Featured Publications
An Activity‐Based Probe for Studying Crosslinking in Live Bacteria
Gautam S, Kim T, Shoda T, Sen S, Deep D, Luthra R, Ferreira M, Pinho M, Spiegel D. An Activity‐Based Probe for Studying Crosslinking in Live Bacteria. Angewandte Chemie 2015, 127: 10638-10642. DOI: 10.1002/ange.201503869.Peer-Reviewed Original ResearchWall teichoic acidActivity-based probesSingle PBPLive bacteriaAntibiotic targetsCell wallFluorescent mimicTeichoic acidNatural substrateLive cellsEssential processRelative quantitationNatural crosslinksPeptidoglycanBacterial growthSpatiotemporal aspectsBacteriaExperimental toolPBPPeptide mimicsMimics
2020
Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking
Gautam S, Kim T, Howell R, Spiegel DA. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking. Methods In Enzymology 2020, 638: 57-67. PMID: 32416921, DOI: 10.1016/bs.mie.2020.02.016.Peer-Reviewed Case Reports and Technical NotesConceptsBacterial cell wall synthesisSynthetic probesPeptide mimicsPeptidoglycan crosslinkingSynthesisSitu probeKey stepCrosslinkingNumerous classesMechanical strengthNew antibioticsProbeCell wall synthesisPeptidoglycan synthesisSuper-resolution microscopyLive bacteriaWall synthesisReactionCell wallDetailed protocolMicroscopyStaphylococcus aureusHuman pathogens