2023
Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion
Chaurasia R, Liang C, How K, Vieira D, Vinetz J. Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion. The Protein Journal 2023, 42: 792-801. PMID: 37653175, DOI: 10.1007/s10930-023-10152-2.Peer-Reviewed Original ResearchMCherry fusion proteinsFusion proteinMCherry tagGene familyMCherry fusionsProtein productionFluorescent fusion proteinsRecombinant protein expressionRecombinant protein productionVM proteinsSuch proteinsFunctional proteinsCell biologyLike domainFast protein liquid chromatographyLeptospiral virulenceSoluble proteinUnique memberPink coloniesProtein scienceStructural predictionsProtein liquid chromatographyRicin BFunctional studiesProtein
2022
Comparison of the PF07598-Encoded Virulence-Modifying Proteins of L. interrogans and L. borgpetersenii
Vieira D, Chaurasia R, Vinetz J. Comparison of the PF07598-Encoded Virulence-Modifying Proteins of L. interrogans and L. borgpetersenii. Tropical Medicine And Infectious Disease 2022, 8: 14. PMID: 36668921, PMCID: PMC9863803, DOI: 10.3390/tropicalmed8010014.Peer-Reviewed Original ResearchVM proteinsEukaryotic cellsGene familyEnvironmental nichesDifferent cladesMammalian hostsSeverity of outbreaksVirulence effectsCellular pathogenesisHuman pathogensMediated TransmissionLeptospiral speciesParalogsPathogenic groupsProteinVaccine-mediated preventionSpeciesL. interrogansL. borgpeterseniiProtein exotoxinsNew strainFatal leptospirosisTarget cellsLeptospirosis pathogenesisInfectious diseasesPathogenic Leptospira Evolved a Unique Gene Family Comprised of Ricin B-Like Lectin Domain-Containing Cytotoxins
Chaurasia R, Marroquin AS, Vinetz JM, Matthias MA. Pathogenic Leptospira Evolved a Unique Gene Family Comprised of Ricin B-Like Lectin Domain-Containing Cytotoxins. Frontiers In Microbiology 2022, 13: 859680. PMID: 35422779, PMCID: PMC9002632, DOI: 10.3389/fmicb.2022.859680.Peer-Reviewed Original ResearchVM proteinsUnique gene familiesStructural homology searchRecombinant ricin B chainΒ-trefoil domainR-type lectinsCaspase-3 activationHeLa cell surfaceBacterial plasmid DNAGene familyImportant virulence determinantN-terminal fragmentHomology searchCell surface bindingRicin B chainHuman cellsNuclear fragmentationB domainHeLa cellsCell surfaceNuclear translocationVirulence determinantsDNase activityDNase functionProtein
2021
Evolutionary Insights into the Microneme-Secreted, Chitinase-Containing High-Molecular-Weight Protein Complexes Involved in Plasmodium Invasion of the Mosquito Midgut
Kaur H, Pacheco MA, Garber L, Escalante AA, Vinetz JM. Evolutionary Insights into the Microneme-Secreted, Chitinase-Containing High-Molecular-Weight Protein Complexes Involved in Plasmodium Invasion of the Mosquito Midgut. Infection And Immunity 2021, 90: e00314-21. PMID: 34606368, PMCID: PMC8788677, DOI: 10.1128/iai.00314-21.Peer-Reviewed Original ResearchConceptsPeritrophic matrixMosquito midgutNew genomic insightsWeight protein complexesHigh-resolution structural modelingDomain-related proteinEvolutionary insightsInvasion machineryGenomic insightsMicronemal proteinsProtein complexesThree-dimensional structureMosquito interactionsGenomic dataPlasmodium invasionProteolytic milieuMidgut epitheliumWeight complexesAdhesive proteinsOokinetesGeneral mechanismProteinChitinasesMidgutChitinaseA Hetero-Multimeric Chitinase-Containing Plasmodium falciparum and Plasmodium gallinaceum Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion
Patra KP, Kaur H, Kolli SK, Wozniak JM, Prieto JH, Yates JR, Gonzalez DJ, Janse CJ, Vinetz JM. A Hetero-Multimeric Chitinase-Containing Plasmodium falciparum and Plasmodium gallinaceum Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion. Frontiers In Cellular And Infection Microbiology 2021, 10: 615343. PMID: 33489941, PMCID: PMC7821095, DOI: 10.3389/fcimb.2020.615343.Peer-Reviewed Original ResearchConceptsChitin-containing peritrophic matrixHMW complexesMosquito midgut invasionDomain-related proteinNext-generation vaccinesMicronemal proteinsVon Willebrand factorProtein complexesMidgut invasionPeritrophic matrixProteolytic milieuMidgut epitheliumPfCHT1Malaria parasitesSurface enolaseOokinetesPlasmodium falciparumWillebrand factorMidgut wallAdhesive proteinsProteinMass spectrometryShort formSize exclusion chromatography dataMosquito vectors
2010
Apical Surface Expression of Aspartic Protease Plasmepsin 4, a Potential Transmission-blocking Target of the Plasmodium Ookinete*
Li F, Patra KP, Yowell CA, Dame JB, Chin K, Vinetz JM. Apical Surface Expression of Aspartic Protease Plasmepsin 4, a Potential Transmission-blocking Target of the Plasmodium Ookinete*. Journal Of Biological Chemistry 2010, 285: 8076-8083. PMID: 20056606, PMCID: PMC2832958, DOI: 10.1074/jbc.m109.063388.Peer-Reviewed Original ResearchConceptsPlasmepsin 4Midgut invasionAspartic proteasesDigestive vacuoleMidgut peritrophic matrixApical surface expressionChitin-binding proteinsMass spectrometry sequencingMalaria parasitesPeritrophic matrixPlasmodium invasionAspartic protease inhibitorsPlasmodium ookinetesParasite infectivityApical surfaceDefinitive hostsMechanistic roleAffinity columnBlood-stage PlasmodiumSurface expressionCalpain inhibitorsMidgut basal laminaProteinVaccine targetsVacuoles
2004
Plasmodium Ookinete-secreted Proteins Secreted through a Common Micronemal Pathway Are Targets of Blocking Malaria Transmission*
Li F, Templeton TJ, Popov V, Comer JE, Tsuboi T, Torii M, Vinetz JM. Plasmodium Ookinete-secreted Proteins Secreted through a Common Micronemal Pathway Are Targets of Blocking Malaria Transmission*. Journal Of Biological Chemistry 2004, 279: 26635-26644. PMID: 15069061, DOI: 10.1074/jbc.m401385200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, WesternCell MembraneChitinasesCulicidaeCytoplasmDNA, ComplementaryElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectImmunohistochemistryLigandsMalariaMicroscopy, ElectronMicroscopy, FluorescenceMicroscopy, ImmunoelectronMolecular Sequence DataPlasmodiumPlasmodium falciparumProtein BindingProtozoan ProteinsReceptors, Cell SurfaceRecombinant ProteinsSequence Homology, Amino AcidSpecies SpecificityConceptsMicroneme secretory organellesDomain-related proteinP. gallinaceumImmunofluorescence localization studiesMicronemal proteinsIndirect immunofluorescence localization studiesSecretory organellesOokinete stagePlasmodium ookinetesLocalization studiesSporogonic stagesParasite infectivityTransmission-blocking vaccine candidateProteinMalaria parasitesPgCHT1Malaria transmission-blocking vaccine candidateApical endA. aegyptiAnopheles mosquitoesAedes aegyptiPlasmodium gallinaceumVon Willebrand factorImmunological targetsVaccine candidates