1999
Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site
Foley J, Wysolmerski J, Missero C, King C, Philbrick W. Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site. Molecular And Cellular Endocrinology 1999, 156: 13-23. PMID: 10612419, DOI: 10.1016/s0303-7207(99)00151-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenovirus E1A ProteinsAnimalsBase SequenceBinding SitesCell Line, TransformedEnhancer Elements, GeneticGene Expression RegulationGenes, rasGenes, ReporterKeratinocytesLuciferasesMiceParathyroid HormoneParathyroid Hormone-Related ProteinPromoter Regions, GeneticProtein IsoformsProteinsProto-Oncogene Protein c-ets-1Proto-Oncogene ProteinsProto-Oncogene Proteins c-etsRNA, MessengerTranscription FactorsTranscription, GeneticConceptsGene expressionBasal promoterReporter constructsEts-1 siteMinimal promoter regionProtein gene expressionTransient transfection assaysReporter gene activityRepressor domainE1A oncoproteinGene activityRas transformationE1A isoformsMurine keratinocyte lineSite upstreamReporter geneTransfection assaysPromoter regionParathyroid hormone-related protein gene expression
1995
Overexpression of parathyroid hormone-related protein or parathyroid hormone in transgenic mice impairs branching morphogenesis during mammary gland development
Wysolmerski J, McCaughern-Carucci J, Daifotis A, Broadus A, Philbrick W. Overexpression of parathyroid hormone-related protein or parathyroid hormone in transgenic mice impairs branching morphogenesis during mammary gland development. Development 1995, 121: 3539-3547. PMID: 8582268, DOI: 10.1242/dev.121.11.3539.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell Culture TechniquesDrug ImplantsEpitheliumFemaleGene ExpressionImmunohistochemistryMammary Glands, AnimalMesodermMiceMice, TransgenicMolecular Sequence DataMorphogenesisParathyroid HormoneParathyroid Hormone-Related ProteinPolymerase Chain ReactionProteinsReceptors, Parathyroid HormoneSignal TransductionConceptsHormone-related proteinBreast developmentTransgenic miceParathyroid hormoneMyoepithelial cellsPTH/PTHrP receptorParathyroid hormone-related proteinMammary glandMammary duct systemOverexpression of PTHrPNormal breast developmentSlow-release pelletsEffects of PTHrPNormal fetal tissuesMammary gland developmentHumoral hypercalcemiaBreast hypoplasiaTumor productsPTHrP receptorNormal miceLobuloalveolar developmentTerminal ductsPTHrPFetal tissuesMice impairs
1993
Identification and characterization of a GC-rich promoter of the human parathyroid hormone-related peptide gene.
Vasavada RC, Wysolmerski JJ, Broadus AE, Philbrick WM. Identification and characterization of a GC-rich promoter of the human parathyroid hormone-related peptide gene. Endocrinology 1993, 7: 273-82. PMID: 8469240, DOI: 10.1210/mend.7.2.8469240.Peer-Reviewed Original ResearchConceptsExon 1cPeptide genesGC-rich promoter elementsCis-regulatory sequencesHuman parathyroid hormone-related peptide geneGC-rich promoterHandful of genesPrimer extension analysisHuman renal carcinoma cellsCanonical TATARenal carcinoma cellsFunctional dissectionCAAT sequencesHuman PTHrP genePutative promoterDNA 5Regulatory sequencesExtension analysisPromoter elementsGene promoterCAT geneGenomic DNAExon 1AAcetyltransferase constructsPrimer extension