2018
Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography
Park D, Lara-Tejero M, Waxham MN, Li W, Hu B, Galán JE, Liu J. Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography. ELife 2018, 7: e39514. PMID: 30281019, PMCID: PMC6175578, DOI: 10.7554/elife.39514.Peer-Reviewed Original ResearchConceptsCryo-electron tomographyEffector translocationBacterial-host cell contactType III protein secretion systemProtein secretion systemHost cell interfaceProtein secretion machinesCell membraneComplex host-pathogen interactionsType III secretionHost-pathogen interactionsHost cell membraneTarget cell membraneNegative bacterial pathogensTranslocon poreSecretion machineEffector proteinsSecretion systemHost cellsBacterial pathogensCell contactCell interfaceIntimate associationTranslocationBacteria
2013
Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell–mediated cytotoxicity
Tuli A, Thiery J, James A, Michelet X, Sharma M, Garg S, Sanborn K, Orange J, Lieberman J, Brenner M. Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell–mediated cytotoxicity. Molecular Biology Of The Cell 2013, 24: 3721-3735. PMID: 24088571, PMCID: PMC3842998, DOI: 10.1091/mbc.e13-05-0259.Peer-Reviewed Original ResearchConceptsLysosome-related organellesMicrotubule-organizing centerImmune synapseKinesin-1Lytic granulesPolarization of lytic granulesGTP-binding proteinsPull-down approachMovement of lysosomesGranule polarizationLytic granule polarizationKinesin family member 5BNatural killer cell-mediated cytotoxicityNK cell lysatesFormation of immune synapseADP-ribosylationTripartite complexArl8bTarget cell membraneInteraction partnersAnterograde movementCell lysatesTarget cellsMember 5BCell membrane
2009
Cytolytic T cells induce ceramide-rich platforms in target cell membranes to initiate graft-versus-host disease
Rotolo J, Stancevic B, Lu S, Zhang J, Suh D, King C, Kappel L, Murphy G, Liu C, Fuks Z, van den Brink M, Kolesnick R. Cytolytic T cells induce ceramide-rich platforms in target cell membranes to initiate graft-versus-host disease. Blood 2009, 114: 3693-3706. PMID: 19666872, PMCID: PMC2766684, DOI: 10.1182/blood-2008-11-191148.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBone Marrow TransplantationCD8-Positive T-LymphocytesCell MembraneCeramidesCytokinesDisease Models, AnimalFemaleGraft vs Host DiseaseHepatocytesInterferon-gammaIntestine, SmallLiverLymphocyte ActivationMaleMiceMice, Inbred C57BLMice, Inbred MRL lprMice, SCIDSkinSphingomyelin PhosphodiesteraseSurvival RateT-Lymphocytes, CytotoxicConceptsHost diseaseT cellsT cell proliferation/activationAllogeneic bone marrowAcute inflammatory phaseRelevant mouse modelTumor necrosis factorCytolytic T cellsProliferation/activationCytolytic T lymphocytesPotential new targetsHost target cellsTarget cell membraneAcute graftAcute GVHDGVHD progressionCytokine stormOrgan injuryNecrosis factorGVHDInflammatory phaseRelevant graftT lymphocytesMouse modelBone marrow
2001
The Immunological Synapse of CTL Contains a Secretory Domain and Membrane Bridges
Stinchcombe J, Bossi G, Booth S, Griffiths G. The Immunological Synapse of CTL Contains a Secretory Domain and Membrane Bridges. Immunity 2001, 15: 751-761. PMID: 11728337, DOI: 10.1016/s1074-7613(01)00234-5.Peer-Reviewed Original ResearchConceptsImmunological synapseLytic granule secretionPlasma membrane markersTarget cell deathSecretory domainTarget cell membraneProtein organizationCytotoxic T lymphocytesAdhesive ringAdhesion proteinsCell deathMembrane bridgesGranule secretionMembrane markersCell contactMolecule domainsCell membraneFixed cell studiesCellsExocytosisCell studiesMembraneDomainProteinTarget
2000
Methods for Detecting Lytic Granules in Natural Killer Cells
Darmon A, Page L, Griffiths G. Methods for Detecting Lytic Granules in Natural Killer Cells. Methods In Molecular Biology 2000, 121: 145-153. PMID: 10818723, DOI: 10.1385/1-59259-044-6:145.Peer-Reviewed Original ResearchConceptsInitiate cell deathCell death in vivoFormation of membrane poresDeath in vivoApoptotic pathwayTarget cellsAntibody-dependent cellular cytotoxicityTarget cell membraneSecretory granulesCell deathLytic granulesMembrane contactNatural killerNK cellsProteinCell membraneCellsKilling processDestroy target cellsNatural killer cellsNatural cytotoxicityMembraneKiller cellsCellular cytotoxicityGranules
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply