2023
Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species
Asin-Garcia E, Garcia-Morales L, Bartholet T, Liang Z, Isaacs F, dos Santos V. Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species. Nucleic Acids Research 2023, 51: 12522-12536. PMID: 37941137, PMCID: PMC10711431, DOI: 10.1093/nar/gkad1024.Peer-Reviewed Original ResearchConceptsPseudomonas genusMetabolic engineering effortsSynthetic biology applicationsGenetic engineering toolsGenome editing capabilitiesRelated speciesStress resistanceValuable hostBiology applicationsActive variantsEnvironmental biotechnologyDifferent PseudomonasRecombineeringPseudomonas speciesPseudomonas putidaPseudomonas fluorescensSpeciesGenusNGS analysisCharacteristics of toleranceRemarkable abilityProteinEngineering effortsPseudomonasValuable compounds
2022
Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome
Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Cell 2022, 185: 1487-1505.e14. PMID: 35366417, PMCID: PMC10619838, DOI: 10.1016/j.cell.2022.03.008.Peer-Reviewed Original ResearchConceptsSynthetic genetic elementsGenetic elementsBiosynthetic gene clusterCross-species expressionCross-species interactionsDiverse organismsGene clusterBiosynthetic machineryHeterologous expressionRegulatory regionsTRNA synthetasesBiosynthetic pathwayNative contextTranslational activityBiosynthetic capacityHuman microbiomeMetabolic capacityPositive bacteriaSmall moleculesExpressionPathwayValuable compoundsLactobacillus inersEukaryotesSynthetasesSynthetic Biology in the Candida (CTG) Clade
Kasir D, Besseau S, Clastre M, Oudin A, Hamze M, Courdavault V, Osman M, Papon N. Synthetic Biology in the Candida (CTG) Clade. 2022, 337-375. DOI: 10.1007/978-3-030-89680-5_12.Peer-Reviewed Original Research
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply