2001
Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8
Colmenares M, Tiemeyer M, Kima P, McMahon-Pratt D. Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8. Infection And Immunity 2001, 69: 6776-6784. PMID: 11598050, PMCID: PMC100055, DOI: 10.1128/iai.69.11.6776-6784.2001.Peer-Reviewed Original ResearchConceptsAntigen PFurther vaccine studiesSignificant T cell activationT cell activationWestern blot analysisProtective immunityVaccine studiesApolipoprotein EMurine modelCutaneous leishmaniasisSignificant protectionComparable protectionImmunodominant componentAntigenic glycolipidsImmunity studiesAntigenCysteine proteinasesGlycolipid componentBiochemical analysisBiological characterizationSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisInfectionLeishmaniasis
1998
Use of liquid chromatography‐electrospray ionization‐tandem mass spectrometry (LC‐ESI‐MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis
Stone K, Deangelis R, LoPresti M, Jones J, Papov V, Williams K. Use of liquid chromatography‐electrospray ionization‐tandem mass spectrometry (LC‐ESI‐MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Electrophoresis 1998, 19: 1046-1052. PMID: 9638951, DOI: 10.1002/elps.1150190620.Peer-Reviewed Original ResearchConceptsSodium dodecyl sulfate-polyacrylamide gel electrophoresisQuadrupole ion trap mass spectrometerIon trap mass spectrometerDodecyl sulfate-polyacrylamide gel electrophoresisLow pmol levelSulfate-polyacrylamide gel electrophoresisIonization tandem mass spectrometryTrap mass spectrometerLiquid chromatography-electrospray ionization-tandem mass spectrometryLiquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysisMS/MS approachProtein identificationIonization tandem mass spectrometry analysisFmol levelFacile approachMass spectrometry analysisMass spectrometerEng et alMass spectrometryPmol levelLC-MS/MS approachTryptic digestMS approachSpectrometry analysisGel electrophoresis
1996
Purification and characterization of a recombinant hepatitis E protein vaccine candidate by liquid chromatography-mass spectrometry
McAtee C, Zhang Y, Yarbough P, Fuerst T, Stone K, Samander S, Williams K. Purification and characterization of a recombinant hepatitis E protein vaccine candidate by liquid chromatography-mass spectrometry. Journal Of Chromatography B 1996, 685: 91-104. PMID: 8930757, DOI: 10.1016/0378-4347(96)00143-0.Peer-Reviewed Original ResearchConceptsMass spectrometryCarboxyl terminusReversed phase liquid chromatographyAmino terminusLaser desorption mass spectrometryDesorption mass spectrometryMolecular massLiquid chromatography-mass spectrometryCarboxyl-terminal sequencingChromatography-mass spectrometryBaculovirus expression vectorSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisLC-MSLiquid chromatographyExpression vectorTerminal sequencingSequence analysisPolyacrylamide gel electrophoresisSpectrometryIntact proteinInternal sequencesDoublet proteinsTerminus
1993
Purification and characterization of calmodulin-dependent protein kinase III from rabbit reticulocytes and rat pancreas
Mitsui K, Brady M, Palfrey H, Nairn A. Purification and characterization of calmodulin-dependent protein kinase III from rabbit reticulocytes and rat pancreas. Journal Of Biological Chemistry 1993, 268: 13422-13433. PMID: 8514778, DOI: 10.1016/s0021-9258(19)38667-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Calmodulin-Dependent Protein KinasesCalmodulinCattleChromatography, GelElectrophoresis, Polyacrylamide GelElongation Factor 2 KinaseHeat-Shock ProteinsMolecular Sequence DataPancreasPeptide Elongation Factor 2Peptide Elongation FactorsPeptide MappingPhosphoproteinsPhosphorylationProtein KinasesRabbitsRatsReticulocytesSubstrate SpecificityConceptsEukaryotic elongation factor 2CaM kinase IIICalmodulin-dependent protein kinase IIIProtein kinase IIIKinase IIIProtein kinaseRabbit reticulocytesCAMP-dependent protein kinaseYeast EF-2Heat shock protein Hsp90Novel protein kinaseElongation factor 2Amino acid sequencingPhosphopeptide mappingSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisProtein Hsp90Catalytic subunitSulfate-polyacrylamide gel electrophoresisSeryl residuesMajor polypeptidesSubstrate ATPHsp90Factor 2Gel electrophoresisIdentification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line
Nillni E, Sevarino K, Jackson I. Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line. Endocrinology 1993, 132: 1260-1270. DOI: 10.1210/en.132.3.1260.Peer-Reviewed Original ResearchSodium dodecyl sulfate-polyacrylamide gel electrophoresisCryptic peptidesDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisAtT20 cell lineCell linesPulse-chase studiesN-terminal fragmentGel filtration chromatographyCarboxy-terminalCell extractsGel electrophoresisPulse-chaseTRH progenitor sequenceProgenitor sequencePosttranslational processingWestern blot analysisAmino acidsBiosynthetic precursorFiltration chromatographyBlot analysisTumor cell linesGln-His-Pro-GlySequenceProTRH-derived peptides
1992
Fecal protein markers of colorectal cancer.
Dubrow R, Yannielli L. Fecal protein markers of colorectal cancer. The American Journal Of Gastroenterology 1992, 87: 854-8. PMID: 1615938.Peer-Reviewed Original ResearchConceptsColorectal cancerControl stoolsStool supernatantsEarly detectionProtein immunoblottingSodium dodecyl sulfate-polyacrylamide gel electrophoresisStoolCancerHuman albuminFecal proteinBlue stainingProtein markersImmunoblottingBlood proteinsSupernatantDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisPatientsCoomassie blue stainingFurther workProteinHuman hemoglobinConsistent differencesHemoglobin
1991
Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties.
Pande S, Jahn D, Söll D. Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties. Journal Of Biological Chemistry 1991, 266: 22826-22831. PMID: 1660461, DOI: 10.1016/s0021-9258(18)54428-8.Peer-Reviewed Original ResearchConceptsAdditional nucleotidesHistidine tRNA genesPolymin P precipitationTRNA genesSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisTRNA speciesSulfate-polyacrylamide gel electrophoresisRate zonal sedimentationHomodimeric structureGuanylyltransferaseRelative molecular weightTRNAATP-agaroseGel filtrationAbolishes activityHistidine tRNANative enzymeGuanosine residuesAcceptor RNAEnzymatic activityUnfractionated tRNAGuanosine substrateZonal sedimentationGel electrophoresisGlycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant ProteinsConceptsBioactive speciesTrifluoromethanesulfonic acidSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisThe Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase
Ilag L, Jahn D, Eggertsson G, Söll D. The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase. Journal Of Bacteriology 1991, 173: 3408-3413. PMID: 2045363, PMCID: PMC207952, DOI: 10.1128/jb.173.11.3408-3413.1991.Peer-Reviewed Original ResearchMeSH KeywordsAminolevulinic AcidCentrifugation, Density GradientChromatography, High Pressure LiquidCloning, MolecularDose-Response Relationship, DrugElectrophoresis, Polyacrylamide GelEscherichia coliIntramolecular TransferasesIsomerasesMolecular WeightPyridoxal PhosphatePyridoxamineTransformation, GeneticConceptsGlu-tRNA reductaseTRNA-dependent transformationApparent native molecular massMolecular massGlutamyl-tRNA synthetaseNative molecular massAminoglycoside antibiotic kanamycinHemL geneWild-type DNAAuxotrophic phenotypeC5 pathwaySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisMap positionGSA aminotransferasePhysical mappingSulfate-polyacrylamide gel electrophoresisRate zonal sedimentationGene productsThird enzymeGlycerol gradientsApparent homogeneityAntibiotic kanamycinEscherichia coliPure proteinPurification and functional characterization of glutamate-1-semialdehyde aminotransferase from Chlamydomonas reinhardtii.
Jahn D, Chen M, Söll D. Purification and functional characterization of glutamate-1-semialdehyde aminotransferase from Chlamydomonas reinhardtii. Journal Of Biological Chemistry 1991, 266: 161-167. PMID: 1985889, DOI: 10.1016/s0021-9258(18)52416-9.Peer-Reviewed Original ResearchMeSH KeywordsAminooxyacetic AcidCell MembraneChlamydomonasChromatography, DEAE-CelluloseChromatography, GelChromatography, High Pressure LiquidChromatography, Ion ExchangeCyclohexanecarboxylic AcidsElectrophoresis, Polyacrylamide GelIntramolecular TransferasesIsomerasesKineticsMolecular WeightPyridoxal PhosphateConceptsGlutamate-1-semialdehyde aminotransferaseGlutamyl-tRNA synthetaseC5 pathwayChlamydomonas reinhardtiiGreen alga Chlamydomonas reinhardtiiGlu-tRNA reductaseTRNA-dependent transformationChloroplasts of plantsGlutamyl-tRNA reductaseAlga Chlamydomonas reinhardtiiDelta-aminolevulinic acidApparent molecular massWhole cell extractsChlorophyll biosynthesisSodium dodecyl sulfate-polyacrylamide gel electrophoresisC. reinhardtiiDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisRate zonal sedimentationFunctional characterizationThird enzymeGlycerol gradientsCell extractsReinhardtiiMembrane fraction
1990
Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis
Hotez P, Haggerty J, Hawdon J, Milstone L, Gamble H, Schad G, Richards F. Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infection And Immunity 1990, 58: 3883-3892. PMID: 2254016, PMCID: PMC313750, DOI: 10.1128/iai.58.12.3883-3892.1990.Peer-Reviewed Original ResearchConceptsProtease activitySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisProtein substratesSulfate-polyacrylamide gel electrophoresisMr polypeptideNematode Strongyloides stercoralisBovine elastinPossible functionsBiochemical similaritiesAlkaline pH optimumLarvaeConnective tissue macromoleculesHuman hookworms Ancylostoma duodenaleInvasion propertiesGel electrophoresisEnzyme activityAncylostoma speciesBiochemical differencesA. caninum larvaeMinor bandsProtease compositionSmall peptidesHuman fibronectinMajor bandRegulated phosphorylation of secretory granule membrane proteins of the rat parotid gland
Marino CR, Castle JD, Gorelick FS. Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland. American Journal Of Physiology 1990, 259: g70-g77. PMID: 1695488, DOI: 10.1152/ajpgi.1990.259.1.g70.Peer-Reviewed Original ResearchConceptsSecretory granule membrane proteinsIntegral membrane proteinsMembrane proteinsGranule membrane proteinProtein phosphorylation eventsGranule membrane fractionExocrine secretory granulesPhosphorylation eventsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisRat parotid glandRegulated phosphorylationSulfate-polyacrylamide gel electrophoresisSitu phosphorylationIntact cellsMembrane fractionTime-dependent mannerPhosphorylationProteinSecretory granule fractionParotid lobulesGel electrophoresisProtein antiserumSecretory granulesPhosphoprotein
1988
Purification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000
Weeks G, Picciotto M, Nairn A, Walaas S, Greengard P. Purification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000. Synapse 1988, 2: 89-96. PMID: 2844000, DOI: 10.1002/syn.890020112.Peer-Reviewed Original ResearchConceptsCAMP-dependent protein kinaseMembrane proteinsProtein kinaseN-lauryl sarcosineIntegral membrane proteinsMajor tryptic phosphopeptidesPhosphoamino acid analysisTotal membrane proteinSodium dodecyl sulfate-polyacrylamide gel electrophoresisMembrane phosphoproteinDodecyl sulfate-polyacrylamide gel electrophoresisTryptic phosphopeptidesSulfate-polyacrylamide gel electrophoresisPossible functional roleProminent proteinsAlpha-methyl mannosideParticulate fractionMammalian cerebellumFunctional roleProteinPeptide mappingConcanavalin A-agaroseGel electrophoresisAcid analysisA-agarose
1985
Characterization of the tissue form of type V collagen from chick bone.
Broek D, Madri J, Eikenberry E, Brodsky B. Characterization of the tissue form of type V collagen from chick bone. Journal Of Biological Chemistry 1985, 260: 555-562. PMID: 3965462, DOI: 10.1016/s0021-9258(18)89768-x.Peer-Reviewed Original ResearchConceptsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisGlobular domainType V collagenCyanogen bromide peptide mapsTriple-helical domainGel electrophoresisIsolated alpha chainHelical domainTriple helixForm of alphaBanding patternsChick bonePeptide mapsProtein bandsBacterial collagenase digestionTerminal peptidesRotary shadowingHigh molecular weight formAlpha chainTissue formMolecular weight formsSlower mobilityHelix
1981
The carboxyl-terminal domain of human erythrocyte band 3. Description, isolation, and location in the bilayer.
Markowitz S, Marchesi V. The carboxyl-terminal domain of human erythrocyte band 3. Description, isolation, and location in the bilayer. Journal Of Biological Chemistry 1981, 256: 6463-6468. PMID: 7240219, DOI: 10.1016/s0021-9258(19)69187-8.Peer-Reviewed Original ResearchConceptsSodium dodecyl sulfate bindingBand 3 moleculesCarboxyl-terminal fragmentBand 3NH2-terminal cytoplasmic domainMembrane-spanning domainsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisBand 3 fragmentSulfate-polyacrylamide gel electrophoresisCarboxyl-terminal domainAnion channel proteinCarboxyl-terminal regionPolyacrylamide gel electrophoresisGel electrophoresisSulfate bindingHomogeneous polypeptideCytoplasmic domainIdentical peptide mapsMoleculesMajor transmembraneHuman erythrocyte band 3Channel proteinsBroad bandErythrocyte band 3
1978
Phenylalanine hydroxylase in melanoma cells
Breakefield X, Castiglione C, Halaban R, Pawelek J, Shiman R. Phenylalanine hydroxylase in melanoma cells. Journal Of Cellular Physiology 1978, 94: 307-314. PMID: 23386, DOI: 10.1002/jcp.1040940308.Peer-Reviewed Original ResearchConceptsPhenylalanine hydroxylase activityHydroxylase activityMelanoma cellsMelanoma cell extractsS91 melanoma cellsCloudman S91 melanoma cellsP-chlorophenylalanineTyrosine hydroxylasePhenylalanine hydroxylaseTryptophan hydroxylaseHydroxylaseConversion of phenylalanineSensitive assayTyrosinase activityCellsLiver phenylalanine hydroxylaseCell extractsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisLatter activityActivity
1976
Three structural polypeptides coded for by minite virus of mice, a parvovirus
Tattersall P, Cawte P, Shatkin A, Ward D. Three structural polypeptides coded for by minite virus of mice, a parvovirus. Journal Of Virology 1976, 20: 273-289. PMID: 988192, PMCID: PMC354988, DOI: 10.1128/jvi.20.1.273-289.1976.Peer-Reviewed Original ResearchConceptsLate maturation stepPulse-chase experimentsNuclei of cellsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisDNA genomeSulfate-polyacrylamide gel electrophoresisThird polypeptideMaturation stepsVirion massProtein componentsHost cellsEmpty virionsStructural polypeptidesPolypeptideMinute virusGel electrophoresisCsCl gradientsProtein massUninfected cellsGrowth conditionsSequential harvestingEmpty particlesVirionsProteinStudies of muscle proteins in embryonic myocardial cells of cardiac lethal mutant mexican axolotls (Ambystoma mexicanum) by use of heavy meromyosin binding and sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Lemanski L, Mooseker M, Peachey L, Iyengar M. Studies of muscle proteins in embryonic myocardial cells of cardiac lethal mutant mexican axolotls (Ambystoma mexicanum) by use of heavy meromyosin binding and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Journal Of Cell Biology 1976, 68: 375-388. PMID: 1107335, PMCID: PMC2109630, DOI: 10.1083/jcb.68.2.375.Peer-Reviewed Original ResearchConceptsMutant heartsRecessive mutant gene cMutant siblingsGene CHeart muscle differentiationMutant heart cellsHeavy meromyosin treatmentDalton bandMuscle proteinsGel electrophoresisSingle gene mutationsMutant embryonic heartsDifferent muscle proteinsEmbryonic myocardial cellsEarly developmental stagesSkeletal muscleSodium dodecyl sulfate gel electrophoresisDodecyl sulfate gel electrophoresisEmbryonic heart functionSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisHeavy meromyosin bindingMuscle differentiationMexican axolotl
1975
Isolation and characterization of a novel vitamin B12-binding protein associated with hepatocellular carcinoma.
Burger R, Waxman S, Gilbert H, Mehlman C, Allen R. Isolation and characterization of a novel vitamin B12-binding protein associated with hepatocellular carcinoma. Journal Of Clinical Investigation 1975, 56: 1262-1270. PMID: 171283, PMCID: PMC301989, DOI: 10.1172/jci108202.Peer-Reviewed Original ResearchConceptsVitamin B12-binding proteinsHepatocellular carcinomaR-type proteinNormal liver tissueCertain patientsAdolescent patientsPleural fluidHuman milkSame patientPatientsSialic acid contentTumor tissueImmunologic propertiesLiver tissueSurvival studiesMore sialic acidCarcinomaPlasma survivalHigh levelsSialic acidImmunoprecipitation assaysTissueSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisApparent molecular weight
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply