2025
Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders
Odierna G, Stednitz S, Pruitt A, Arnold J, Hoffman E, Scott E. Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders. Journal Of Neuroscience Methods 2025, 418: 110426. PMID: 40086601, DOI: 10.1016/j.jneumeth.2025.110426.Peer-Reviewed Original ResearchBrain cell culturesCell culturesCell typesNeuronal culture protocolCultured primary neuronsEmbryonic zebrafishPrimary brain cell culturesZebrafish mutantsCellular consequencesStructural hallmarksMature synaptic connectionsPrimary neuronsCell survivalMammalian tissuesTranscriptional signatureCell linesEmbryonic tissuesZebrafish neuronsZebrafishNeuron purityNeuronal culturesNetwork of neuronsMixed cell typeDays in vitroModel system
2023
High-throughput functional analysis of autism genes in zebrafish identifies convergence in dopaminergic and neuroimmune pathways
Mendes H, Neelakantan U, Liu Y, Fitzpatrick S, Chen T, Wu W, Pruitt A, Jin D, Jamadagni P, Carlson M, Lacadie C, Enriquez K, Li N, Zhao D, Ijaz S, Sakai C, Szi C, Rooney B, Ghosh M, Nwabudike I, Gorodezky A, Chowdhury S, Zaheer M, McLaughlin S, Fernandez J, Wu J, Eilbott J, Vander Wyk B, Rihel J, Papademetris X, Wang Z, Hoffman E. High-throughput functional analysis of autism genes in zebrafish identifies convergence in dopaminergic and neuroimmune pathways. Cell Reports 2023, 42: 112243. PMID: 36933215, PMCID: PMC10277173, DOI: 10.1016/j.celrep.2023.112243.Peer-Reviewed Original ResearchConceptsGene lossFunctional analysisHigh-throughput functional analysisZebrafish mutantsGene discoverySelect mutantsASD genesAutism genesKey pathwaysASD biologyBrain size differencesMutantsGenesSize differencesPathwayGlobal increaseRelevant mechanismsBiologyCentral challengeNeuroimmune dysfunctionRegionFunctionDiscoveryAutism spectrum disorder
2022
Zebrafish mutants in vegfab can affect endothelial cell proliferation without altering ERK phosphorylation and are phenocopied by loss of PI3K signaling
Lange M, Ohnesorge N, Hoffmann D, Rocha SF, Benedito R, Siekmann AF. Zebrafish mutants in vegfab can affect endothelial cell proliferation without altering ERK phosphorylation and are phenocopied by loss of PI3K signaling. Developmental Biology 2022, 486: 26-43. PMID: 35337795, PMCID: PMC11238767, DOI: 10.1016/j.ydbio.2022.03.006.Peer-Reviewed Original ResearchConceptsEndothelial cell migrationLong isoformPI3KCell migrationEC proliferationZebrafish trunkZebrafish mutantsMutant phenotypeCellular functionsIntersegmental blood vesselsMRNA splicingPI3-kinaseMAPK activationVegfabA PathwayERK phosphorylationExtracellular matrixEndothelial cell proliferationVEGFA isoformsIsoformsCell proliferationDistinct setsProliferationBlood vessel networkMutations
2021
TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice
Moyon S, Frawley R, Marechal D, Huang D, Marshall-Phelps KLH, Kegel L, Bøstrand SMK, Sadowski B, Jiang YH, Lyons DA, Möbius W, Casaccia P. TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice. Nature Communications 2021, 12: 3359. PMID: 34099715, PMCID: PMC8185117, DOI: 10.1038/s41467-021-23735-3.Peer-Reviewed Original ResearchConceptsDNA hydroxymethylationSolute carrier gene familyNeuro-glial communicationZebrafish mutantsGene familyTranscriptomic analysisMyelin interfaceTen-ElevenAdult central nervous systemCentral nervous systemTET1Overexpressing cellsAdult remyelinationExpression levelsMutantsHydroxymethylationGenesNervous systemRepairMyelin repairTransportersKnockoutMiceRegulationAged mice
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