2017
miR-125b promotes MLL-AF9–driven murine acute myeloid leukemia involving a VEGFA-mediated non–cell-intrinsic mechanism
Liu J, Guo B, Chen Z, Wang N, Iacovino M, Cheng J, Roden C, Pan W, Khan S, Chen S, Kyba M, Fan R, Guo S, Lu J. miR-125b promotes MLL-AF9–driven murine acute myeloid leukemia involving a VEGFA-mediated non–cell-intrinsic mechanism. Blood 2017, 129: 1491-1502. PMID: 28053194, PMCID: PMC5356452, DOI: 10.1182/blood-2016-06-721027.Peer-Reviewed Original Research
2016
A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
Cheng J, Roden CA, Pan W, Zhu S, Baccei A, Pan X, Jiang T, Kluger Y, Weissman SM, Guo S, Flavell RA, Ding Y, Lu J. A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions. Nature Communications 2016, 7: 11178. PMID: 27025950, PMCID: PMC4820989, DOI: 10.1038/ncomms11178.Peer-Reviewed Original ResearchAnimalsBacterial ProteinsCell LineChromosome MappingCloning, MolecularClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated Protein 9DNADNA Restriction EnzymesEndonucleasesGene LibraryGenomeHumansMiceMicroRNAsOligonucleotide Array Sequence AnalysisRNA, Guide, CRISPR-Cas SystemsUntranslated Regions
2013
An Extensive Network of TET2-Targeting MicroRNAs Regulates Malignant Hematopoiesis
Cheng J, Guo S, Chen S, Mastriano SJ, Liu C, D’Alessio A, Hysolli E, Guo Y, Yao H, Megyola CM, Li D, Liu J, Pan W, Roden CA, Zhou XL, Heydari K, Chen J, Park IH, Ding Y, Zhang Y, Lu J. An Extensive Network of TET2-Targeting MicroRNAs Regulates Malignant Hematopoiesis. Cell Reports 2013, 5: 471-481. PMID: 24120864, PMCID: PMC3834864, DOI: 10.1016/j.celrep.2013.08.050.Peer-Reviewed Original ResearchConceptsKey tumor suppressorMyeloid differentiation biasTET2 expressionTranslocation 2 (TET2) geneMolecular regulationDifferentiation biasHematopoietic malignanciesTen-ElevenMalignant hematopoiesisTumor suppressorHematopoietic expansionActivity screenMiR-7MiRNAsExpression of TET2Normal hematopoiesisOncogenic potentialTET2Important pathogenic mechanismMiR-101Extensive roleMiR-29cHematopoiesisExpressionRegulation
2012
An In Vivo Functional Screen Uncovers miR-150-Mediated Regulation of Hematopoietic Injury Response
Adams BD, Guo S, Bai H, Guo Y, Megyola CM, Cheng J, Heydari K, Xiao C, Reddy EP, Lu J. An In Vivo Functional Screen Uncovers miR-150-Mediated Regulation of Hematopoietic Injury Response. Cell Reports 2012, 2: 1048-1060. PMID: 23084747, PMCID: PMC3487471, DOI: 10.1016/j.celrep.2012.09.014.Peer-Reviewed Original ResearchConceptsMiR-150Injury responseBone marrow transplant modelCareful clinical managementHematopoietic suppressionTransplant modelPeripheral bloodHematopoietic recoveryRecipient miceClinical managementAssociated impairmentRole of microRNAsMyeloid cellsHeterozygous knockoutProgenitor cellsClonogenic potentialMajor blood lineagesNormal tissue physiologyHematopoietic stemTissue physiologyC-MybTreatmentMicroRNAsFunction screenCellsComplex oncogene dependence in microRNA-125a–induced myeloproliferative neoplasms
Guo S, Bai H, Megyola CM, Halene S, Krause DS, Scadden DT, Lu J. Complex oncogene dependence in microRNA-125a–induced myeloproliferative neoplasms. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 16636-16641. PMID: 23012470, PMCID: PMC3478612, DOI: 10.1073/pnas.1213196109.Peer-Reviewed Original ResearchAnimalsBone Marrow CellsBone Marrow NeoplasmsBone Marrow TransplantationCell LineColony-Forming Units AssayDoxycyclineFlow CytometryGene Expression Regulation, NeoplasticGranulocyte-Macrophage Colony-Stimulating FactorInterleukin-3Leukocytes, MononuclearMiceMice, Inbred C57BLMicroRNAsMyeloproliferative DisordersOncogenesReverse Transcriptase Polymerase Chain Reaction
2010
A microRNA regulating adult hematopoietic stem cells
Guo S, Scadden DT. A microRNA regulating adult hematopoietic stem cells. Cell Cycle 2010, 9: 3637-3638. PMID: 20855952, DOI: 10.4161/cc.9.18.13174.Peer-Reviewed Original ResearchMicroRNA miR-125a controls hematopoietic stem cell number
Guo S, Lu J, Schlanger R, Zhang H, Wang JY, Fox MC, Purton LE, Fleming HH, Cobb B, Merkenschlager M, Golub TR, Scadden DT. MicroRNA miR-125a controls hematopoietic stem cell number. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 14229-14234. PMID: 20616003, PMCID: PMC2922532, DOI: 10.1073/pnas.0913574107.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsStem cell pool sizeStem cell stateLong-term hematopoietic stem cellsCell-autonomous mannerStem cellsStem cell populationCell pool sizeMiR-125aStem cell numbersHematopoietic stem cell numbersEnzyme DicerImmature hematopoietic progenitorsHematopoietic differentiationMutant animalsCell statesProgenitor cell apoptosisMicroRNA processing enzyme DicerMicroRNA clusterProapoptotic genesHSPC populationsHematopoietic expansionSpecific microRNAsUnique microRNAsHSPC apoptosis
2008
MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors
Lu J, Guo S, Ebert BL, Zhang H, Peng X, Bosco J, Pretz J, Schlanger R, Wang JY, Mak RH, Dombkowski DM, Preffer FI, Scadden DT, Golub TR. MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors. Developmental Cell 2008, 14: 843-853. PMID: 18539114, PMCID: PMC2688789, DOI: 10.1016/j.devcel.2008.03.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Bone Marrow CellsCell DifferentiationCell LineageCells, CulturedErythroid CellsErythropoietinGene Expression RegulationGenes, ReporterHematopoietic Stem CellsHumansIntegrin beta3K562 CellsMegakaryocytesMiceMice, Inbred C57BLMicroRNAsModels, BiologicalPlatelet Membrane Glycoprotein IIbProto-Oncogene Proteins c-mybThrombopoietinConceptsMegakaryocyte-erythrocyte progenitorsLineage specificationTranscription factor MYBMiR-150Cell fateLineage fateRegenerative biologyErythroid cellsFunction experimentsMultipotent cellsMegakaryocytic lineageMiRNA expressionPrimary cellsCritical targetModel systemMicroRNAsProgenitorsFateRegulationCellsImportant participantsMYBLineagesMiRNAsBiology