Cell Biology; Epithelial Cells; Kidney; Polycystic Kidney Diseases; Physiology; Ion Pumps
Cellular & Molecular Physiology: Epithelial Transport of Ions and Solutes | Membrane Protein Sorting and Trafficking | Membrane Proteins - Pumps and Transporters | Organ Physiology | Physiology of Human Disease
Center for Polycystic Kidney Disease Research
The surface membranes of epithelial cells are divided into domains characterized by dramatically different protein compositions. Membrane proteins whose distributions are restricted to one of these domains must incorporate information that specifies their appropriate destinations. We seek to determine how this information is encoded and how it is interpreted.
Our studies of cellular trafficking focus on proteins involved in ion transport, as well as on the proteins associated with polycystic kidney disease. Polycystic kidney disease is caused by mutations in genes encoding polycystin-1 and 2. We have found that polycystin-1 undergoes a proteolytic cleavage that releases its cytoplasmic C terminal tail.
This fragment is transported to the nucleus, where it appears to modulate several signaling pathways. This behavior may account for the capacity of polycystin-1 to participate in communication between the cell surface and the nucleus.
Specialized Terms: Ion pumps in polarized epithelia; Sorting and function
Extensive Research Description
Work in the Caplan laboratory is focused on understanding how membrane proteins are sorted to the appropriate cell surface domains of polarized epithelial cells. One of the proteins whose trafficking we study is the Na,K-ATPase, or sodium pump, which generates the ion gradients responsible for most fluid and electrolyte transport processes in the kidney. The Na,K-ATPase must be restricted to the basolateral surfaces of renal tubule epithelial cells. Much remains to be learned about the partner proteins and trafficking pathways that determine the sodium pump’s subcellular distribution and modulate its activity. We have adapted a novel labeling methodology to investigate the attributes of temporally defined cohorts of Na,K-ATPase.
We can observe directly the trafficking itinerary pursued by newly synthesized Na,K-ATPase and isolate newly synthesized Na,K-ATPase in association with its collections of partner proteins. We find that the basolateral delivery of newly synthesized Na,K-ATPase occurs via a pathway distinct from that pursued by other basolateral membrane proteins. We have also detected interactions between the Na,K-ATPase a-subunit and a collection of novel partner proteins that may govern the pump’s trafficking properties. Thus, we have developed tools that permit us to evaluate the trafficking pathways and partner proteins that govern the post-synthetic sorting and regulation of the epithelial Na,K-ATPase.
We also study a common genetic disease that dramatically alters the structure and function of polarized epithelial cells. In Autosomal Dominant Polycystic Kidney Disease (ADPKD) the normal architecture of the kidney tubules is replaced by large fluid filled cysts, which can ultimately result in renal failure. ADPKD is caused by mutations in the PKD1 or PKD2 genes, which encode the polycystin-1 and polycystin-2 proteins, respectively. Both of these proteins are targeted to cilia in polarized epithelial cells. We have found that polycystin-1 undergoes such a proteolytic cleavage that releases its C-terminal tail (CTT), which enters the nucleus and initiates signaling processes. The cleavage occurs in vivo in association with alterations in mechanical stimuli that may be communicated by signaling through the cilium. The C-terminal tail fragment of polycystin-1 participates in a complex with ß-catenin and acts to profoundly inhibit canonical ß-catenin-dependent Wnt signaling. The polycystin-1 C-terminal tail fragment also appears to modulate gene expression, and may induce expression of cilia-related proteins in renal epithelial cells.
We find that all of the signal transduction machinery found in the cilia of olfactory epithelial cells is present in renal epithelial cells. Our data suggest that olfactory receptors and proteins involved in olfactory signal transduction may play a role in regulating renal flow or transport in response to chemosensory cues.
- Chauvet V, et al. Mechanical stimuli induce the cleavage and nuclear translocation of the polycystin-1 C-terminus. J Clin Invest, 114:1433-1443, 2004.
- Zhang L, Li J, Young LH, Caplan MJ. AMP-activated protein kinase regulates the assembly of epithelial tight junctions. Proc Natl Acad Sci USA, 103:17272-17277, 2006.
- Kamsteeg EJ, Duffield AS, Konings IBM, Spencer J, Pagel P, Deen PMT, Caplan MJ. MAL decreases the internalization of the aquaporin-2 water channel. Proc Nat Acad Sci USA, 104:16696-16701, 2007.
- Lal M, Song X, Pluznick JL, Di Giovanni V, Merrick DM, Rosenblum ND, Chauvet V, Gottardi CJ, Pei Y, Caplan MJ. Polycystin-1 C-terminal tail associates with β-catenin and inhibits canonical Wnt signaling. Hum Mol Gen, 17:3105-3117, 2008.
- Pluznick JL, Zou DJ, Zhang X, Yan Q, Rodriguez-Gil DJ, Eisner C, Wells E, Greer CA, Wang T, Firestein S, Schnermann J, Caplan MJ. Functional expression of the olfactory signaling system in the kidney. Proc Nat Acad Sci, 106:2059-2064, 2009.
- Farr GA, Hull M, Mellman IS, Caplan MJ. Membrane proteins follow multiple trafficking pathways to the basolateral cell surface in polarized epithelial cells. J Cell Biol, 186:269-282, 2009.
- Stoops, E.H., M. Hull, C. Olesen, K. Mistry, J.L. Harder, F. Rivera-Molina, D. Toomre, and M.J. Caplan. The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135. J. Cell Biol. 211:287-294, 2015.
- Farr, G.A., M. Hull, E.H. Stoops, R. Bateson and M.J. Caplan. Dual Pulse Chase Microscopy Reveals Early Divergence in the Biosynthetic Trafficking of the Na, K-ATPase and E-cadherin. Mol. Biol. Cell, 26:4401-4411, 2015.
- Pluznick, J.L., R.J. Protzko, H. Gevorgyan, Z. Peterlin, A. Sipos, J. Han, I. Brunet, L.-X. Wan, F.E. Rey, T. Wang, S. Firestein, M. Yanagisawa, J.I. Gordon, A. Eichmann, J. Peti-Peterdi, M.J. Caplan. An Olfactory Receptor Responding to Gut Microbiota-Derived Signals Plays a Role in Renin Secretion and Blood Pressure Regulation. Proc. Nat. Acad. Sci. 110:4410-4415, 2013.
- Merrick, D.M., H.C. Chapin, Z. Yu, S. Somlo, J. Hogenesch, and M.J. Caplan. The Gamma-secretase cleavage product of Polycystin-1 regulates Tcf and CHOP-mediated transcriptional activation through a p300-dependent mechanism. Dev. Cell, 22, 197–210, 2012.