2003
Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD
Thomson RB, Mentone S, Kim R, Earle K, Delpire E, Somlo S, Aronson PS. Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. American Journal Of Physiology. Renal Physiology 2003, 285: f870-f880. PMID: 12851251, DOI: 10.1152/ajprenal.00153.2003.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsCadherinsDisease Models, AnimalEpitheliumGene SilencingImmunohistochemistryKidneyMiceMicroscopy, ElectronMicroscopy, Electron, ScanningPolycystic Kidney, Autosomal DominantProtein Kinase D2Protein KinasesSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseSolute Carrier Family 12, Member 2Tissue DistributionConceptsCell polarityKsp-cadherinBasolateral membraneRenal cystic epitheliaEpithelial cell polarityEpithelial cellsSpecific membrane proteinsCell dedifferentiationEpithelial cell dedifferentiationE-cadherinRenal epithelial cellsCystic epithelial cellsMembrane proteinsPhenotypic transitionCl(-) cotransporter NKCC1Expression levelsATPaseSecretory isoformEarly cystsTransepithelial secretionImmunocytochemical analysisUltrastructural analysisAutosomal dominant polycystic kidney diseaseDominant polycystic kidney diseasePolycystic kidney disease
1997
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. American Journal Of Physiology 1997, 273: f289-f299. PMID: 9277590, DOI: 10.1152/ajprenal.1997.273.2.f289.Peer-Reviewed Original ResearchConceptsProximal tubulesRat kidneyMonoclonal antibodiesLoop of HenleHigh-resolution immunocytochemical studiesProximal tubule cellsPrevious immunochemical studiesIsoform-specific monoclonal antibodiesNeonatal rat kidneyTubule cellsImmunocytochemical studyThick limbImmunocytochemical techniquesKidneyLAP cellsRenal epitheliumNHE3Immunochemical studiesHigh-resolution immunocytochemical techniqueAdultsLater stagesNephron development
1992
Immunocytochemical characterization of Na(+)-H+ exchanger isoform NHE-1 in rabbit kidney
Biemesderfer D, Reilly RF, Exner M, Igarashi P, Aronson PS. Immunocytochemical characterization of Na(+)-H+ exchanger isoform NHE-1 in rabbit kidney. American Journal Of Physiology 1992, 263: f833-f840. PMID: 1279986, DOI: 10.1152/ajprenal.1992.263.5.f833.Peer-Reviewed Original ResearchConceptsNHE-1 proteinNHE-1Rabbit kidneyDistal convoluted tubuleMultiple nephron segmentsThick ascending limbWestern blot analysisRenal cortexRabbit renal cortexConvoluted tubulesProximal tubulesAscending limbRabbit nephronTubule cellsGuinea pigsLLC-PK1 cellsNephron segmentsIndirect immunofluorescenceKidneyImmunocytochemical characterizationPrincipal cellsExchanger isoformsBlot analysisFusion proteinAntibodiescDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytesAssembly of distinctive coated pit and microvillar microdomains in the renal brush border
Biemesderfer D, Dekan G, Aronson PS, Farquhar MG. Assembly of distinctive coated pit and microvillar microdomains in the renal brush border. American Journal Of Physiology 1992, 262: f55-f67. PMID: 1733297, DOI: 10.1152/ajprenal.1992.262.1.f55.Peer-Reviewed Original Research
1991
Molecular biology of renal Na(+)-H+ exchangers.
Igarashi P, Reilly RF, Hildebrandt F, Biemesderfer D, Rebouças NA, Slayman CW, Aronson PS. Molecular biology of renal Na(+)-H+ exchangers. Kidney International Supplement 1991, 33: s84-9. PMID: 1653876.Commentaries, Editorials and LettersMeSH KeywordsAcidosisAnimalsCarrier ProteinsCell MembraneCloning, MolecularDNAHumansKidneyRabbitsSodium-Hydrogen ExchangersSwineTissue DistributionConceptsKb cDNALLC-PK1 cellsSteady-state transcript levelsPlasma membrane proteinsMembrane-associated domainSequence of cDNACDNA library screeningAmino acid sequenceExchanger gene expressionNorthern blot analysisEvolutionary conservationRenal epithelial cellsCytoplasmic domainMembrane proteinsRegulation of intracellularAcid sequenceTranscript levelsUntranslated regionGene expressionSimilar transcriptsCDNALibrary screeningMolecular biologyPolymerase chain reactionBlot analysis
1985
pH gradient-stimulated sulfate transport by rabbit ileal brush-border membrane vesicles: evidence for SO4-OH exchange
Schron CM, Knickelbein RG, Aronson PS, Della Puca J, Dobbins JW. pH gradient-stimulated sulfate transport by rabbit ileal brush-border membrane vesicles: evidence for SO4-OH exchange. American Journal Of Physiology 1985, 249: g607-g613. PMID: 4061648, DOI: 10.1152/ajpgi.1985.249.5.g607.Peer-Reviewed Original Research