Hailong Meng, PhD
Senior Bioinformatics ScientistCards
About
Research
Publications
2026
Single-cell immunophenotyping identifies CD8+GZMK+IFNG+ T cells as a key immune population in cutaneous Lyme disease
Aron E, Meng H, Belperron A, Filippidis P, Dardick K, Kleinstein S, Bockenstedt L. Single-cell immunophenotyping identifies CD8+GZMK+IFNG+ T cells as a key immune population in cutaneous Lyme disease. JCI Insight 2026, 11: e196741. PMID: 41729083, PMCID: PMC12956012, DOI: 10.1172/jci.insight.196741.Peer-Reviewed Original ResearchConceptsEM lesionsT cellsCD4+ T cell subsetsT cell-recruiting chemokinesErythema migransCutaneous Lyme diseaseSkin barrier defenseT cell responsesT cell subsetsCytotoxic gene expressionSkin lesion erythema migransCutaneous immune responsesIFN-regulated genesClinical signsExpression of IFN-regulated genesB cellsUninvolved skinLyme diseaseBarrier defenseImmune populationsIFNG expressionImmune responseInflammatory potentialSingle-cell transcriptomicsEndothelial cells
2025
Multiparameter Profiling Distinguishes Functional Subsets of Platelets in Aging
Montgomery R, Tanguay A, Mohanty S, Shelar A, Rondina M, Meng H, Kleinstein S, Shaw A. Multiparameter Profiling Distinguishes Functional Subsets of Platelets in Aging. Innovation In Aging 2025, 9: igaf122.3627. PMCID: PMC12761676, DOI: 10.1093/geroni/igaf122.3627.Peer-Reviewed Original ResearchExpression of CD62PTLR-induced activationAntibodies to surface markersSurface markersDifferential expression of markersElevated expressionAberrant immune responseAdhesion to endotheliumPlatelet-rich plasmaSubsets of plateletsExpression of markersResponse to stimulationActivation of plateletsImmune agingIL-10Differential expressionCD107bImmune responseFlow cytometryPlatelet aggregationMass cytometryFlowSOM clusteringCD62PPlateletPlatelet populationSequential class switching generates antigen-specific gut IgA from IgG1 B cells
Siniscalco E, Meng H, Gabernet G, Pacheco G, Saghaei S, Ramirez S, Hills L, Faraji F, Chen S, Yin X, Dien C, Hoyt L, Liu E, Barrett A, Briggs N, Williams A, Shukla V, Crotty S, Wesemann D, Kleinstein S, Craft J, Eisenbarth S. Sequential class switching generates antigen-specific gut IgA from IgG1 B cells. Immunity 2025, 58: 3075-3093.e6. PMID: 41253159, PMCID: PMC12834128, DOI: 10.1016/j.immuni.2025.10.022.Peer-Reviewed Original ResearchThis study investigates how gut-specific IgA antibodies are generated from IgG1 B cells through sequential class switching, highlighting a crucial mechanism for designing effective mucosal vaccines.nf-core/airrflow: an adaptive immune receptor repertoire analysis workflow employing the Immcantation framework 9075
Gabernet G, Marquez S, Bjornson R, Peltzer A, Meng H, Aron E, Lee N, Jensen C, Ladd D, Polster M, Hanssen F, Heumos S, Yaari G, Kowarik M, Nahnsen S, Kleinstein S. nf-core/airrflow: an adaptive immune receptor repertoire analysis workflow employing the Immcantation framework 9075. The Journal Of Immunology 2025, 214 DOI: 10.1093/jimmun/vkaf283.2527.Peer-Reviewed Original ResearchSingle Cell Transcriptomics and Immune Profiling Identifies Distinct CD8+ Granzyme K+ T Cell Responses in Cutaneous Lyme Disease 9331
Aron E, Meng H, Filippidis P, Belperron A, Kleinstein S, Bockenstedt L. Single Cell Transcriptomics and Immune Profiling Identifies Distinct CD8+ Granzyme K+ T Cell Responses in Cutaneous Lyme Disease 9331. The Journal Of Immunology 2025, 214 DOI: 10.1093/jimmun/vkaf283.2746.Peer-Reviewed Original ResearchT cell responsesT cell subsetsT cellsErythema migransUninvolved skinEM lesionsT cell-recruiting chemokinesEffector T cell subsetsCD4+ Foxp3Cutaneous Lyme diseaseT-cell receptor sequencingRegulatory T cellsNational Institute of Allergy and Infectious DiseasesSkin lesion erythema migransDominant immune cell typePerivascular lymphocytic infiltrateExpression of IL-10Single cell transcriptomicsImmune cell typesClinical signs of infectionSigns of infectionClinical signsLyme diseaseSingle-cell RNACell transcriptome
2024
nf-core/airrflow: An adaptive immune receptor repertoire analysis workflow employing the Immcantation framework.
Gabernet G, Marquez S, Bjornson R, Peltzer A, Meng H, Aron E, Lee N, Jensen C, Ladd D, Polster M, Hanssen F, Heumos S, Yaari G, Kowarik M, Nahnsen S, Kleinstein S. nf-core/airrflow: An adaptive immune receptor repertoire analysis workflow employing the Immcantation framework. PLOS Computational Biology 2024, 20: e1012265. PMID: 39058741, PMCID: PMC11305553, DOI: 10.1371/journal.pcbi.1012265.Peer-Reviewed Original ResearchImmcantation frameworkAIRR-seqAIRR-seq dataAdaptive immune receptor repertoire sequencingSingle-cell sequencing datasetsClonal inferenceSequencing errorsSequencing datasetsNextflow workflowClonal relationshipReceptor sequencesRepertoire sequencingHigh-throughput processingSequenceImmune challengeAnalysis workflowT-cell receptor sequencingNextflowB cellsSARS-CoV-2Experimental toolResponse to SARS-CoV-2Infectious diseasesMaturation of germinal center B cells after influenza virus vaccination in humans
McIntire K, Meng H, Lin T, Kim W, Moore N, Han J, McMahon M, Wang M, Malladi S, Mohammed B, Zhou J, Schmitz A, Hoehn K, Carreño J, Yellin T, Suessen T, Middleton W, Teefey S, Presti R, Krammer F, Turner J, Ward A, Wilson I, Kleinstein S, Ellebedy A. Maturation of germinal center B cells after influenza virus vaccination in humans. Journal Of Experimental Medicine 2024, 221: e20240668. PMID: 38935072, PMCID: PMC11211068, DOI: 10.1084/jem.20240668.Peer-Reviewed Original ResearchConceptsB cellsInfluenza vaccineGerminal centersAntigen-specific GC B cellsResponse to seasonal influenza vaccinationLong-lived bone marrow plasma cellsResponse to influenza vaccinationBone marrow plasma cellsGerminal center B cellsGC B cell clonesInfluenza virus vaccineMaturation of B cellsMarrow plasma cellsSeasonal influenza vaccineMemory B cellsHemagglutinin (HAB cell clonesGC B cellsInfluenza hemagglutinin (HAH5 HANeedle aspirationLymphoid structuresLymph nodesPlasma cellsGC reaction
2023
High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults
Wang M, Jiang R, Mohanty S, Meng H, Shaw A, Kleinstein S. High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults. Aging 2023, 15: 9250-9274. PMID: 37367734, PMCID: PMC10564424, DOI: 10.18632/aging.204778.Peer-Reviewed Original ResearchConceptsB cellsActivated B cellsB cell receptorOlder adultsInfluenza vaccinationAge groupsPeripheral blood B cellsYoung adultsInactivated influenza vaccineB cell responsesSubstantial disease burdenBlood B cellsMemory B cellsInfluenza vaccination responsesStrong antibody responseAge-related changesInfluenza vaccineVaccination responseSeasonal influenzaAntibody responseHospital visitsDisease burdenSomatic hypermutation frequenciesVaccinationCell responses
2022
The Immune Signatures data resource, a compendium of systems vaccinology datasets
Diray-Arce J, Miller HER, Henrich E, Gerritsen B, Mulè MP, Fourati S, Gygi J, Hagan T, Tomalin L, Rychkov D, Kazmin D, Chawla DG, Meng H, Dunn P, Campbell J, Sarwal M, Tsang J, Levy O, Pulendran B, Sekaly R, Floratos A, Gottardo R, Kleinstein S, Suárez-Fariñas M. The Immune Signatures data resource, a compendium of systems vaccinology datasets. Scientific Data 2022, 9: 635. PMID: 36266291, PMCID: PMC9584267, DOI: 10.1038/s41597-022-01714-7.Peer-Reviewed Original ResearchConceptsHuman Immunology Project ConsortiumDifferent vaccinesCost-effective public health interventionsGene Expression OmnibusInfection-induced morbiditySystems immunology approachPublic health interventionsMultiple relevant studiesVaccine responsesHealth interventionsVaccineRelevant studiesVaccine discoveryUnderstanding of mechanismsMolecular signaturesImmPortSystems immunologyImmunologyStudy dataTraditional immunologyMorbidityCohortImmunogenicityMortalityReleaseMetabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults
Chou C, Mohanty S, Kang HA, Kong L, Avila‐Pacheco J, Joshi SR, Ueda I, Devine L, Raddassi K, Pierce K, Jeanfavre S, Bullock K, Meng H, Clish C, Santori FR, Shaw AC, Xavier RJ. Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults. Aging Cell 2022, 21: e13682. PMID: 35996998, PMCID: PMC9470889, DOI: 10.1111/acel.13682.Peer-Reviewed Original ResearchConceptsInfluenza vaccine responsesInfluenza vaccinationVaccine responsesHigh respondersAntibody responseImmune responseMore effective influenza vaccinesOlder adultsEffective influenza vaccinesSevere respiratory infectionsRobust immune responseLow antibody responseInfluenza vaccineRespiratory infectionsSignificant morbiditySeasonal influenzaInflammatory responseAge-related differencesDay 28Flu seasonOlder subjectsVaccinationHR subjectsMetabolomic signaturePlasma metabolites