Services Offered
We offer the following services. These rates will go into effect 8/1/2025
To place a service request, please fill out our Qualtrics form.
All equipment and services are located in Sterling Hall of Medicine IG43.
Service | Internal Rate | External Rate For Profit | External Rate Non-Profit |
|---|---|---|---|
Making a New Fish Line: CRISPR-Cas9 allele or transgenic line (Tol2, phiC31-Integrase). Includes design, F1 generation, confirming genotyping of the new line; deliverable is one frameshift allele/transgenic line; additional frameshit alleles/transgenic lines are available at a discount rate. | $8,500 | $13,000 | |
Making a New Fish Line: CRISPR-Cas9 homology directed repair allele. Includes design, F1 generation, confirming genotyping of the new line; deliverable is one allele. | $12,500 | $19,500 | |
Phenotyping Group 1: Plasmid/mRNA/CRISPR-Cas9 (Select One) injection to generate F0 data for phenotype screening. Included: 1 experimental condition, 1 control, and 1 assay. 50% discount on additional experimental conditions (max 3) if same control is valid. | $1,250 | $2,000 | |
Phenotyping Group 2: Immunohistochemistry, including sample preparation and imaging up to 12 samples; In-situ hybridization, including riboprobe synthesis, staining and imaging up to 12 samples; In-situ hybridization, including staining and imaging up to 12 samples (Select One) | $645 | $1,150 | |
Phenotyping: Imaging up to 10 fish, including analysis | $525 | $900 | |
Behavioral Phenotyping: per session up to 4 hr; >4 hr experiment will be a discount rate | $200 | ||
Phenotyping: Monitoring up to 10 tanks for a month, including breeding for F2 generations | $200 | $400 | |
Genotyping: including tissue harvest and PCR | $35 | $225 | |
In-vitro Fertilization: Rescuing fish lines | $300 | ||
Basic Training: 2 hr session | $400 | ||
Consultation: Project feasibility; initial hour free | $250 | $475 | |
Protocol, Grant, and Manuscript Assistance | $650 | ||
Using Zebrafish Core Equipment Unassisted | $75 | ||
Cryopreservation: Per fish line | $500 | $800 | |
Molecular Cloning: One-step LR gateway reaction. 50% discount on each construct performed at the same time (limit 4) | $500 | $750 | |
Molecular Cloning: Two-step cloning (pENTR5’ or pME + LR gateway reaction). Customer must provide template material for pENTR5’, pME (or will be a passthrough charge for synthesizing) (limit 4) | $1,000 | $1,700 | |
Staff Service at Hourly Rate | $59.52 |