2022
Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy
Khalilimeybodi A, Riaz M, Campbell S, Omens J, McCulloch A, Qyang Y, Saucerman J. Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy. Journal Of Molecular And Cellular Cardiology 2022, 174: 1-14. PMID: 36370475, PMCID: PMC10230857, DOI: 10.1016/j.yjmcc.2022.10.006.Peer-Reviewed Original Research
2019
Sarcomere-Directed Calcium Reporters in Cardiomyocytes
Campbell SG, Qyang Y, Hinson JT. Sarcomere-Directed Calcium Reporters in Cardiomyocytes. Circulation Research 2019, 124: 1151-1153. PMID: 30973804, PMCID: PMC6527368, DOI: 10.1161/circresaha.119.314877.Peer-Reviewed Original Research
2011
High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells
Lee MY, Bozkulak E, Schliffke S, Amos PJ, Ren Y, Ge X, Ehrlich BE, Qyang Y. High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells. Biochemical And Biophysical Research Communications 2011, 416: 51-57. PMID: 22079290, PMCID: PMC3237870, DOI: 10.1016/j.bbrc.2011.10.140.Peer-Reviewed Original ResearchConceptsMurine embryonic stem cellsEmbryonic stem cellsBone morphogenetic proteinEB culturesCardiac differentiationCardiomyocyte productionEmbryoid body culturesStem cellsCardiac-specific genesEarly cardiac developmentCardiogenic growth factorsNK2 transcription factorCardiac regenerative medicineMESC linesTranscription factorsCardiac developmentMorphogenetic proteinsEmbryoid bodiesDifferentiation systemDifferentiation periodFunctional cardiomyocytesHigh-density culturesMyosin light chain 2vRegenerative medicineCell-based therapiesDerivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells
Lee MY, Sun B, Schliffke S, Yue Z, Ye M, Paavola J, Bozkulak EC, Amos PJ, Ren Y, Ju R, Jung YW, Ge X, Yue L, Ehrlich BE, Qyang Y. Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells. Stem Cell Research 2011, 8: 49-57. PMID: 22099020, PMCID: PMC3222859, DOI: 10.1016/j.scr.2011.08.004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdherens JunctionsAnimalsBacterial ProteinsBase SequenceCadherinsCalciumCell Culture TechniquesCell LineCell SeparationConnexin 43Embryonic Stem CellsFlow CytometryGap JunctionsHeart VentriclesImaging, Three-DimensionalIntegrasesLuminescent ProteinsMiceMyocytes, CardiacMyosin Light ChainsPromoter Regions, GeneticProteinsRNA, UntranslatedConceptsVentricular cardiomyocytesCalcium transientsNeonatal mouse ventricular cardiomyocytesFunctional excitation-contraction couplingCardiac contractile performanceDouble transgenic miceCurrent-clamp recordingsIntracellular calcium transientsExcitation-contraction couplingAction potential characteristicsMouse ventricular cardiomyocytesMyosin light chain 2vFluorescence-activated cell sortingAdrenergic signalingIntracellular calciumContractile performanceClamp recordingsTransgenic miceElectrical stimulationCardiac repairInduction of differentiationIsoproterenol stimulationExpression of YFPMouse linesN-cadherinSmall molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells
Ren Y, Lee MY, Schliffke S, Paavola J, Amos PJ, Ge X, Ye M, Zhu S, Senyei G, Lum L, Ehrlich BE, Qyang Y. Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells. Journal Of Molecular And Cellular Cardiology 2011, 51: 280-287. PMID: 21569778, PMCID: PMC3334336, DOI: 10.1016/j.yjmcc.2011.04.012.Peer-Reviewed Original ResearchConceptsHuman iPS cellsAction potential durationHuman iPS cell-derived cardiomyocytesSmall-molecule Wnt inhibitorsIPS cellsPluripotent stem cellsIPS cell-derived cardiomyocytesBMP-4Cardiac disease mechanismsCardiac differentiationCell-derived cardiomyocytesPotential durationBone morphogenetic protein 4Stem cellsWnt inhibitorsCardiomyocyte action potential durationHuman pluripotent stem cellsInduced pluripotent stem cellsHuman induced pluripotent stem cellsDisease mechanismsTranslational cardiac researchIntracellular calcium imagingEfficient cardiac differentiationWnt/β-cateninProduction of cardiomyocytes