Featured Publications
PLCγ1 promotes phase separation of T cell signaling components
Zeng L, Palaia I, Šarić A, Su X. PLCγ1 promotes phase separation of T cell signaling components. Journal Of Cell Biology 2021, 220: e202009154. PMID: 33929486, PMCID: PMC8094118, DOI: 10.1083/jcb.202009154.Peer-Reviewed Original ResearchConceptsTCR signal transductionT-cell receptor pathwayLiquid-like condensatesKey adaptor proteinLAT clusteringLAT complexSH2 domainAdaptor proteinPhosphatase CD45Signal transductionTCR pathwayPhospholipase Cγ1ERK activationProtein compositionBiophysical principlesPLCγ1Critical functionsReceptor pathwayPathwayLATMajor componentT cellsCellsActivationDephosphorylationRewired signaling network in T cells expressing the chimeric antigen receptor (CAR)
Dong R, Libby KA, Blaeschke F, Fuchs W, Marson A, Vale RD, Su X. Rewired signaling network in T cells expressing the chimeric antigen receptor (CAR). The EMBO Journal 2020, 39: embj2020104730. PMID: 32643825, PMCID: PMC7429742, DOI: 10.15252/embj.2020104730.Peer-Reviewed Original ResearchConceptsCentral supramolecular activation clusterEssential scaffold proteinT cell receptorSupramolecular activation clusterImmunological synapse formationAntigen receptorActin remodelingScaffold proteinMicrocluster formationDownstream signalingTCR signalingT cell activationSynapse formationCancer cellsSpecific cancer cellsCell receptorSignalingCAR activationT cellsCell activationCellsCAR-T cell activationNative T cellsPathwayActivation clusters
2021
Phase separation in immune signalling
Xiao Q, McAtee CK, Su X. Phase separation in immune signalling. Nature Reviews Immunology 2021, 22: 188-199. PMID: 34230650, PMCID: PMC9674404, DOI: 10.1038/s41577-021-00572-5.Peer-Reviewed Original ResearchConceptsGene I proteinImmune Signaling PathwaysCyclic GMP-AMP synthaseSubstantial conformational changesNew biophysical principleGMP-AMP synthaseCell receptorB cell receptorCytosolic eventsSignal transductionImmune signalingSignaling pathwaysI proteinConformational changesLigand engagementDownstream adaptorsInterferon genesImmune receptorsBiophysical principlesLiquid-liquid phase separationFunctional consequencesT cell receptorPathogenic stimuliSpatial reorganizationOutstanding questions
2020
Surfing on Membrane Waves: Microvilli, Curved Membranes, and Immune Signaling
Orbach R, Su X. Surfing on Membrane Waves: Microvilli, Curved Membranes, and Immune Signaling. Frontiers In Immunology 2020, 11: 2187. PMID: 33013920, PMCID: PMC7516127, DOI: 10.3389/fimmu.2020.02187.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsCarrier ProteinsCell LineCell MembraneCell ShapeCyclodextrinsCytokinesGlycocalyxHumansLymphocyte ActivationMembrane LipidsMembrane ProteinsMiceMicrofilament ProteinsMicroscopy, Electron, ScanningMicrovilliReceptors, Antigen, T-CellSignal TransductionStress, MechanicalSurface PropertiesSynaptosomesT-LymphocytesConceptsFunctional consequencesFinger-like membrane protrusionsT cell signalingSuper-resolution microscopyLocal membrane curvatureActin cytoskeletonMembrane protrusionsSignal transductionCell signalingMembrane curvatureCurved membranesImmune signalingBiochemical activityUnique compartmentLymphocyte microvilliMicrovillus formationCell typesLocal membraneCytoskeletonSignalingMicrovilliMembraneBody of evidenceMembrane wavesImportant roleImaging Chimeric Antigen Receptor (CAR) Activation
Libby KA, Su X. Imaging Chimeric Antigen Receptor (CAR) Activation. Methods In Molecular Biology 2020, 2111: 153-160. PMID: 31933206, DOI: 10.1007/978-1-0716-0266-9_13.Peer-Reviewed Original Research
2019
A composition-dependent molecular clutch between T cell signaling condensates and actin
Ditlev JA, Vega AR, Köster DV, Su X, Tani T, Lakoduk AM, Vale RD, Mayor S, Jaqaman K, Rosen MK. A composition-dependent molecular clutch between T cell signaling condensates and actin. ELife 2019, 8: e42695. PMID: 31268421, PMCID: PMC6624021, DOI: 10.7554/elife.42695.Peer-Reviewed Original Research
2016
Phase separation of signaling molecules promotes T cell receptor signal transduction
Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton J, Rosen MK, Vale RD. Phase separation of signaling molecules promotes T cell receptor signal transduction. Science 2016, 352: 595-599. PMID: 27056844, PMCID: PMC4892427, DOI: 10.1126/science.aad9964.Peer-Reviewed Original ResearchConceptsT cell receptor (TCR) signal transductionHuman Jurkat T cellsActin filament assemblySubmicrometer-sized clustersReceptor signal transductionT cell receptor activationProtein phase separationJurkat T cellsCell receptor activationCell surface receptorsTCR phosphorylationActin regulatorsActin assemblySignal transductionFilament assemblyBiochemical compartmentsFunctional consequencesSurface receptorsModel membranesReceptor activationAssemblyActivationTransductionKinasePhosphorylation
2012
Move in for the kill: motile microtubule regulators
Su X, Ohi R, Pellman D. Move in for the kill: motile microtubule regulators. Trends In Cell Biology 2012, 22: 567-575. PMID: 22959403, PMCID: PMC3482944, DOI: 10.1016/j.tcb.2012.08.003.Peer-Reviewed Original Research
2011
Mechanisms Underlying the Dual-Mode Regulation of Microtubule Dynamics by Kip3/Kinesin-8
Su X, Qiu W, Gupta ML, Pereira-Leal JB, Reck-Peterson SL, Pellman D. Mechanisms Underlying the Dual-Mode Regulation of Microtubule Dynamics by Kip3/Kinesin-8. Molecular Cell 2011, 43: 751-763. PMID: 21884976, PMCID: PMC3181003, DOI: 10.1016/j.molcel.2011.06.027.Peer-Reviewed Original Research