinct animalsXL1-BlueDifferent speciesSqualene biosynthesisEscherichia coliColi strainsGenesPathwayNatural triterpenoidIspH
2022
Draft Genome Sequences of Multidrug-Resistant Escherichia coli Isolated from River Water
Kassem I, Hassan J, Esseili M, Mann D, Osman M, Li S, Deng X. Draft Genome Sequences of Multidrug-Resistant Escherichia coli Isolated from River Water. Microbiology Resource Announcements 2022, 11: e00827-22. PMID: 36222705, PMCID: PMC9670951, DOI: 10.1128/mra.00827-22.Peer-Reviewed Original Research
2011
Expanding the Genetic Code of Escherichia coli with Phosphoserine
Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Söll D. Expanding the Genetic Code of Escherichia coli with Phosphoserine. Science 2011, 333: 1151-1154. PMID: 21868676, PMCID: PMC5547737, DOI: 10.1126/science.1207203.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acyl-tRNA SynthetasesAnticodonChloramphenicolChloramphenicol O-AcetyltransferaseCodon, TerminatorDrug Resistance, BacterialEscherichia coliGenetic CodeGenetic EngineeringHumansMAP Kinase Kinase 1Peptide Elongation Factor TuPhosphoserineProtein EngineeringProtein Modification, TranslationalRecombinant Fusion ProteinsRNA, BacterialRNA, Transfer, Amino Acid-SpecificRNA, Transfer, Amino AcylRNA, Transfer, CysTransfer RNA AminoacylationConceptsGenetic codeEF-TuMitogen-activated ERKQuality control functionTransfer RNAProtein engineeringEscherichia coli strainsKinase 1Phosphoamino acidsMolecular biologyEscherichia coliO-phosphoserineColi strainsGeneral utilityDisease researchCanonical positionPhosphoproteomePhosphoserineRNABiologyERKProteinSynthetaseColiResidues
1999
Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement
Ibba M, Losey H, Kawarabayasi Y, Kikuchi H, Bunjun S, Söll D. Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 418-423. PMID: 9892648, PMCID: PMC15151, DOI: 10.1073/pnas.96.2.418.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acyl-tRNA SynthetasesBase SequenceBorrelia burgdorferi GroupCloning, MolecularDiphosphatesEscherichia coliEvolution, MolecularGenes, ArchaealGenes, BacterialGenetic Complementation TestKineticsLysine-tRNA LigaseMethanococcusMolecular Sequence DataNucleic Acid ConformationPhylogenyRNA, Transfer, Amino AcylSequence Analysis, DNASubstrate SpecificityTranscription, GeneticConceptsClass II LysRSAminoacyl-tRNA synthetasesLysyl-tRNA synthetasesSubstrate recognitionMolecular basisBacterial class IClass II enzymesSequence-specific recognitionGene displacementTranslational apparatusTRNA recognitionEscherichia coli strainsLysRSLysRSsSame nucleotideSynthetasesDiscriminator baseUnrelated typesLysine activationCertain bacteriaII enzymesColi strainsTRNALysClass IEnzyme
1994
A Lactobacillus nifS-like gene suppresses an Escherichia coli transaminase B mutation
Leong-Morgenthaler P, Oliver S, Hottinger H, Söll D. A Lactobacillus nifS-like gene suppresses an Escherichia coli transaminase B mutation. Biochimie 1994, 76: 45-49. PMID: 8031904, DOI: 10.1016/0300-9084(94)90061-2.Peer-Reviewed Original ResearchConceptsNifS-like genesNifS-like proteinsNif gene productsNif proteinsNif genesGene productsNitrogen-fixing bacteriaGroup of enzymesRemarkable sequence homologyCysteine desulfuraseSequence conservationEfficient nitrogen fixationLeucine auxotrophyTransaminase BDiverse functionsSequence homologyNitrogen fixationEscherichia coli strainsProtein productsMetabolic pathwaysAzotobacter vinelandiiGenesB mutationsProteinDissimilar mutations
1992
Arabidopsis alternative oxidase sustains Escherichia coli respiration.
Kumar A, Söll D. Arabidopsis alternative oxidase sustains Escherichia coli respiration. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10842-10846. PMID: 1438286, PMCID: PMC50438, DOI: 10.1073/pnas.89.22.10842.Peer-Reviewed Original ResearchConceptsAlternative oxidaseArabidopsis thaliana cDNA libraryGlutamyl-tRNA reductaseCyanide-insensitive respiratory pathwayAlternative oxidase activityAmino acid sequenceArabidopsis proteinsHemA geneMolecular biological investigationsCDNA libraryFirst enzymeAcid sequenceSauromatum guttatumEscherichia coli strainsSingle polypeptideRespiratory pathwayAerobic respirationRedox enzymesE. coliColi strainsPorphyrin biosynthesisGenesEnzymeProteinBiological investigations
1979
Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. VI. Mutants with increased levels of glutaminyl-transfer ribonucleic acid synthetase and of glutamine transfer ribonucleic acid
Cheung A, Morgan S, Low K, Söll D. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. VI. Mutants with increased levels of glutaminyl-transfer ribonucleic acid synthetase and of glutamine transfer ribonucleic acid. Journal Of Bacteriology 1979, 139: 176-184. PMID: 378954, PMCID: PMC216843, DOI: 10.1128/jb.139.1.176-184.1979.Peer-Reviewed Original ResearchConceptsTransfer ribonucleic acidAminoacyl-transfer ribonucleic acid synthetasesE. coli mapGlutaminyl-tRNA synthetaseRibonucleic acidTRNA-GlnStructural geneGenetic lociEscherichia coli strainsSpontaneous revertantsEscherichia coliParental strainThermolabile enzymeInteresting strainsColi strainsSynthetaseLociRevertantsEnzymeGlnMin 24GlnRStrainsTRNAMutantsRegulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase
Baer M, Low K, Söll D. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase. Journal Of Bacteriology 1979, 139: 165-175. PMID: 378953, PMCID: PMC216842, DOI: 10.1128/jb.139.1.165-175.1979.Peer-Reviewed Original ResearchConceptsValyl-tRNA synthetaseAminoacyl-transfer ribonucleic acid synthetasesTransfer ribonucleic acidStructural geneProtein degradationEnzyme overproductionGenetic lociEscherichia coli strainsSpontaneous revertantsEscherichia coliThermolabile enzymeEnzyme synthesisSynthetaseNew enzyme synthesisColi strainsRibonucleic acidRevertantsTurnover rateSynthetasesMutantsBiosynthesisGenesLociColiEnzyme
1977
Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli II. Isolation of regulatory mutants affecting leucyl-tRNA synthetase levels
LaRossa R, Vögeli G, Low K, Söll D. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli II. Isolation of regulatory mutants affecting leucyl-tRNA synthetase levels. Journal Of Molecular Biology 1977, 117: 1033-1048. PMID: 342703, DOI: 10.1016/s0022-2836(77)80011-9.Peer-Reviewed Original ResearchConceptsLeucyl-tRNA synthetaseThermolabile leucyl-tRNA synthetaseRegulation of biosynthesisDistinct genetic lociAminoacyl-tRNA synthetasesEscherichia coli IIOperator-promoter regionStructural geneRegulatory mutantsGenetic lociEscherichia coli strainsSuch revertantsSpontaneous revertantsThermolabile enzymeLociSynthetaseColi strainsRevertantsSynthetase levelsLeuTRNAMutantsSynthetasesLeuXBiosynthesisRegulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli I. Isolation and characterization of a mutant with elevated levels of tRNAGln1
Morgan S, Körner A, Low K, Söll D. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli I. Isolation and characterization of a mutant with elevated levels of tRNAGln1. Journal Of Molecular Biology 1977, 117: 1013-1031. PMID: 24122, DOI: 10.1016/s0022-2836(77)80010-7.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseAminoacyl-tRNA synthetasesRegulation of biosynthesisEscherichia coli strainsGlutamine synthetaseSpontaneous revertantsSynthetasesColi strainsMutantsSynthetaseElevated levelsRevertantsI. IsolationTRNABiosynthesisDuplicationSpeciesMutationsGlnTRegulationOverproductionIsolation
1971
Purification of an Escherichia coli Leucine Suppressor Transfer Ribonucleic Acid and Its Aminoacylation by the Homologous Leucyl-Transfer Ribonucleic Acid Synthetase
Hayashi H, Söll D. Purification of an Escherichia coli Leucine Suppressor Transfer Ribonucleic Acid and Its Aminoacylation by the Homologous Leucyl-Transfer Ribonucleic Acid Synthetase. Journal Of Biological Chemistry 1971, 246: 4951-4954. PMID: 4941862, DOI: 10.1016/s0021-9258(18)61955-6.Peer-Reviewed Original ResearchMeSH KeywordsAcylationBenzoatesBiological AssayCarbon IsotopesChromatography, DEAE-CelluloseColiphagesEscherichia coliGenetics, MicrobialKineticsLeucineLigasesMutationNicotianaPeptide BiosynthesisPlant Growth RegulatorsPlants, ToxicPolynucleotidesRNA, TransferSuppression, GeneticTemplates, GeneticValine
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