2024
DNA-Based Molecular Clamp for Probing Protein Interactions and Structure under Force
Chung M, Zhou K, Powell J, Lin C, Schwartz M. DNA-Based Molecular Clamp for Probing Protein Interactions and Structure under Force. ACS Nano 2024, 18: 27590-27596. PMID: 39344156, PMCID: PMC11518680, DOI: 10.1021/acsnano.4c08663.Peer-Reviewed Original ResearchConceptsTalin rod domainNegative-stain electron microscopyDouble-stranded DNADNA clampProtein functionRod domainCryptic sitesProtein interactionsMolecular clampCellular mechanotransductionStudy proteinsBiochemical studiesCell biologyAdult physiologyProtein conformationTalinProteinBiochemical scaleMultiple diseasesDNAARPC5LVinculinStructural analysisEmbryogenesisDNA-based devices
2022
Actuating tension-loaded DNA clamps drives membrane tubulation
Liu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.Peer-Reviewed Original ResearchConceptsDNA clampMembrane tubulationMembrane dynamicsMembrane-remodeling eventsVesicle tubulationConformational changesSpatiotemporal controlDNA signalsCell membraneDNA nanostructuresTubulationMembrane deformationClosed stateOpen stateSelf-assembled DNA nanostructuresOrganismsProteinMembrane tubeArtificial systemsTube widthMembraneDynamics
2002
Monomeric Yeast PCNA Mutants Are Defective in Interacting with and Stimulating the ATPase Activity of RFC †
Ionescu CN, Shea KA, Mehra R, Prundeanu L, McAlear MA. Monomeric Yeast PCNA Mutants Are Defective in Interacting with and Stimulating the ATPase Activity of RFC †. Biochemistry 2002, 41: 12975-12985. PMID: 12390024, DOI: 10.1021/bi026029s.Peer-Reviewed Original ResearchConceptsReplication factor CMutant PCNAsDNA clampDNA damaging agent methylmethane sulfonateDNA polymerase accessory proteinMutant PCNA proteinsAgent methylmethane sulfonatePolymerase accessory proteinsProtein-protein interactionsWild-type subunitsSingle amino acid substitutionDNA repair pathwaysATPase activityE. coliAmino acid substitutionsYeast PCNAPCNA mutantsClamp loaderPCNA functionPCNA trimerBeta clampAccessory proteinsRepair pathwaysMethylmethane sulfonateAdjacent subunits
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