2024
Development of a 31P magnetic resonance spectroscopy technique to quantify NADH and NAD+ at 3 T
Mevenkamp J, Bruls Y, Mancilla R, Grevendonk L, Wildberger J, Brouwers K, Hesselink M, Schrauwen P, Hoeks J, Houtkooper R, Buitinga M, de Graaf R, Lindeboom L, Schrauwen-Hinderling V. Development of a 31P magnetic resonance spectroscopy technique to quantify NADH and NAD+ at 3 T. Nature Communications 2024, 15: 9159. PMID: 39443469, PMCID: PMC11499639, DOI: 10.1038/s41467-024-53292-4.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAdultAgedFemaleHumansMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleMiddle AgedMuscle, SkeletalNADPhantoms, ImagingPhosphorus IsotopesReproducibility of ResultsConceptsPhysically active older adultsActive older adultsMetabolic healthHuman skeletal musclePhosphorous magnetic resonance spectroscopySedentary individualsOlder adultsStimulate mitochondrial biogenesisHealthSkeletal muscleMitochondrial biogenesisNAD+Physiological decreaseNADH contentNADHQuantify NADHClinical 3Magnetic resonance spectroscopy techniquesMR sequencesAdultsMeasurement reproducibility
2021
NMR visibility of deuterium‐labeled liver glycogen in vivo
De Feyter HM, Thomas MA, Behar KL, de Graaf R. NMR visibility of deuterium‐labeled liver glycogen in vivo. Magnetic Resonance In Medicine 2021, 86: 62-68. PMID: 33590529, PMCID: PMC8005460, DOI: 10.1002/mrm.28717.Peer-Reviewed Original ResearchAnimalsDeuteriumGlucoseLiverLiver GlycogenMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMice
2020
In vivo MRS measurement of 2‐hydroxyglutarate in patient‐derived IDH‐mutant xenograft mouse models versus glioma patients
Tiwari V, Mashimo T, An Z, Vemireddy V, Piccirillo S, Askari P, Hulsey K, Zhang S, de Graaf R, Patel T, Pan E, Mickey B, Maher E, Bachoo R, Choi C. In vivo MRS measurement of 2‐hydroxyglutarate in patient‐derived IDH‐mutant xenograft mouse models versus glioma patients. Magnetic Resonance In Medicine 2020, 84: 1152-1160. PMID: 32003035, PMCID: PMC7263951, DOI: 10.1002/mrm.28183.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain NeoplasmsComputer SimulationGliomaGlutaratesHumansIsocitrate DehydrogenaseMagnetic Resonance SpectroscopyMiceNeoplasm TransplantationConceptsPatient-derived xenograftsPatient-derived xenograft micePreclinical modelsGlioma patientsPatient-derived xenograft tumorsPatient-derived xenograft modelsDetection of 2HGIDH1 R132H mutationShort-TE MRSXenograft mouse modelMutant gliomasR132H mutationIDH-mutantIn vivo MRS measurementsMouse modelPhantom analysisMetabolic reprogrammingPatientsMetabolite levelsGliomaMiceMetabolic fingerprintsPRESS sequenceIsocitrate dehydrogenaseMRS methods
2019
In vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer
Rothman DL, de Graaf R, Hyder F, Mason GF, Behar KL, De Feyter HM. In vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer. NMR In Biomedicine 2019, 32: e4172. PMID: 31478594, DOI: 10.1002/nbm.4172.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAnimalsBrain NeoplasmsCarbon IsotopesHumansMagnetic Resonance SpectroscopyMental DisordersNeurotransmitter AgentsSynaptic Transmission
2017
Selective proton‐observed, carbon‐edited (selPOCE) MRS method for measurement of glutamate and glutamine 13C‐labeling in the human frontal cortex
De Feyter H, Herzog RI, Steensma BR, Klomp DWJ, Brown PB, Mason GF, Rothman DL, de Graaf R. Selective proton‐observed, carbon‐edited (selPOCE) MRS method for measurement of glutamate and glutamine 13C‐labeling in the human frontal cortex. Magnetic Resonance In Medicine 2017, 80: 11-20. PMID: 29134686, PMCID: PMC5876108, DOI: 10.1002/mrm.27003.Peer-Reviewed Original Research
2013
Intramolecular zero‐quantum‐coherence 2D NMR spectroscopy of lipids in the human breast at 7 T
de Graaf R, Klomp D, Luijten P, Boer V. Intramolecular zero‐quantum‐coherence 2D NMR spectroscopy of lipids in the human breast at 7 T. Magnetic Resonance In Medicine 2013, 71: 451-457. PMID: 23468435, DOI: 10.1002/mrm.24701.Peer-Reviewed Original ResearchConceptsMagnetic field homogeneityZero-quantum coherencesMagnetic field inhomogeneityField homogeneityField inhomogeneityNavigator echo correctionHuman breastEcho correctionSusceptible boundariesSpectra in vivoHuman breast tissueLipid profileInhomogeneityBreast tissueBreastIn vivoIn vitroLipid signalingSpectraNMR spectraMRSSubjectsNMR
2012
In vivo MRS and histochemistry of status epilepticus‐induced hippocampal pathology in a juvenile model of temporal lobe epilepsy
van der Hel W, van Eijsden P, Bos IW, de Graaf R, Behar KL, van Nieuwenhuizen O, de Graan P, Braun KP. In vivo MRS and histochemistry of status epilepticus‐induced hippocampal pathology in a juvenile model of temporal lobe epilepsy. NMR In Biomedicine 2012, 26: 132-140. PMID: 22806932, DOI: 10.1002/nbm.2828.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCholineEpilepsy, Temporal LobeGamma-Aminobutyric AcidGlutamineHippocampusMagnetic Resonance SpectroscopyMaleNeuronsNeurotransmitter AgentsRatsRats, WistarConceptsTemporal lobe epilepsyStatus epilepticusSynthase immunoreactivityNeuronal lossHippocampal pathologyLobe epilepsyDevelopment of TLECholine-containing compound concentrationsChildhood status epilepticusPre-epileptic stateSpontaneous recurrent seizuresVivo MRSJuvenile rat modelHippocampal injuryChronic epilepsyRecurrent seizuresSpontaneous seizuresNeurotransmitter imbalanceParvalbumin immunoreactivityVivo immunohistochemistryInhibitory neurotransmissionΓ-aminobutyric acidEpileptogenic processMRS findingsNeuronal death
2011
Multislice 1H MRSI of the human brain at 7 T using dynamic B0 and B1 shimming
Boer VO, Klomp DW, Juchem C, Luijten PR, de Graaf RA. Multislice 1H MRSI of the human brain at 7 T using dynamic B0 and B1 shimming. Magnetic Resonance In Medicine 2011, 68: 662-670. PMID: 22162089, PMCID: PMC3306521, DOI: 10.1002/mrm.23288.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsBrain ChemistryHumansMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMolecular ImagingProtonsSensitivity and SpecificityConceptsMagnetic fieldRadio frequency power depositionHigh magnetic fieldsMagnetic field shimmingRadio frequency controlUltra-high fieldMagnetic field inhomogeneityChemical shift resolutionMagnetic field homogeneityField shimmingPower depositionSpectroscopic imaging sequenceField inhomogeneityNew acquisition strategyField homogeneitySpectroscopic imagingShimmingInhomogeneityFieldDynamic B0Nutation angleHigh sensitivityB0ImagingImaging sequence13C MRS studies of neuroenergetics and neurotransmitter cycling in humans
Rothman DL, De Feyter HM, de Graaf R, Mason GF, Behar KL. 13C MRS studies of neuroenergetics and neurotransmitter cycling in humans. NMR In Biomedicine 2011, 24: 943-957. PMID: 21882281, PMCID: PMC3651027, DOI: 10.1002/nbm.1772.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAgingAlzheimer DiseaseAnimalsBrainCarbon IsotopesCitric Acid CycleEnergy MetabolismGlutamic AcidGlutamineHumansMagnetic Resonance SpectroscopyNeuronsNeurotransmitter AgentsConceptsNeurotransmitter cyclingMRS studiesHigh neuronal activityHuman brain diseasesOnly noninvasive methodBrain metabolismGlutamate neurotransmissionNeuronal activityPsychiatric diseasesNeuronal functionBrain diseasesBrain functionClinical researchNeuroenergeticsNoninvasive methodMRSMRS measurementsHuman researchDiseaseBrain statesRecent findingsImportant new informationHumansMetabolic pathwaysNeurotransmission
2009
In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission
Van Eijsden P, Behar KL, Mason GF, Braun KP, De Graaf RA. In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission. Journal Of Neurochemistry 2009, 112: 24-33. PMID: 19818103, PMCID: PMC2843425, DOI: 10.1111/j.1471-4159.2009.06428.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainBrain ChemistryCarbon IsotopesEnergy MetabolismGamma-Aminobutyric AcidGlutamic AcidHydrogenMagnetic Resonance SpectroscopyRatsRats, WistarSynaptic TransmissionConceptsGABAergic neurotransmissionRat brainGlutamatergic/GABAergic neurotransmissionLow GABA concentrationsInhibitory neurotransmissionGlutamatergic neurotransmissionCerebral energeticsGABA concentrationNeurotransmissionBrain functionNeurotransmitter fluxEnergy metabolismNeurochemical profilingVivoBrainHigh magnetic fieldsGABAergicExcitatoryMagnetic fieldIn situ 3D magnetic resonance metabolic imaging of microwave‐irradiated rodent brain: a new tool for metabolomics research
De Graaf RA, Chowdhury GM, Brown PB, Rothman DL, Behar KL. In situ 3D magnetic resonance metabolic imaging of microwave‐irradiated rodent brain: a new tool for metabolomics research. Journal Of Neurochemistry 2009, 109: 494-501. PMID: 19200336, PMCID: PMC2843429, DOI: 10.1111/j.1471-4159.2009.05967.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainImaging, Three-DimensionalMagnetic Resonance SpectroscopyMaleMetabolomicsMicrowavesRadionuclide ImagingRatsRats, Sprague-Dawley
2001
Detection of γ-Aminobutyric Acid (GABA) by Longitudinal Scalar Order Difference Editing
de Graaf R, Rothman D. Detection of γ-Aminobutyric Acid (GABA) by Longitudinal Scalar Order Difference Editing. Journal Of Magnetic Resonance 2001, 152: 124-131. PMID: 11531371, DOI: 10.1006/jmre.2001.2371.Peer-Reviewed Original ResearchDifferentiation of Glucose Transport in Human Brain Gray and White Matter
de Graaf R, Pan J, Telang F, Lee J, Brown P, Novotny E, Hetherington H, Rothman D. Differentiation of Glucose Transport in Human Brain Gray and White Matter. Cerebrovascular And Brain Metabolism Reviews 2001, 21: 483-492. PMID: 11333358, DOI: 10.1097/00004647-200105000-00002.Peer-Reviewed Original ResearchMeSH KeywordsAdultBiological TransportBlood GlucoseBrainFemaleGlucoseHomeostasisHumansHyperglycemiaKineticsMagnetic Resonance SpectroscopyMaleConceptsKinetic transport modelSpectroscopic measurementsStandard modelTransport modelHuman brain grayNuclear magnetic resonance spectroscopic measurementsStandard transport modelMagnetic resonance spectroscopic measurementsThreefold lower rateNuclear magnetic resonance spectroscopyResonance spectroscopyHuman brain gray matterMagnetic resonance spectroscopyMatterVolume compositionSpectroscopyWhite matter levelsMeasurementsDiffusion NMR spectroscopy
Nicolay K, Braun K, de Graaf R, Dijkhuizen R, Kruiskamp M. Diffusion NMR spectroscopy. NMR In Biomedicine 2001, 14: 94-111. PMID: 11320536, DOI: 10.1002/nbm.686.Peer-Reviewed Original ResearchSingle‐shot diffusion trace 1H NMR spectroscopy
de Graaf R, Braun K, Nicolay K. Single‐shot diffusion trace 1H NMR spectroscopy. Magnetic Resonance In Medicine 2001, 45: 741-748. PMID: 11323799, DOI: 10.1002/mrm.1101.Peer-Reviewed Original ResearchAlgorithmsAnimalsAnisotropyBrainBrain MappingImage Processing, Computer-AssistedMagnetic Resonance SpectroscopyMaleRatsRats, Wistar
2000
In Vivo 31P-NMR Diffusion Spectroscopy of ATP and Phosphocreatine in Rat Skeletal Muscle
de Graaf R, van Kranenburg A, Nicolay K. In Vivo 31P-NMR Diffusion Spectroscopy of ATP and Phosphocreatine in Rat Skeletal Muscle. Biophysical Journal 2000, 78: 1657-1664. PMID: 10733948, PMCID: PMC1300762, DOI: 10.1016/s0006-3495(00)76717-8.Peer-Reviewed Original Research
1999
Magnetic coupling of creatine/phosphocreatine protons in rat skeletal muscle, as studied by 1H‐magnetization transfer MRS
Kruiskamp M, de Graaf R, van Vliet G, Nicolay K. Magnetic coupling of creatine/phosphocreatine protons in rat skeletal muscle, as studied by 1H‐magnetization transfer MRS. Magnetic Resonance In Medicine 1999, 42: 665-672. PMID: 10502754, DOI: 10.1002/(sici)1522-2594(199910)42:4<665::aid-mrm7>3.0.co;2-9.Peer-Reviewed Original ResearchAnimalsComputer SimulationCreatineMagnetic Resonance SpectroscopyMaleMuscle, SkeletalPhosphocreatineRatsRats, WistarGlobal HDO uptake in human glioma xenografts is related to the perfused capillary distribution
van der Sanden B, Zandt H, Hoofd L, de Graaf R, Nicolay K, Rijken P, van der Kogel A, Heerschap A. Global HDO uptake in human glioma xenografts is related to the perfused capillary distribution. Magnetic Resonance In Medicine 1999, 42: 479-489. PMID: 10467292, DOI: 10.1002/(sici)1522-2594(199909)42:3<479::aid-mrm10>3.0.co;2-h.Peer-Reviewed Original ResearchOff‐resonance metabolite magnetization transfer measurements on rat brain in situ
de Graaf R, van Kranenburg A, Nicolay K. Off‐resonance metabolite magnetization transfer measurements on rat brain in situ. Magnetic Resonance In Medicine 1999, 41: 1136-1144. PMID: 10371445, DOI: 10.1002/(sici)1522-2594(199906)41:6<1136::aid-mrm9>3.0.co;2-g.Peer-Reviewed Original ResearchAnimalsBrainBrain ChemistryImage EnhancementMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleRatsRats, WistarTime FactorsEffects of Hypoxia-Ischemia and Inhibition of Nitric Oxide Synthase on Cerebral Energy Metabolism in Newborn Piglets
Groenendaal F, De Graaf R, Van Vliet G, Nicolay K. Effects of Hypoxia-Ischemia and Inhibition of Nitric Oxide Synthase on Cerebral Energy Metabolism in Newborn Piglets. Pediatric Research 1999, 45: 827-833. PMID: 10367773, DOI: 10.1203/00006450-199906000-00008.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseCerebral energy metabolismHypoxia-ischemiaCerebral hypoxia-ischemiaN-acetylaspartate ratiosOxide synthaseBaseline valuesNewborn pigletsInorganic phosphate ratioHypoxia groupCerebral energy statusEnergy metabolismSaline 1 hEffect of inhibitionBilateral occlusionNω-nitroCarotid arteryAcetylaspartate ratioL-arginineOld pigletsPhosphate ratioBaseline periodReperfusionNNLAPiglets