Core Resources

Our fish facility is currently being expanded with a target capacity of 1500 tanks. It is staffed and monitored by YARC and users are not required to feed fish (though we welcome users to take an active role in monitoring their fish stocks). Tank per diem is $1.05. For access to the fish facility contact Andrew Prendergast. Prospective users will need to complete YARC Facility training through Part 2: https://medicine.yale.edu/yarc/start/ and schedule a tour with YARC staff.

Injector

Our injector is a WPI Pneumatic PV820 PicoPump mounted on an Olympus SZX7 stereomicroscope. We also have all the accessory equipment necessary to introduce synthesized mRNA or and/or recombinant DNA into zebrafish embryos. Following a training session, this equipment is available to the entire Yale community.

Screening fluorescent microscope

Our screening scope is an Olympus MVX10 equipped with 1.0X and 2.0X apochromatic objectives, GFP/mCherry filter sets, and an Olympus DP74 color CMOS camera. It is excellent for routine macro imaging of live or fixed tissue. Following a training session, this equipment is available to the entire Yale community.

Molecular biology suite

Our molecular biology space is equipped with everything required to generate recombinant DNA, perform routine immunohistochemistry or in situ hybridization, and genotype zebrafish. Use of the space can be negotiated if users do not have access to a suitable molecular biology space of their own.

Behavioral apparatus

We have a ViewPoint ZebraBox Revo 4th Gen. behavioral chamber with the following added modules--

• EZBTOP high intensity light stimulator
• EZVIB vibration module (for escapes, etc.)
• EZBOG2 optogenetics module (for blue light channelrhodopsin activation)

This unit can be adapted for all standard micro plates (6, 12, 24, 48, 96) as well as petri dishes. It is commonly used in studies of circadian behavior.

We are available to train new users in fish techniques as well as for in-person or remote consultations on prospective fish projects over Zoom. To schedule a meeting, email Andrew Prendergast.