2017
The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei
Chikne V, Gupta SK, Doniger T, K. SR, Cohen-Chalamish S, Ben-Asher HW, Kolet L, Yahia NH, Unger R, Ullu E, Kolev NG, Tschudi C, Michaeli S. The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei. Journal Of Molecular Biology 2017, 429: 3301-3318. PMID: 28456523, DOI: 10.1016/j.jmb.2017.04.015.Peer-Reviewed Original ResearchConceptsNon-coding RNAsPoly (ADP-ribose) polymeraseUnique RNA processing mechanismsCis-spliced intronsRNA processing mechanismsSmall nucleolar RNAsLong non-coding RNAsPolyadenylation of mRNAParasite Trypanosoma bruceiSnoRNA biogenesisTrans splicingPrecursor snoRNASnoRNA processingNucleolar RNAsPAP1Trypanosoma bruceiPolyadenylationMajor substrateSnoRNAsTrypanosoma cruziPAP2RNAPolymerase Pap1TrypanosomesCausative agent
2008
A Neoplastic Gene Fusion Mimics Trans-Splicing of RNAs in Normal Human Cells
Li H, Wang J, Mor G, Sklar J. A Neoplastic Gene Fusion Mimics Trans-Splicing of RNAs in Normal Human Cells. Science 2008, 321: 1357-1361. PMID: 18772439, DOI: 10.1126/science.1156725.Peer-Reviewed Original ResearchMeSH KeywordsCell HypoxiaCell LineCell Line, TumorChromosome AberrationsChromosomes, Human, Pair 17Chromosomes, Human, Pair 7Co-Repressor ProteinsDeferoxamineDNA-Binding ProteinsEndometrial NeoplasmsEndometriumExonsFemaleGene FusionHumansMenstrual CycleMutant Chimeric ProteinsNeoplasm ProteinsProgesteroneProtein BiosynthesisRNA PrecursorsRNA, MessengerStromal CellsTrans-SplicingTranscription FactorsTranslocation, GeneticConceptsGene fusionsChimeric RNAsPrecursor messenger RNAChimeric gene productsResultant chimeric transcriptsTrans splicingNormal human cellsAnti-apoptotic activityChromosome 7p15Gene productsChromosomal rearrangementsChromosome 17q11JAZF1 geneHuman cellsTumor-specific productsRNANormal endometrial stromal cellsChimeric transcriptsMessenger RNAExonsProteinHuman tumorsStromal cellsEndometrial stromal cellsCells
1998
Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei
López-Estraño C, Tschudi C, Ullu E. Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei. Molecular And Cellular Biology 1998, 18: 4620-4628. PMID: 9671472, PMCID: PMC109048, DOI: 10.1128/mcb.18.8.4620.Peer-Reviewed Original ResearchTrypanosome Capping Enzymes Display a Novel Two-Domain Structure
Silva E, Ullu E, Kobayashi R, Tschudi C. Trypanosome Capping Enzymes Display a Novel Two-Domain Structure. Molecular And Cellular Biology 1998, 18: 4612-4619. PMID: 9671471, PMCID: PMC109047, DOI: 10.1128/mcb.18.8.4612.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCloning, MolecularConserved SequenceCrithidia fasciculataDNA, ProtozoanGuanosine MonophosphateHumansMiceMolecular Sequence DataRecombinant Fusion ProteinsRNA NucleotidyltransferasesSequence Homology, Amino AcidTrypanosoma brucei bruceiConceptsSpliceosomal small nuclear RNAsM7G capCapping enzymeTwo-domain structureG capYeast capping enzymesSmall nuclear RNAPhosphate-binding loopAmino-terminal halfCarboxy-terminal halfGTP-binding proteinsDiphosphate endTrans splicingSignature motifTranscript elongationM7G residueSL sequenceTrypanosomatid protozoaEukaryotic mRNAsLeader RNAGene codesRNA modificationsNuclear RNAOverall identityTrypanosoma brucei
1996
Trans -splicing in trypanosotnatid protozoa
Ullu E, Tschudi C, Gunzl A. Trans -splicing in trypanosotnatid protozoa. 1996, 115-133. DOI: 10.1093/oso/9780199636020.003.0007.Peer-Reviewed Original ResearchTrans splicingSplice siteT. brucei cellsPolycistronic pre-mRNARNA processing reactionsEnd of transcriptsDifferent variant surface glycoproteinsSeparate RNA moleculesVariant surface glycoproteinSL RNAList of organismsSmall RNAsBrucei cellsSL sequenceTrypanosomatid protozoaMature mRNALeader RNAMature speciesPre-mRNATrypanosoma bruceiRNA moleculesProcessing reactionsRNASurface glycoproteinFirst evidence
1993
Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.
Ullu E, Matthews K, Tschudi C. Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Molecular And Cellular Biology 1993, 13: 720-725. PMID: 8417363, PMCID: PMC358950, DOI: 10.1128/mcb.13.1.720.Peer-Reviewed Original ResearchConceptsRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingAlpha-tubulinPolyadenylationMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesisGenesTranscriptsRNAOrganismsTemporal Order of RNA-Processing Reactions in Trypanosomes: Rapid trans Splicing Precedes Polyadenylation of Newly Synthesized Tubulin Transcripts
Ullu E, Matthews K, Tschudi C. Temporal Order of RNA-Processing Reactions in Trypanosomes: Rapid trans Splicing Precedes Polyadenylation of Newly Synthesized Tubulin Transcripts. Molecular And Cellular Biology 1993, 13: 720-725. DOI: 10.1128/mcb.13.1.720-725.1993.Peer-Reviewed Original ResearchRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsRNA processing reactionsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingPolyadenylationΒ-tubulinΑ-tubulinMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesis
1991
Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Ullu E, Tschudi C. Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 10074-10078. PMID: 1719544, PMCID: PMC52870, DOI: 10.1073/pnas.88.22.10074.Peer-Reviewed Original Research
1990
Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells
Tschudi C, Ullu E. Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells. Cell 1990, 61: 459-466. PMID: 1692263, DOI: 10.1016/0092-8674(90)90527-l.Peer-Reviewed Original ResearchConceptsTrans splicingU snRNAsPre-mRNAU6 small nuclear RNAU4/U6 snRNPsAlpha-tubulin transcriptsSmall nuclear RNAHigh molecular weight RNAMolecular weight RNASite-directed cleavageSL sequenceLeader RNATrypanosome cellsU6 snRNPsNuclear RNAU6 snRNATrypanosoma bruceiSplicing processSplicingWeight RNASnRNAEarly stepsRNAase HRNAIntrons
1988
Trans splicing involves a novel form of small nuclear ribonucleoprotein particles
Bruzik J, Doren K, Hirsh D, Steitz J. Trans splicing involves a novel form of small nuclear ribonucleoprotein particles. Nature 1988, 335: 559-562. PMID: 2971142, DOI: 10.1038/335559a0.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinSL RNACis splicingSplice siteNuclear ribonucleoproteinPrecursor-messenger RNA (pre-mRNA) transcriptsHeLa cell nuclear extractsSame nuclear compartmentTrans-splicing reactionCell nuclear extractsPossible secondary structuresMessenger RNA transcriptsSm snRNPSplice acceptor siteTrans splicingCellular machineryLeader transcriptNuclear compartmentNucleotide sequenceSplicing processRNA transcriptsNuclear extractsSnRNPSplicingSecondary structure
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