Biomedical Engineering; Biotechnology; Cnidaria; Drug Therapy; Electrophysiology; Epilepsy; Neuropharmacology; Neurophysiology; Neurosciences; Optics and Photonics
Cellular & Molecular Physiology: Cell to Cell Communication | Cellular Neurophysiology | Membrane Biophysics | Membrane Proteins - Ion Channels | Neurobiology and Physiology of Synapses | Physiology of Human Disease
Dr Pieribone is developing genetically encoded fluorescent probes of membrane electrical potential. These probes allow one to use optical instruments (microscopes) to monitor the electrical activity of neurons. Such an approach is less invasive, allows study of identified cell types over large regions of the cortical surface. The laboratory has also engineered miniature imaging systems that can be head mounted on mammels and allow mobile recording of neuronal activity. These types of studies will allow a better understanding of the neuronal networks that encode information in the central nervous system.
Specialized Terms: Neurophysiology; Neurotransmission; Voltage and calcium imaging; Sensory physiology; Drug development; Coral biology; Fluorescent proteins
Extensive Research Description
My published research has spanned 26 years (beginning with a Science paper published in 1986 while I was an undergraduate), produced 73 publications in peer-reviewed journals and I co-authored a well received (Nature, Technology or Mixed Signals Blog, CBE Life Science Education, Oceanography, Development, Yale Scientific and Nature Cell Biology), original peer-reviewed lay scientific book (“Aglow in the dark: the revolutionary science of biofluorescence” Harvard Press). My research has spanned a number of scientific areas: catecholamine anatomy and physiology, neuropeptide anatomy and physiology, adrenoceptor distribution in the central nervous system, serotonergic anatomy, the physiology and biochemistry of synaptic transmission, development of genetically-encoded voltage probes, identification of fluorescent proteins in marine animals, and information content in barrel cortex voltage-dye imaging. My work has received some 6,7075 citations, an average of 58 per publication and 181 per year since 1986 (h-index of 39).
As an undergraduate and graduate student I published a series of papers on the afferent control of the locus coeruleus. These publications developed a novel and still held, concept that control of the brain’s main norepinephrine system largely resided in the hind brain. This concept realigned the fields perception of what factors controlled this important brain system. The locus coeruleus/norepinephrine system is involved in the actions of a number of widely used psychopharmacological interventions for depression, mania and schizophrenia.
As a Fogarty and National Science Foundation Fellow at the Nobel Institute of Neurophysiology in Stockholm Sweden, I published a series of studies on the anatomical distribution of the newly cloned adrenoceptors. These studies were the first to accurately identify which cells in the brain (and rest of the body) expressed which adrenoceptors. Previous receptor binding studies could not identify which cells or which cellular elements produced the labeled receptors and the specificity of the labeling was always questionable. These studies remain the most complete mapping of adrenoceptor subtypes in the brain (and other organs). While in Sweden I also examined the neurophysiologic response of monoaminergic neurons to the neuropeptide galanin and nitrous oxide. These represent one of only a handful of studies examining the neurophysiologic effects of galanin.
At The Rockefeller University I developed an in situ experimental preparation of an isolated large synapse for use in biochemical studies of neurotransmitter release. My research focused on the role of synapsin and actin in neurotransmission. We established that acute removal of the synaptic vesicle protein, synapsin from an adult synapses causes little change in low frequency neurotransmitter release but highly impairs high frequency neurotransmitter release. These studies combined with corroborative electron microscopic data, strongly suggested that the role of the most prevalent synaptic vesicle protein known is to create a reserve of synaptic vesicles that can be rapidly mobilized during bouts of high activity. The loss of this control was later shown to produce seizures in adult mice and in humans. At The Pierce Laboratory/Yale University I continued my work on synaptic transmission and moved to study the role of actin and actin- binding proteins on synaptic ultrastructure and physiology. In a series of papers we identified a clear cut role for actin, synapsins and talin in the recycling and clustering of synaptic vesicles.
In 2001 I began a research project to develop a genetically-encoded voltage probe based on green fluorescent protein. Being able to record electrical activity in virtually any specific cell type non-invasively will revolutionize neuroscience. In 2002 we published one of three original proof-of-concept genetically-encoded voltage probes to be developed by the scientific community. Our probe was the first to show rapid on and off kinetics. This publication led to an NIH R21 grants to explore other probes. At that time we also began to look for new fluorescent proteins that would preform better in voltage probes. We have begun a program to clone fluorescent proteins from coral. Our voltage probe became a strong proof-of- concept for the production of such probes. We subsequently received a multicenter (Yale, Riken (Japan), U. Montana, U. Penn. and U.C. Berkeley) NIH U24 grant that involved analyzing a large protein space for improved probes. This grant resulted the discovery and publication from my laboratory in September 2012, of a major advance in optical recording of brain activity (Jin, Lei et al. “Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe.” Neuron 75.5 (2012): 779–785.) This was followed by the first ever fully optical neurophysiology study (Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically targeted optical electrophysiology in intact neural circuits. Cell 154: 904–913, 2013.). This work has received considerable attention in relevant media (BioTechniques, Yale Medicine, Yale Daily News, Science Daily, Scientific American, National Geographic, NIH News, Reddit).
My laboratory has also developed the first head-mountable, high speed, fluorescent microscope system for monitoring electrical activity using genetically-encoded sensors of voltage and calcium. This microscope is now being used in combination with the above mentioned probe to study wide-spread motor cortical activity in an awake behaving animal with high temporal resolution. We are developing a fully optical neural prosthesis capable of informing accurate positional information in realtime. These studies are aimed at eventual human implementation in paralyzed patients.
I have been intimately involved in the development and Phase II/III clinical testing of the neurosteroid ganaxolone and allopregnanolone (I am the author of clinical grant applications, a peer-review paper and clinical protocols). I have co-developed the three clinical trials for these compounds in epilepsy ,post traumatic stress disorder and traumatic brain injury. I consider the development of neuropharmaceuticals a natural extension of my laboratory work at Yale University and the ultimate and most significant application of basic science to the betterment of human health.
I am currently the President and co-founder of Affinimark Technologies, Inc. which is developing a rapid point-of-care test for the presence of cerebrospinal fluid in bodily fluids leaked from the head. No such test exists and would improve patient care following traumatic head injury and sinus/ear surgery.
My scientific achievements extend well beyond my laboratory science. I am a Research Scientist at both the Mystic Aquarium and Center for Exploration in Mystic Connecticut and the American Museum of Natural History in New York City. I have co-curated a permanent exhibit at the Mystic Aquarium (http://www.mysticaquarium.org/animals-and-exhibits/exhibits/indoor/259-fluorescent-corals) and the most popular temporary exhibit to date at the American Museum of Natural History (- http://ez-www.amnh.org/creatures-of-light/ , National Science Foundation story ). For the American Museum of Natural History exhibit we received an National Science Foundation education grant. I have led several research expeditions to study and collect biofluorescent animals from coral reefs in Belize, British Virgin Islands, Malaysia, Australia, Israel, and the Solomon Islands. These have been funded by the National Institutes of Health, National Geographic Society, National Science Foundation, EarthWatch, and the American Museum of Natural History). Accounts of these trips have been documented in televised documentaries, radio interviews (Science and Society, National Public Radio, Studio 360, National Public Radio) and in web (National Geographic, Good News, Big Think, World Science Festival, Live Science) and print articles including The New York Times ( http://scientistatwork.blogs.nytimes.com/author/vincent-pieribone/). In 2010, we (along with my collaborator David Gruber) received an NSF Major Research Instrumentation grant to develop a remotely operated vehicle (ROV) to study deep sea fluorescent and bioluminescent animals.
The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon.
Sparks JS, Schelly RC, Smith WL, Davis MP, Tchernov D, et al. (2014) The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon. PLoS ONE 9: e83259. doi:10.1371/journal.pone.0083259.
Genetically targeted optical electrophysiology in intact neural circuits.
Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, et al. (2013) Genetically targeted optical electrophysiology in intact neural circuits. Cell 154: 904–913.
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics.
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, et al. (2013) Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLoS ONE 8: e81295. doi:10.1371/journal.pone.0081295.
Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals.
Pooyaei Mehr SF, Desalle R, Kao H-T, Narechania A, Han Z, et al. (2013) Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals. Bmc Genomics 14: 546. doi:10.1038/nbt.1754.
A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials.
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T (2012) A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLoS ONE 7: e43454. doi:10.1371/journal.pone.0043454.
Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe.
Jin L, Han Z, Platisa J, Wooltorton JRA, Cohen LB, et al. (2012) Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron 75: 779–785.
Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics.
Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, et al. (2012) Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. J Neurosci Methods 208: 190–196. doi:10.1016/j.jneumeth.2012.05.016.
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells.
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, et al. (2011) Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. J Neurosci Methods 199: 1–9. Available: http://www.sciencedirect.com/science/article/pii/S0165027011002019.
Head-mountable high speed camera for optical neural recording.
Park JH, Platisa J, Verhagen JV, Gautam SH, Osman A, et al. (2011) Head-mountable high speed camera for optical neural recording. J Neurosci Methods 201: 290–295. doi:10.1016/j.jneumeth.2011.06.024.
Effect of high velocity, large amplitude stimuli on the spread of depolarization in S1 “barrel” cortex.
Davis DJ, Sachdev R, Pieribone VA (2011) Effect of high velocity, large amplitude stimuli on the spread of depolarization in S1 “barrel” cortex. Somatosens Mot Res 28: 73–85. doi:10.3109/08990220.2011.613177.
A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms.
Ilagan RP, Rhoades E, Gruber DF, Kao H-T, Pieribone VA, et al. (2010) A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms. FEBS J 277: 1967–1978. doi:10.1111/j.1742-4658.2010.07618.x.
Novel internal regions of fluorescent proteins undergo divergent evolutionary patterns.
Gruber DF, Desalle R, Lienau EK, Tchernov D, Pieribone VA, et al. (2009) Novel internal regions of fluorescent proteins undergo divergent evolutionary patterns. Mol Biol Evol 26: 2841–2848. doi:10.1093/molbev/msp194.
Genetically encoded fluorescent sensors of membrane potential.
Baker BJ, Mutoh H, Dimitrov D, Akemann W, Perron A, Iwamoto Y, Jin L, Cohen LB, Isacoff EY, Pieribone VA, Hughes T, Knöpfel T. Genetically encoded fluorescent sensors of membrane potential. Brain Cell Biol, 36:53-67, 2008.
Patterns of fluorescent protein expression in Scleractinian corals.
Gruber DF, Kao HT, Janoschka S, Tsai J, Pieribone VA. Patterns of fluorescent protein expression in Scleractinian corals. Biol Bulletin, 215:143-54, 2008.
Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus.
Kao HT, Li P, Chao HM, Janoschka S, Pham K, Feng J, McEwen BS, Greengard P, Pieribone VA, Porton B. Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus J Comp Neurol, 507:1860-1870, 2008.
Dynamic regulation of fluorescent proteins from a single species of coral.
Kao HT, Sturgis S, Desalle R, Tsai J, Davis D, Gruber DF, Pieribone VA. Dynamic regulation of fluorescent proteins from a single species of coral. Marine Biotech, 9:733-46, 2007.
Clinical evaluation of ganaxolone in pediatric and adolescent patients with refractory epilepsy.
Pieribone VA, Tsai J, Soufflet C, Rey E, Shaw K, Giller E, Dulac O. Clinical evaluation of ganaxolone in pediatric and adolescent patients with refractory epilepsy. Epilepsia, 48:1870-4, 2007.
In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits.
Nagayama S, Zeng S, Xiong W, Fletcher ML, Masurkar AV, Davis DJ, Pieribone VA, Chen WR. In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits. Neuron, 15:789-803, 2007.
A genetically-targetable fluorescent voltage probe with fast kinetics.
Ataka K and Pieribone VA. A genetically-targetable fluorescent voltage probe with fast kinetics. Biophys J, 82:509-516, 2002.
Midbrain serotonergic neurons are central pH chemoreceptors.
Severson CA, Wang W, Pieribone VA, Dohle CI, Richerson GB. Midbrain serotonergic neurons are central pH chemoreceptors. Nature Neurosci, 6:1139-40, 2003.
Full List of PubMed Publications
- Gruber DF, Loew ER, Deheyn DD, Akkaynak D, Gaffney JP, Smith WL, Davis MP, Stern JH, Pieribone VA, Sparks JS: Biofluorescence in Catsharks (Scyliorhinidae): Fundamental Description and Relevance for Elasmobranch Visual Ecology. Sci Rep. 2016 Apr 25; 2016 Apr 25. PMID: 27109385
- Michaelides EM, Kuang H, Pieribone VA: Dickkopf-related Protein 3 as a Sensitive and Specific Marker for Cerebrospinal Fluid Leaks. Otol Neurotol. 2016 Mar. PMID: 26837002
- Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE: A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. J Neurosci. 2016 Feb 24. PMID: 26911693
- Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA: Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLoS One. 2015; 2015 Nov 11. PMID: 26561348
- Mehr S, Verdes A, DeSalle R, Sparks J, Pieribone V, Gruber DF: Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae). BMC Genomics. 2015 Jun 10; 2015 Jun 10. PMID: 26059236
- Klein M, Afonso B, Vonner AJ, Hernandez-Nunez L, Berck M, Tabone CJ, Kane EA, Pieribone VA, Nitabach MN, Cardona A, Zlatic M, Sprecher SG, Gershow M, Garrity PA, Samuel AD: Sensory determinants of behavioral dynamics in Drosophila thermotaxis. Proc Natl Acad Sci U S A. 2015 Jan 13; 2014 Dec 30. PMID: 25550513
- Han Z, Jin L, Chen F, Loturco JJ, Cohen LB, Bondar A, Lazar J, Pieribone VA: Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight. PLoS One. 2014; 2014 Nov 24. PMID: 25419571
- Sparks JS, Schelly RC, Smith WL, Davis MP, Tchernov D, Pieribone VA, Gruber DF: The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon. PLoS One. 2014; 2014 Jan 8. PMID: 24421880
- Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA: Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics. PLoS One. 2013; 2013 Nov 27. PMID: 24312287
- Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN: Genetically targeted optical electrophysiology in intact neural circuits. Cell. 2013 Aug 15; 2013 Aug 8. PMID: 23932121
- Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA: Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron. 2012 Sep 6. PMID: 22958819
- Park JH, Platisa J, Verhagen JV, Gautam SH, Osman A, Kim D, Pieribone VA, Culurciello E: Head-mountable high speed camera for optical neural recording. J Neurosci Methods. 2011 Oct 15; 2011 Jul 6. PMID: 21763348
- Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T: Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. J Neurosci Methods. 2011 Jul 15; 2011 Apr 8. PMID: 21497167
- Davis DJ, Sachdev R, Pieribone VA: Effect of high velocity, large amplitude stimuli on the spread of depolarization in S1 "barrel" cortex. Somatosens Mot Res. 2011; 2011 Dec 13. PMID: 22150170
- Ilagan RP, Rhoades E, Gruber DF, Kao HT, Pieribone VA, Regan L: A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms. FEBS J. 2010 Apr; 2010 Mar 18. PMID: 20345907
- Nagayama S, Zeng S, Xiong W, Fletcher ML, Masurkar AV, Davis DJ, Pieribone VA, Chen WR: In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits. Neuron. 2007 Mar 15. PMID: 17359915
- Bourne J, Morgan JR, Pieribone VA: Actin polymerization regulates clathrin coat maturation during early stages of synaptic vesicle recycling at lamprey synapses. J Comp Neurol. 2006 Aug 1. PMID: 16739194
- Morgan JR, Di Paolo G, Werner H, Shchedrina VA, Pypaert M, Pieribone VA, De Camilli P: A role for talin in presynaptic function. J Cell Biol. 2004 Oct 11. PMID: 15479735
- Chen Y, Bourne J, Pieribone VA, Fitzsimonds RM: The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity. Neuroreport. 2004 Apr 9. PMID: 15073524
- Severson CA, Wang W, Pieribone VA, Dohle CI, Richerson GB: Midbrain serotonergic neurons are central pH chemoreceptors. Nat Neurosci. 2003 Nov; 2003 Sep 28. PMID: 14517544
- Bloom O, Evergren E, Tomilin N, Kjaerulff O, Löw P, Brodin L, Pieribone VA, Greengard P, Shupliakov O: Colocalization of synapsin and actin during synaptic vesicle recycling. J Cell Biol. 2003 May 26; 2003 May 19. PMID: 12756235
- Pieribone VA, Porton B, Rendon B, Feng J, Greengard P, Kao HT: Expression of synapsin III in nerve terminals and neurogenic regions of the adult brain. J Comp Neurol. 2002 Dec 9. PMID: 12412137
- Ataka K, Pieribone VA: A genetically targetable fluorescent probe of channel gating with rapid kinetics. Biophys J. 2002 Jan. PMID: 11751337