2024
Kv1.3 regulation of brain immune function in vitro and in vivo
Bowen C, Nguyen H, Lin Y, Bagchi P, Natu A, Xiao H, Espinosa‐Garcia C, Kumar P, Wulff H, Seyfried N, Rangaraju S. Kv1.3 regulation of brain immune function in vitro and in vivo. Alzheimer's & Dementia 2024, 20: e093042. PMCID: PMC11709668, DOI: 10.1002/alz.093042.Peer-Reviewed Original ResearchProtein processingN-terminusProximity-based proteomicsProtein-protein interactomePDZ-binding motifPDZ-binding domainPlasma membrane proteinsDeletion in vivoJurkat T cellsT cellsKO miceBiotin ligaseCMV-Cre miceRNA-seqTrafficking proteinsBiotinylated proteinsTranscriptome levelC-terminusMembrane proteinsInteractorsMicroglial signatureImmune signalingPlasma membraneLPS stimulationDifferential expression
2023
Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease
Bowen C, Lin Y, Zeng H, Nguyen H, Xiao H, Kumar P, Duong D, Espinosa‐Garcia C, Wulff H, Seyfried N, Rangaraju S. Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078851.Peer-Reviewed Original ResearchKv1.3 blockadeKv1.3 channelsKv1.3 blockersMouse model of AD pathologyBlockade of Kv1.3Immune regulatory roleWestern blottingAlzheimer's diseaseCMV-Cre miceChannels in vitroPDZ-binding motifPlasma membrane proteinsInhibition in vivoCell type-specificPharmacological blockadeOlder 5XFAD miceCMV-CreFunction of microgliaChannel abundanceKv1.3Mouse modelBiotin ligaseFlow cytometryTrafficking proteinsBiotinylated proteins
2022
A pathogenic variant of TULP3 causes renal and hepatic fibrocystic disease
Khamirani H, Palicharla VR, Dastgheib SA, Dianatpour M, Imanieh MH, Tabei SS, Besse W, Mukhopadhyay S, Liem KF. A pathogenic variant of TULP3 causes renal and hepatic fibrocystic disease. Frontiers In Genetics 2022, 13: 1021037. PMID: 36276950, PMCID: PMC9585244, DOI: 10.3389/fgene.2022.1021037.Peer-Reviewed Original ResearchTubby-like protein 3Tubby domainPatient variantsC-terminal tubby domainAssociate with phosphoinositidesRecessive deleterious mutationsMembrane-associated proteinsInner medullary collecting duct 3 cellsDuct 3 cellsHomozygous missense mutationConserved domainsProtein traffickingIntraflagellar transportTrafficking proteinsDeleterious mutationsPatient mutationsCritical residuesMissense mutationsTULP3Pathogenic variantsLike protein 3Phenotypic spectrumMutationsCiliary dysfunctionProtein 3
2017
TRAK2, a novel regulator of ABCA1 expression, cholesterol efflux and HDL biogenesis
Lake N, Taylor R, Trahair H, Harikrishnan K, Curran J, Almeida M, Kulkarni H, Mukhamedova N, Hoang A, Low H, Murphy A, Johnson M, Dyer T, Mahaney M, Göring H, Moses E, Sviridov D, Blangero J, Jowett J, Bozaoglu K. TRAK2, a novel regulator of ABCA1 expression, cholesterol efflux and HDL biogenesis. European Heart Journal 2017, 38: 3579-3587. PMID: 28655204, PMCID: PMC6251650, DOI: 10.1093/eurheartj/ehx315.Peer-Reviewed Original ResearchConceptsNovel regulatorLiver X receptorHDL biogenesisCholesterol effluxTranscription of ABCA1ABCA1 expressionSan Antonio Family Heart StudyAbsence of ABCA1Novel associationsSiRNA-mediated knockdownTrafficking proteinsTranscriptomic dataABCA1 promoterMolecular basisTRAK2RegulatorKnockdownBiogenesisHuman macrophagesMember 1Protein expressionEfflux pathwayX receptorHDL metabolismFamily Heart Study
2015
Chapter 7 RNAi screens of lysosomal trafficking
Garg S, Brenner M. Chapter 7 RNAi screens of lysosomal trafficking. Methods In Cell Biology 2015, 126: 119-138. PMID: 25665444, DOI: 10.1016/bs.mcb.2014.10.030.Peer-Reviewed Original ResearchConceptsShort hairpin RNA screenRNA interference screenStudy lysosome functionCargo traffickingInterference screenTrafficking proteinsGene candidatesMammalian cellsArray screeningRNA screeningLysosomal functionMicrobial killingAssay developmentLysosomesScreening systemPresentation to T cellsAntigen presentation to T cellsRNATraffickingProteinVesiclesScreeningT cellsBiologyCentral position
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